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Inducing systemic acquired resistance (SAR) against root-knot nematode Meloidogyne javanica and evaluation of biochemical changes in cucumber root Cover

Inducing systemic acquired resistance (SAR) against root-knot nematode Meloidogyne javanica and evaluation of biochemical changes in cucumber root

Helminthologia
Volume 59 (2022): Issue 4 (December 2022)
By: I. E. Taher and  S. N. Ami  
Open Access
|Dec 2022

Figures & Tables

Fig. 1

Effect of salicylic acid, ascorbic acid, and Silicon application on total phenolic substances (mg/0.5g root) in Cucumber root infected by Meloidogyne javanica. * Co1 = healthy plant, Co2 = control, (R) = root application, (R+F) = root and foliar application, AA = ascorbic acid. Si = silicon and SA = salicylic acid. *Each number is means of three replications. * Means followed by different letter(s) differ significantly according Duncan’s multiple range test, P ≤ 0.05.
Effect of salicylic acid, ascorbic acid, and Silicon application on total phenolic substances (mg/0.5g root) in Cucumber root infected by Meloidogyne javanica. * Co1 = healthy plant, Co2 = control, (R) = root application, (R+F) = root and foliar application, AA = ascorbic acid. Si = silicon and SA = salicylic acid. *Each number is means of three replications. * Means followed by different letter(s) differ significantly according Duncan’s multiple range test, P ≤ 0.05.

Fig. 2

Effect of salicylic acid, ascorbic acid, and Silicon application on peroxidase activity (Unit. Min-1.gm-1 f w.) in Cucumber root infected by Meloidogyne javanica. * Co1 = healthy plant, Co2 = control, (R) = root application, (R+F) = root and foliar application, AA = ascorbic acid. Si = silicon and SA = salicylic acid. *Each number is means of three replications. * Means followed by different letter(s) differ significantly according Duncan’s multiple range test, P ≤ 0.05
Effect of salicylic acid, ascorbic acid, and Silicon application on peroxidase activity (Unit. Min-1.gm-1 f w.) in Cucumber root infected by Meloidogyne javanica. * Co1 = healthy plant, Co2 = control, (R) = root application, (R+F) = root and foliar application, AA = ascorbic acid. Si = silicon and SA = salicylic acid. *Each number is means of three replications. * Means followed by different letter(s) differ significantly according Duncan’s multiple range test, P ≤ 0.05

Fig. 3

Effect of salicylic acid, ascorbic acid, and Silicon application on polyphenol oxidase activity (Unit. Min-1.gm-1 f w.) in Cucumber root infected by Meloidogyne javanica. * Co1 = healthy plant, Co2 = control, (R) = root application, (R+F) = root and foliar application, AA = ascorbic acid. Si = silicon and SA = salicylic acid. *Each number is means of three replications. * Means followed by different letter(s) differ significantly according Duncan’s multiple range tests, P ≤ 0.05.
Effect of salicylic acid, ascorbic acid, and Silicon application on polyphenol oxidase activity (Unit. Min-1.gm-1 f w.) in Cucumber root infected by Meloidogyne javanica. * Co1 = healthy plant, Co2 = control, (R) = root application, (R+F) = root and foliar application, AA = ascorbic acid. Si = silicon and SA = salicylic acid. *Each number is means of three replications. * Means followed by different letter(s) differ significantly according Duncan’s multiple range tests, P ≤ 0.05.

Effect of salicylic acid, ascorbic acid and (Silicon + Calcium) application on growth criteria of Cucumber infected by Meloidogyne javanica_

Growth Criteria
TreatmentsChlorophyll (Spad)Shoots fresh weight (g)Root fresh weight (g)Shoots Dry weight (g)Root Dry weight (g)Shoots length (cm)Root length (cm)
Healthy plant17.17 a61.13 a17.17 a10.787 a1.089 a71.3 a32.37 a
salicylic acid (R+F)14.97 b53.7 b14.97 b9.373 b0.9507 b63.37 b23.9 b
salicylic acid (R)14.03 c51.8 bc14.03 c9.03 bc0.8627 c61.3 b21.47 c
salicylic acid (F)13.6 c50.97 bcd13.6 c8.857 bcd0.8313 cd57.57 c21.03 c
Silicon (R+F)12.9 d48.3 cde12.9 d8.333 cde0.81 de56.2 cd19.9 d
Silicon (R)12.2 e47.67 cde12.2 e8.153 de0.7663 ef55.57 cd19.63 d
Ascorbic acid (R+F)12.17 e46.7 de12.17 e8.03 e0.759 f53.2 de19.63 d
Ascorbic acid (R)12.13 e46.5 de12.13 e8.007 e0.7413 f53.17 de19.23 d
Silicon (F)11.53 ef46.37 e11.53 ef7.99 e0.7157 fg52.9 de19 de
Ascorbic acid (F)11.1 f45.83 e11.1 f7.983 e0.6857 g50.07 e18.87 de
Control (Infested, non-treated )9.3 g41.03 f9.3 g7.017 f0.5837 h44.9 f18.07 e

Influence of salicylic acid, ascorbic acid, and Silicon application on infection criteria of Cucumber infected by Meloidogyne javanica_

Infection Criteria
TreatmentsNo. of root knot (galls)Root gall indexNo. of egg mass/root systemNo. of nematodes/root systemNo. of eggs/root systemNo. of nematodes / pots soilFinal population density of nematodesRate of reproduction
Healthy plant0.00 a0.000 a0.00 a0.0 a0 a0.00 a0.000 a0.000 a
salicylic acid (R+F)23.00 b3.000 b10.33 b395.7 b2196b477.0 b3068 b3.068 b
Silicon (R+F)25.67 bc3.000 bc13.00 bc404.3 bc2765bc584.3 c3230 bc3.230 bc
salicylic acid (R)26.00 bc3.000 bc15.33 cd415.0 bc3262cd711.7 d3754 bcd3.754 bcd
salicylic acid (F)28.33 cd3.000 bc16.33 cde418.3 bc3521cde721.3 d4418 bcde4.418 bcde
Ascorbic acid (R)29.00 cd3.000 bc17.33 de418.7 bc3651de772.7 e4839 de4.839 de
Ascorbic acid (R+F)30.67 cd3.000 bc17.33 de419.7 bc3693de789.3 e4842 cde4.842 cde
Silicon (R)30.67 cd3.333 b17.67 de443.7 cd3702de841.0 f4953 de4.953 de
Ascorbic acid (F)33.33 de4.000 d18.33 de461.7 de3935de848.7 f5252 de5.252 de
Silicon (F)33.33 de4.000 d20.00 e468.0 de4258e859.7 f5573 e5.573 e
Control (Infested, non-treated)37.33 e4.000 d26.00 f499.3 e5984f874.7 f7492 f7.492 f
DOI: https://doi.org/10.2478/helm-2022-0042 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
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Language: English
Page range: 404 - 413
Submitted on: Apr 25, 2022
Accepted on: Nov 30, 2022
Published on: Dec 30, 2022
Published by: Slovak Academy of Sciences, Institute of Parasitology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
SAR,
Salicylic acid,
ascorbic acid,
Si,
polyphenol oxidase,
peroxidase,
total phenol
Related subjects:
Life sciences,
Zoology,
Ecology,
Life sciences, other,
Medicine,
Clinical medicine,
Microbiology, virology and infection epidemiology

© 2022 I. E. Taher, S. N. Ami, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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