Morphological and molecular characterization of Haemonchus contortus isolated from the small ruminants of south Gujarat, India Cover

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Morphological and molecular characterization of Haemonchus contortus isolated from the small ruminants of south Gujarat, India

Volume 60 (2023): Issue 2 (June 2023)

By: B. Das, N. Kumar, J. B. Solanki, M. M. Jadav and I. H. Kalyani

Open Access

|Sep 2023

Figures & Tables

Bursa of male H. contortus showing Y shaped dorsal ray and each spicule with a single barb at its distal tip.

Intertwining of red intestine and white ovary of H. contortus female

a: Linguiform type B vulvar flap of H. contortus female; b: Linguiform type I vulvar flap of H. contortus female

Knobbed/button type vulvar flap of H. contortus female.

Smooth type vulvar flap of H. contortus female

PCR amplification of ITS-1 of 198 bp (lane 1 and 3) and ITS-2 of 350 bp (lane 2 and 4) genotype sequence of Navsari isolates of H. contortus. Lane M: 100 bp DNA ladder.

Phylogenetic relationship of Haemonchus along with some other important nematodes based on ITS-1 sequences.

Phylogenetic relationship of Haemonchus along with some other important nematodes based on ITS-2 plus sequences.

Multiple alignment percent identity matrix of ITS-1 of H_ contortus_

	1	2	3	4	5	6	7	8	9
2	100.0
3	97.0	97.0
4	95.8	95.8	98.8
5	96.3	96.3	98.8	98.8
6	96.3	96.3	98.8	98.8	100.0
7	96.3	96.3	98.8	98.8	100.0	100.0
8	96.4	96.4	99.4	99.4	99.4	99.4	99.4
9	95.7	95.7	98.8	98.8	98.1	98.1	98.1	99.4
10	95.2	95.2	98.2	99.4	98.1	98.1	98.1	98.8	98.2

Multiple alignment percent identity matrix of ITS-2 plus of H_ contortus_

	1	2	3	4	5	6	7	8	9	10	11	12
2	100.0
3	100.0	100.0
4	99.1	99.1	99.0
5	98.4	98.4	98.4	98.4
6	99.5	99.5	99.5	98.4	97.8
7	99.5	99.5	99.5	99.5	97.8	98.9
8	98.0	98.0	98.0	99.0	97.8	97.3	98.5
9	100.0	100.0	100.0	99.0	98.4	99.5	99.5	98.0
10	99.6	99.6	99.5	98.7	97.8	98.9	99.0	97.5	99.5
11	99.5	99.5	99.5	99.5	97.8	98.9	100.0	98.5	99.5	99.0
12	96.5	96.5	96.5	95.5	94.6	95.6	96.0	94.5	96.5	96.0	96.0
13	99.0	99.0	99.0	100.0	98.4	98.4	99.5	99.0	99.0	98.5	99.5	95.5

Distribution pattern of vulvar morphotypes of H_ contortus in the small ruminants_

Host	Female worms (No.)		Vulvar morphotypes						p value

	Recovered	Examined	Linguiform		Knobbed		Smooth
	Recovered	Examined	No.	%	No.	%	No.	%
Goat	2408	2061	342	16.6	1602	77.7	117	5.7	0.045
Sheep	730	581	125	21.5	423	72.8	33	5.7	0.05
Total	3138	2642	467	17.7	2025	76.6	150	5.7	0.05

Primers used for the amplification of the DNA of the nematode_

Name of gene	Name of primers	Primers sequence	Product length	References
ITS1	Forward	5′-TATGACATGAGCCGTTCGAG-3′	198 bp	Bandyopadhyay et al., 2011
ITS1	Reverse	5′-TGATCATTAAGGTTCCCCGA-3	198 bp	Bandyopadhyay et al., 2011
ITS2 plus	Forward	5′-ACGTCTGGTTCAGGGTTGTT-3′	350 bp	Stevenson et al., 1995
ITS2 plus	Reverse	5′-TTAGTTTCTTTTCCTCCGCT-3′	350 bp	Stevenson et al., 1995

DOI: https://doi.org/10.2478/helm-2023-0020 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605

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Language: English

Page range: 175 - 188

Submitted on: Nov 29, 2022

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Accepted on: May 30, 2023

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Published on: Sep 22, 2023

Published by: Slovak Academy of Sciences, Institute of Parasitology

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

Small ruminants,

Vulvar morphology,

Related subjects:

Life sciences, other,

Clinical medicine,

Microbiology, virology and infection epidemiology

© 2023 B. Das, N. Kumar, J. B. Solanki, M. M. Jadav, I. H. Kalyani, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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