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Occurrence and phylogenetic description of cystic echinococcosis isolate from Egyptian camel (Camelus dromedarius) Cover

Occurrence and phylogenetic description of cystic echinococcosis isolate from Egyptian camel (Camelus dromedarius)

Helminthologia
Volume 59 (2022): Issue 3 (September 2022)
By: I. S. Elshahawy,  M. A. El-Seify,  Z. K. Ahamed and  M. M. Fawaz  
Open Access
|Dec 2022

Figures & Tables

Fig. 1

a, b) Calcified cyst; c) Protoscoleces; d) Multiple fertile cysts in the lung (yellow arrow head).
a, b) Calcified cyst; c) Protoscoleces; d) Multiple fertile cysts in the lung (yellow arrow head).

Fig. 2

Agarose (1.5%) gel electrophoresis of PCR-products of cox1 gene (450bp) of E. granulosus isolates in camel samples. L: ladder, Pos.: positive control, Neg.: negative control, 1, 2, 3, 4and 5 samples (all +ve).
Agarose (1.5%) gel electrophoresis of PCR-products of cox1 gene (450bp) of E. granulosus isolates in camel samples. L: ladder, Pos.: positive control, Neg.: negative control, 1, 2, 3, 4and 5 samples (all +ve).

Fig. 3

Agarose (1.5%) gel electrophoresis of PCR-products of Nad1 gene (500bp) of E. granulosus isolates in camel samples. L: ladder, Pos.: positive control, Neg.: negative control, 1, 2, 3,4and 5 samples (all +ve).
Agarose (1.5%) gel electrophoresis of PCR-products of Nad1 gene (500bp) of E. granulosus isolates in camel samples. L: ladder, Pos.: positive control, Neg.: negative control, 1, 2, 3,4and 5 samples (all +ve).

Fig. 4

Genetic relationships of the obtained genotypes from camel in the present study and reference sequences related genotypes of E. granulosus as well as Taenia saginata as the outgroup. The relationships were inferred based on phylogenetic tree (cox1 gene). The phylogenetic tree was constructed using Maximum Likelihood Tree implemented in MEGA software version 6.
Genetic relationships of the obtained genotypes from camel in the present study and reference sequences related genotypes of E. granulosus as well as Taenia saginata as the outgroup. The relationships were inferred based on phylogenetic tree (cox1 gene). The phylogenetic tree was constructed using Maximum Likelihood Tree implemented in MEGA software version 6.

Fig. 5

Genetic relationships of the obtained genotypes from camel in the present study and reference sequences related genotypes of E. granulosus as well as Taenia saginata as the out-group. The relationships were inferred based on phylogenetic tree (nad1 gene). The phylogenetic tree was constructed using Maximum Likelihood Tree implemented in MEGA software version 6.
Genetic relationships of the obtained genotypes from camel in the present study and reference sequences related genotypes of E. granulosus as well as Taenia saginata as the out-group. The relationships were inferred based on phylogenetic tree (nad1 gene). The phylogenetic tree was constructed using Maximum Likelihood Tree implemented in MEGA software version 6.

Difference in bases at the nad1 locus of E_ granulosus s_l_

Accession No.GenotypeNucleotide Substitution No.Substitution/PositionSubstitution/PositionSubstitution/Position
(MH301013)G7 (Pig, France)ThreeG to A 180/381/387--
(KX010880)G6/7 (Cattle, Ethiobia)FourG to A 180 / 381C to T 336A to G 432
(MT166290)G6 (Camel, Nigeria)FiveG to A 180 /381/387C to T 336A to G 432

Comparative biochemical analysis of hydatid fluids collected from dromedary camels and cattle infected with cystic echinococcosis (mean ± SE, n = 5)_

Biochemical profilesHost
CamelCattle
Total protein (g/l)3.76 ± 0.732.42 ± 0.01
Glucose (mmol/L)3.36 ± 0.162.58 ± 0.47
Urea (mmol/L)5.21 ± 0.174.36 ± 0.5
Cholesterol (mmol/L)2.94 ± 0.612.19 ± 0.31
Creatinine (μmol/L) Calcium (mmol/L)101.22 ± 3.09 2.937 ± 0.0380.67 ± 0.66 3.224 ± 0.02
Triglycerides (mmol/L)0.039 ± 0.010.186 ± 0.003
Sodium (mmol/L)111.35 ± 5.45118.09 ± 0.54
Potassium (mmol/L)13.92 ± 7.4110.05 ± 0.05
Magnesium (mmol/L)1.113 ± 0.331.019 ± 0.02
Copper (ppm)0.42 ± 0.110.338 ± 0.016

GenBank accession numbers of cox1 and nad1 of Echinococcus genotypes used in phylogenetic analysis in the current study

Accession No. for NAD1LocalityHost originGenotypeAccession No. for Cox1LocalityHost originGenotype
MW183240EgyptCamelG7*MW173485EgyptCamelG7*
MW183239EgyptCamelG7*MW173484EgyptCamelG7*
MH301013FrancePigG7MH301013FrancePigG7
KX010897HungaryPigG6/7MH301019ItalyPigG7
KX010890FrancePigG6/7MH301010FrancePigG7
KX010889NamibiaOryxG6/7MH301009FrancePigG7
KX010886SudanCamelG6/7MH301008FrancePigG7
MH301016FrancePigG7MH300939SudanCamelG6
KX510135SerbiaPigG6/7KX010833KenyaCamelG6/7
KX231668ArmeniaPigG6/7MT166286NigeriaCamelG6
KX010880EthiobiaCattleG6/7MT166284NigeriaCattleG6
MT525964KenyaCattleG6/7MH301021UkrainePigG6
MT166290NigeriaCamelG6MH301006PolandPigG7
MT166289NigeriaCattleG6MH301005PolandHomosapiensG7
JN191326EgyptDonkeyG4JN191319EgyptDonkeyG4
MK644934South KoreaHumanTaenia saginataMK644934South KoreaHumanTaenia saginata
DOI: https://doi.org/10.2478/helm-2022-0026 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
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Language: English
Page range: 253 - 264
Submitted on: Feb 3, 2022
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Accepted on: Aug 17, 2022
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Published on: Dec 17, 2022
Published by: Slovak Academy of Sciences, Institute of Parasitology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Cystic echinococcosis,
Camelus dromedarius,
Egypt,
Epidemiological status,
Molecular characterization
Related subjects:
Life sciences,
Zoology,
Ecology,
Life sciences, other,
Medicine,
Clinical medicine,
Microbiology, virology and infection epidemiology

© 2022 I. S. Elshahawy, M. A. El-Seify, Z. K. Ahamed, M. M. Fawaz, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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