Fig. 1.
Cysts identified from oryx antelopes (EC = E_ canadensis G6/7, EO = E_ ortleppi, na = not amplifiable)_
| n cysts examined | Echinococcus species | |
|---|---|---|
| Oryx A | 11 (lungs, fertile) | EC (n=10), EO (n=1) |
| 1 (liver, sterile) | na (n=1) | |
| Oryx B | 4 (liver, sterile) | EO (n=2), na (n=2) |
Primer pairs used for PCRs (Hüttner et al_, 2008)_
| Target | Primers for first PCR (5′-3′) | Primers for nested PCR (5′-3′) |
|---|---|---|
| Complete nad1 gene | F: TGGAACTCAGTTTGAGCTTTACTA | F: TATTAAAAATATTGAGTTTGCGTC |
| R: ATATCAAAGTAACCTGCTATGCAG | R: TCTTGAAGTTAACAGCATCACGAT | |
| ~190 bp fragment of nad1 | F: TGTTTTTGAGATCAGTTCGGTGTG | F: CAGTTCGGTGTGCTTTTGGGTCTG |
| R: CATAATCAAACGGAGTACGATTAG | R: GAGTACGATTAGTCTCACACAGCA |
Number of eggs and Echinococcus species identified from faecal samples_
| Host species | Number of isolated taeniid eggs | Number of successful amplified and sequenced eggs | species identified (number of eggs) |
|---|---|---|---|
| Dog A | 2 | - | - |
| Dog B | 10 | 4 | E. canadensis G6/7 (4) |
| Dog C | 7 | - | - |
| Dog D | 10 | - | - |
| Dog E | 2 | - | - |
| Dog F | 3 | - | - |
| Dog G | 2 | - | - |
| Dog H | 7 | - | - |
| Dog I | 2 | - | - |
| Jackal A | - | - | - |
| Jackal B | 30 | 6 | E. canadensis G6/7 (6) |
| Jackal C | - | - | - |
| Jackal D | 19 | 2 | E. canadensis G6/7 (2) |
| Jackal E | 10 | - | - |