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Diagnostic accuracy of swine echinococcosis cytopathological tests and challenges for a differential diagnosis: slaughterhouse data Cover

Diagnostic accuracy of swine echinococcosis cytopathological tests and challenges for a differential diagnosis: slaughterhouse data

Open Access
|Jun 2023

Figures & Tables

Fig. 1.

Flow chart of the diagnostic accuracy study.
Flow chart of the diagnostic accuracy study.

Fig. 2.

Kidney echinococcosis caused by Echinococcus granulosusA: Hydatid cyst in kidney, swine. B: Broad capsule of hydatid cyst with typical striated and trilaminated layer and that do not contain calcification. Note a protoscolisce (arrow). HES stain. C: Protoscolice (arrow), with inside little calcareous corpuscles (arrow head). Protoscolisce is surrounded by almost no inflammatory reaction composed of few lymphocytes and macrophages (stars). There are no neutrophils.
Kidney echinococcosis caused by Echinococcus granulosusA: Hydatid cyst in kidney, swine. B: Broad capsule of hydatid cyst with typical striated and trilaminated layer and that do not contain calcification. Note a protoscolisce (arrow). HES stain. C: Protoscolice (arrow), with inside little calcareous corpuscles (arrow head). Protoscolisce is surrounded by almost no inflammatory reaction composed of few lymphocytes and macrophages (stars). There are no neutrophils.

Fig. 3.

Diagnostic accuracy for detection of swine echinococcosis between histopathological test and cytopathological test 1 (Ziehl stain with epifluorescence microscope).
Diagnostic accuracy for detection of swine echinococcosis between histopathological test and cytopathological test 1 (Ziehl stain with epifluorescence microscope).

Fig. 4.

Diagnostic accuracy for detection of swine echinococcosis between histopathological test and cytopathological test 2 (Ziehl Neelsen stain with transmitted light microscope).
Diagnostic accuracy for detection of swine echinococcosis between histopathological test and cytopathological test 2 (Ziehl Neelsen stain with transmitted light microscope).

Fig. 5.

Cryptococcus neoformans hepatitis (5.A: HES Stain; 5.B: PAS stain: There are many budding yeasts (arrow); 5.C: Grocott Methenamine Silver stain).
Cryptococcus neoformans hepatitis (5.A: HES Stain; 5.B: PAS stain: There are many budding yeasts (arrow); 5.C: Grocott Methenamine Silver stain).

Diagnostic accuracy of cytopathological test 1 (Ziehl staining with the epifluorescence microscope) and cytopathological test 2 (Ziehl Neelsen staining with the transmitted light microscope) in the detection of clinical swine echinococcosis_

Cythopath 1% [95% CI]Cythopath 2% [95% CI]Test ratio[95% CI]p value
Specificity100 [100 – 100]100 [100 – 100]1 [1 – 1]
Sensitivity79.66[69.39 – 89.93]66.10 [54.02 – 78.18]1.2 [0.88 – 1.66]0.059
PPV100 [100 – 100]100 [100 – 100]1 [1 – 1]
NPV40 [18.53 – 61.47]28.57 [11.84 – 45.3]1.4 [0.41 – 5.2]0.06

Nature of infected organs in swine echinococcosis diagnosed in Côte_

ParasiteInfected organs

Liver (%)Lung (%)Kidney (%)
Echinococcus granulosus1753
Echinococcus spp.002
Total1 (1.6)7 (11.1)55 (87.3)

Occurrence of diseases that could be misdiagnosed as swine echinococcosis in Côte d’Ivoire

DiseasesInvolved organs

LiverLungKidney
Ascaris suum infection or migration42
Cysticercus tenuicollis hepatitis1
Fasciolosis1
Cryptococcus neoformans infection1
Cirrhosis3
Neoplasms1(Hepatocellular carcinoma)01(Small clear cell carcinoma)
Hydronephrosis 4
Actinobacillus spp or Mycoplasma spp. Pneumonia or Pleuropneumonia 11
Inflammatory conditions of unknown aetiology 2
Abscess 3

Total11167
DOI: https://doi.org/10.2478/helm-2023-0004 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
Language: English
Page range: 52 - 62
Submitted on: Apr 27, 2022
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Accepted on: Dec 12, 2022
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Published on: Jun 4, 2023
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open

© 2023 A. Toure, L. Toure, G. L. Acapovi-Yao, C. B. V. Senin, N. Kone, M. Kachani, E. Couacy-Hymann, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.