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Optimized cycling condition of Cox -1 gene_
| PCR program of Cox -1 | ||||||
|---|---|---|---|---|---|---|
| Hold | 1 Cycles | 35 Cycles | 1 Cycles | Cycles | ||
| Final Extention | Extention | Anneling | Denaturation | Initial Denaturation | STEP | |
| 4 C | 72 C | 72 C | 52 C | 95 C | 95 C | TEMP |
| ∞ | 7 min | 45 sec | 1 min | 30 sec | 3 min | TIME |
List of primers used for PCR amplification of Ascaridia columbae_
| Gene ID | Direction | Sequencing | References |
|---|---|---|---|
| Cox-1 | Cox1-F | 5′-TGGTGGTTTAAGTGTTTGACTG-3′ | Hamzah. et al., 2020 |
| Cox1-R | 5′-CCAACAACAAAGGCAACATT-3′ | ||
| ITS1-5.8s-ITS2 rDNA region | Physa-F | 5′-GCGAAC GGC TCA TTA TAA CA-3′ | Al Quraishy et al., 2020 |
| Physa-R | 5′-AAT TTCACC TCT CAC GCA-3′ |
Optimized cycling condition of ITS1-5_8s-ITS2 rDNA gene_
| PCR program of ITS1-5.8s-ITS2 rDNA | ||||||
|---|---|---|---|---|---|---|
| Hold | 1 Cycles | 35 Cycles | 1 Cycles | Cycles | ||
| Final Extention | Extention | Anneling | Denaturation | Initial Denaturation | STEP | |
| 4 C | 72 C | 72 C | 54 C | 95 C | 95 C | TEMP |
| ∞ | 7 min | 1 min | 1 min | 30 sec | 3 min | TIME |