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Morphological and genetic identification of the gill monogenean parasite (Diclidophora merlangi) that infects Twobar Seabream Fish (Acanthopagrus bifasciatus) in the Arabian Gulf, Saudi Arabia Cover

Morphological and genetic identification of the gill monogenean parasite (Diclidophora merlangi) that infects Twobar Seabream Fish (Acanthopagrus bifasciatus) in the Arabian Gulf, Saudi Arabia

Open Access
|Jul 2024

Figures & Tables

Fig. 1

Photomicrographs of Diclidophora merlangi infecting Acanthopagrus bifasciatus. (A) Whole-mount preparation. (B–K) High magnifications for different body parts, as follows: (B–F) Anterior portion of the prohaptor. (G) Germarium. (H) Testes. (I–K) Clamps in haptor. Note: MO, mouth; BS, buccal sucker; PH, pharynx; GC, gland cells; OE, oesophagus; MCO, male copulatory organ; IC, intestinal crura; V, vitellaria; VR, vitelline reservoir; G, germarium; TE, testes; CL, clamps; HA, haptor; LS, lateral sclerite; MS, median sclerite; ND, nodular openings.
Photomicrographs of Diclidophora merlangi infecting Acanthopagrus bifasciatus. (A) Whole-mount preparation. (B–K) High magnifications for different body parts, as follows: (B–F) Anterior portion of the prohaptor. (G) Germarium. (H) Testes. (I–K) Clamps in haptor. Note: MO, mouth; BS, buccal sucker; PH, pharynx; GC, gland cells; OE, oesophagus; MCO, male copulatory organ; IC, intestinal crura; V, vitellaria; VR, vitelline reservoir; G, germarium; TE, testes; CL, clamps; HA, haptor; LS, lateral sclerite; MS, median sclerite; ND, nodular openings.

Fig. 2.

A consensus phylogenetic tree constructed with maximum likelihood (ML) and Neighbor Joining (NJ) methods from the partial 28S rRNA sequences, showing phylogenetic relationships between Diclidophora merlangi infecting Acanthopagrus bifasciatus (2 sequences (OR889477-OR889478)) and 22 related taxa in NCBI GenBank with Discocotyle sagittata as an outgroup. Numbers indicated at branch nodes are bootstrap values (ML/NJ).
A consensus phylogenetic tree constructed with maximum likelihood (ML) and Neighbor Joining (NJ) methods from the partial 28S rRNA sequences, showing phylogenetic relationships between Diclidophora merlangi infecting Acanthopagrus bifasciatus (2 sequences (OR889477-OR889478)) and 22 related taxa in NCBI GenBank with Discocotyle sagittata as an outgroup. Numbers indicated at branch nodes are bootstrap values (ML/NJ).

Fig. 3.

A consensus phylogenetic tree constructed with maximum likelihood (ML) and Neighbor Joining (NJ) methods from the partial COI sequences, showing phylogenetic relationships between Acanthopagrus bifasciatus (one sequence (OR945709)) and 19 related taxa in NCBI GenBank with Rhabdosargus haffara as an outgroup. Numbers indicated at branch nodes are bootstrap values (ML/NJ).
A consensus phylogenetic tree constructed with maximum likelihood (ML) and Neighbor Joining (NJ) methods from the partial COI sequences, showing phylogenetic relationships between Acanthopagrus bifasciatus (one sequence (OR945709)) and 19 related taxa in NCBI GenBank with Rhabdosargus haffara as an outgroup. Numbers indicated at branch nodes are bootstrap values (ML/NJ).

Comparative metrical data for Diclidophora merlangi and their congeneric species_

Comparable parametersPerdiguero-Alonso et al. 2006Morsy et al. 2018Present study
HostGadus morhuaGadus morhuaMerlangius merlangusSaurida undosquamisAcanthopagrus bifasciatus
LocationNorth SeaCeltic SeaCeltic DeepRed Sea, EgyptArabian Gulf, Saudi Arabia
BodyLength2.243.309.07±1.98 (4.25–13.10)6±2 (4–10)3.67±0.12 (2.97–5.26)
Width1.181.063.69±0.77 (2.12–5.43)1.25±0.23 (0.45–1.39)0.72±0.01 (0.61–0.95)
Buccal sucker diameter---0.12±0.02 (0.08–0.15)0.076±0.004 (0.071–0.089)
Pharynx length0.150.080.27±0.04 (0.15–0.39)0.12±0.006 (0.08–0.15)0.059±0.013 (0.041–0.075)
Muscular bulb diameter0.0770.0690.12±0.02 (0.08–0.15)-0.049±0.008 (0.039–0.055)
Number of copulatory organ hooks171717±2 (13–20)15–1917
Germarium length0.530.781.96±0.43 (1.00–2.83)0.60±0.01 (0.04–0.80)0.232±0.034 (0.191–0.270)
Number of testes223256201±31 (167–290)220±40 (190–250)218±10 (184–267)
Testicular area length0.8260.9080.670±0.031 (0.177–1.413)-0.981±0.023 (0.801–1.26)
Haptor length0.851.152.68±0.63 (1.35–4.10)-0.665±0.128 (0.459–0.752)
First clamp length0.170.220.35±0.05 (0.22–0.43)-0.160±0.008 (0.150–0.166)
First clamp width0.140.170.28±0.40 (0.18–0.36)0.39±0.01 (0.28–0.47)0.142±0.008 (0.133–0.149)
DOI: https://doi.org/10.2478/helm-2024-0012 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
Language: English
Page range: 184 - 193
Submitted on: Mar 4, 2024
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Accepted on: Apr 16, 2024
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Published on: Jul 16, 2024
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2024 G. Alojayri, S. Al-Quraishy, E. Al-Shaebi, O. B. Mohammed, R. Abdel-Gaber, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.