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Morphological and molecular characterizations of Pratylenchus coffeae infecting Ming aralia and coffee in Vietnam Cover

Morphological and molecular characterizations of Pratylenchus coffeae infecting Ming aralia and coffee in Vietnam

Open Access
|Apr 2024

Figures & Tables

Fig. 1.

Light microscopy pictures of Pratylenchus coffeae from this study. A-D, F-H: Female. A: Entire body, B: Anterior part of the body showing lip and pharynx region; C: Anterior end region; D: Tail region; F: Lateral field at mid-body; G: Vulva region showing spermatheca; H: vulva and post-uterine sac. E: Tail region of the male. (Scale bar: A: 50 μm; B-H: 10 μm).
Light microscopy pictures of Pratylenchus coffeae from this study. A-D, F-H: Female. A: Entire body, B: Anterior part of the body showing lip and pharynx region; C: Anterior end region; D: Tail region; F: Lateral field at mid-body; G: Vulva region showing spermatheca; H: vulva and post-uterine sac. E: Tail region of the male. (Scale bar: A: 50 μm; B-H: 10 μm).

Fig. 2.

Cluster and MDS analyses of Pratylenchus coffeae from 17 populations in the world.
Cluster and MDS analyses of Pratylenchus coffeae from 17 populations in the world.

Fig. 3.

Bayesian phylogenetic tree of Pratylenchus species generated from D2D3 of 28S rRNA genes using the HKY+G model. Posterior Probability support was given next to each node. The sequences from this study were marked by red and bold.
Bayesian phylogenetic tree of Pratylenchus species generated from D2D3 of 28S rRNA genes using the HKY+G model. Posterior Probability support was given next to each node. The sequences from this study were marked by red and bold.

Fig. 4.

Bayesian phylogenetic tree of Pratylenchus species generated from COI mtDNA genes using the HKY+G model. Posterior Probability support was given next to each node. The sequences from this study were marked by red and bold.
Bayesian phylogenetic tree of Pratylenchus species generated from COI mtDNA genes using the HKY+G model. Posterior Probability support was given next to each node. The sequences from this study were marked by red and bold.

Measurements of Pratylenchus coffeae from Ming aralia and coffee in Vietnam_

CharactersPratylenchus coffeae (Zimmermann, 1898) Filipjev & Schuurmans Stekhoven, 1941

Host plantMing araliaCoffee

SexFemaleMaleFemaleMale
n10101010
a23 ± 2.4 (22 – 28)28.6 ± 1.2 (27.6 – 30.7)27 ± 1.4 (25.5 – 29.1)31 ± 2.9 (25.9 – 36.4)
b6.0 ± 0.3 (5.7 – 6.5)5.9 ± 0.2 (5.7 – 6.2)6 ± 0.3 (5.7 – 6.5)6 ± 0.3 (5.7 – 6.4)
b′4.2 ± 0.2 (4.1 – 4.5)4.2 ± 0.2 (4 – 4.6)4 ± 0.2 (3.8 – 4.4)4.2 ± 0.2 (3.8 – 4.5)
c18.8 ± 0.5 (18.3 – 19.6)19.5 ± 1.4 (18.3 – 21)19.2 ± 1.4 (17.4 – 22)20 ± 1.5 (18.5 – 23)
c′1. 9 ± 0.1 (1.7 – 2.1)2.3 ± 0.2 (2 – 2.7)2.2 ± 0.2 (1.9 – 2.5)2.1 ± 0.2 (1.9 – 2.4)
Body length (L)519 ± 25 (485 – 550)497 ± 24 (476 – 542)473 ± 28 (440 – 531)494 ± 26.7 (454 – 533)
V79 ± 3 (74 – 82)80 ± 2 (78 – 84)
Stylet length15.9 ± 0.2 (15.7 – 16.1)14.5 ± 0.3 (13.9 – 14.9)15.9 ± 0.4 (15.3 – 16.6)14.7 ± 0.8 (13.8 – 16)
Dorsal gland opening from stylet base (DGO)3.2 ± 0.7 (2.2 – 3.9)3.3 ± 0.3 (2.9 – 3.6)2.9 ± 0.4 (2.3 – 3.3)3.1 ± 0.4 (2.5 – 3.7)
Anterior end to valve of median bulb56 ± 3 (52 – 58)53 ± 1.4 (52 – 55)50 ± 3.4 (46 – 58)52 ± 1.8 (49 – 55)
Anterior end to nerve ring70 ± 3.3 (64 – 72)65 ± 3 (63 – 69)61 ± 4 (57 – 70)64 ± 2 (61 – 68)
Anterior end to secretory-excretory pore80 ± 2 (78 – 82)76 ± 3 (72 – 79)75 ± 5 (69 – 81)76 ± 2 (72 – 79)
Anterior end to pharyngo-intestinal junction87 ± 5 (79 – 91)84 ± 2 (81 – 87)79 ± 5 (74 – 89)82 ± 4 (74 – 86)
Anterior end to end of pharyngeal gland123 ± 8 (109 – 129)118 ± 6 (108 – 124)117 ± 5 (109 – 123)119 ± 7 (109 – 127)
Pharyngeal gland overlap36 ± 4 (30 – 39)34 ± 5 (27 – 42)38 ± 4 (31 – 44)37 ± 4 (31 – 43)
Max body diam.22 ± 1.5 (20 – 24)17.4 ± 0.7 (16.3 – 18.5)17.5 ± 1 (16.2 – 19.3)16.1 ± 2.1 (13.6 – 19.6)
Vulval body diam.19.9 ± 1 (19 – 22)16.3 ± 1.4 (14.4 – 19.4)
Post-uterine sac (PUS)21 ± 4 (16.4 – 26)27 ± 2 (24 – 30)
Anal body diam.14.7 ± 0.3 (14.3 – 15)11 ± 0.4 (10.3 – 11.6)11.3 ± 0.4 (10.7 – 12)11.4 ± 1 (10.1 – 12.9)
Tail length28 ± 2 (25 – 30)26 ± 2 (23 – 30)25 ± 2 (21 – 27)24 ± 2 (21 – 28)
Spicule length18.4 ± 1 (17.2 – 19.9)17.8 ± 1 (16.1 – 19.3)
Gubernaculum length4.5 ± 0.3 (3.9 – 4.8)4.3 ± 0.6 (3.5 – 5.5)
DOI: https://doi.org/10.2478/helm-2024-0004 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
Language: English
Page range: 76 - 84
Submitted on: Jul 1, 2023
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Accepted on: Jan 19, 2023
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Published on: Apr 23, 2024
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open

© 2024 H. D. Nguyen, H. T. Nguyen, T. M. L. Le, D. D. Hoang, T. D. Nguyen, Q. P. Trinh, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.