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Relative egg extraction efficiencies of manual and automated fecal egg count methods in equines Cover

Relative egg extraction efficiencies of manual and automated fecal egg count methods in equines

By: L. Britton,  B. Ripley and  P. Slusarewicz  
Open Access
|Apr 2024

Figures & Tables

Fig. 1.

Comparison of Parasight AIO counting algorithm to human analyst enumeration for both strongylid (A) and ascarid (B) ova. Triplicate images for each sample were analyzed both computationally and manually and the averages of each result plotted against each other.
Comparison of Parasight AIO counting algorithm to human analyst enumeration for both strongylid (A) and ascarid (B) ova. Triplicate images for each sample were analyzed both computationally and manually and the averages of each result plotted against each other.

Fig. 2.

Fluorescence imaging of parasite ova. A portion from a representative image taken with the Parasight AIO unit showing both strongylid and ascarid ova. The mesh of the egg chamber is visible in the background. Bar = 200 μm.
Fluorescence imaging of parasite ova. A portion from a representative image taken with the Parasight AIO unit showing both strongylid and ascarid ova. The mesh of the egg chamber is visible in the background. Bar = 200 μm.

Fig. 3.

Comparison of mini-FLOTAC counts against counts of the same samples by Parasight AIO (A), Imagyst (B), McMaster (C) and Wisconsin (D). Each point is the average of three counts of the same sample by each method. Strongyle counts (blue) and ascarid counts (magenta) were plotted separately and fitted by linear regression to provide two lines whose equations and coefficients of determinations are displayed next to them.
Comparison of mini-FLOTAC counts against counts of the same samples by Parasight AIO (A), Imagyst (B), McMaster (C) and Wisconsin (D). Each point is the average of three counts of the same sample by each method. Strongyle counts (blue) and ascarid counts (magenta) were plotted separately and fitted by linear regression to provide two lines whose equations and coefficients of determinations are displayed next to them.

Summary of the average number of eggs counted (EC) across all samples in the dataset for both strongyles and ascarids_ Eggs counted were converted to eggs/gram (EPG) values by multiplication of EC by the appropriate multiplication factor (MF) for the method_ The magnitude of the difference of EC and EPG relative to the mini-FLOTAC (mFT) method are also shown (EC vs_ mFT and EPG vs_ MFT)_

MethodMFStrongyles
Ascarids
ECEPGEC vs mFTEPG vs mFTECEPGEC vs mFTEPG vs mFT
Wisconsin1404.3404.33.590.72282.0282.02.880.58
Parasight1381.2381.23.380.68273.9273.92.800.56
mini-FLOTAC5112.7563.31.001.0097.8489.01.001.00
McMaster2523.9597.80.211.0614.3358.30.150.73
ImagystN/A41.6N/A0.37N/A17.9N/A0.18N/A

Number of ascarid ova observed in each replicate count from the Low group samples for both the McMaster and Imagyst methods_

SampleCount (EPG)McMaster
Imagyst
Rep 1Rep 2Rep 3Rep 1Rep 2Rep 3
A12021000
B10001010
C65702421
D93300327
E107343205
F155161816067
G20310300
H65222551
I98313422
J50111225

Mean numbers of strongylid and ascarid ova counted by each method in each count group and for all groups combined_ n = number of positive samples in the indicated group_

ParasiteGroupnMcMastermini-FLOTACWisconsinImagystParasight
StrongylidsH1048.9220.8822.484.4730.8
M1018.592.2303.431.6339.7
L104.425.187.08.782.5
All3023.9112.7404.341.6381.2

AscaridsH542.1304.4923.751.3941.4
M710.470.7171.016.3134.6
L103.213.538.82.437.6
All2214.397.8282.017.9273.9
DOI: https://doi.org/10.2478/helm-2024-0007 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
Language: English
Page range: 20 - 29
Submitted on: Dec 12, 2023
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Accepted on: Feb 18, 2024
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Published on: Apr 23, 2024
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open

© 2024 L. Britton, B. Ripley, P. Slusarewicz, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.