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Does in vitro and in vivo exposure to medicinal herbs cause structural cuticular changes in Haemonchus contortus? Cover

Does in vitro and in vivo exposure to medicinal herbs cause structural cuticular changes in Haemonchus contortus?

Helminthologia
Volume 59 (2022): Issue 3 (September 2022)
By: M. Komáromyová,  D. Barčák,  A. Königová,  M. Urda Dolinská and  M. Várady  
Open Access
|Dec 2022

Figures & Tables

Fig. 1

Scanning electron photomicrographs of Haemonchus contortus from the in vivo experimental groups. H1–H4 depict specimens collected from lambs fed a diet enriched with a Herbmix extract, and C1–C4 depict specimens from the control animals. H1 and C1, cephalic end of the body, both females; H2 and C2, region near the cervical papillae, both females; H3 and C3, vulvar region of the body, both females; H4 and C4, posterior end with the copulatory bursa, both males. Note the cephalic end of the female body (C1) and the posterior part of the male body (H4), with several artificial folds (asterisk), small protuberances (arrowhead) in the region anterior to the base of a cervical papilla (H2) and weak ridges (arrow) on the cuticle in the vulvar region (H3).
Scanning electron photomicrographs of Haemonchus contortus from the in vivo experimental groups. H1–H4 depict specimens collected from lambs fed a diet enriched with a Herbmix extract, and C1–C4 depict specimens from the control animals. H1 and C1, cephalic end of the body, both females; H2 and C2, region near the cervical papillae, both females; H3 and C3, vulvar region of the body, both females; H4 and C4, posterior end with the copulatory bursa, both males. Note the cephalic end of the female body (C1) and the posterior part of the male body (H4), with several artificial folds (asterisk), small protuberances (arrowhead) in the region anterior to the base of a cervical papilla (H2) and weak ridges (arrow) on the cuticle in the vulvar region (H3).

Fig. 2

Dose-response relationship of the Herbmix aqueous extract against Haemonchus contortus in the EHT.
Dose-response relationship of the Herbmix aqueous extract against Haemonchus contortus in the EHT.

Fig. 3

Scanning electron photomicrographs of Haemonchus contortus from the in vitro experimental groups. H1–H3 depict specimens treated with the Herbmix extract diluted in PBS, and C1–C3 depict specimens from the control group kept in PBS. H1 and C1, cephalic end of the body; H2 and C2, region around a cervical papilla; H3 and C3, vulvar region with a lateral button. Note the slightly sinuous physiological longitudinal ridges (arrows), weak transverse wrinkles (arrowheads) and artifacts of sample preparation (asterisks).
Scanning electron photomicrographs of Haemonchus contortus from the in vitro experimental groups. H1–H3 depict specimens treated with the Herbmix extract diluted in PBS, and C1–C3 depict specimens from the control group kept in PBS. H1 and C1, cephalic end of the body; H2 and C2, region around a cervical papilla; H3 and C3, vulvar region with a lateral button. Note the slightly sinuous physiological longitudinal ridges (arrows), weak transverse wrinkles (arrowheads) and artifacts of sample preparation (asterisks).

Hatching and ovicidal activity at the concentrations of the Herbmix aqueous extract tested in the EHT_

Herbmix concentration (mg/mL)Ovicidal effect (%)Hatching (%)p value
258317< 0.001
12.57129< 0.001
6.254554< 0.001
3.1253466< 0.001
1.5631585< 0.001
Control199-
DOI: https://doi.org/10.2478/helm-2022-0023 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
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Language: English
Page range: 265 - 274
Submitted on: May 16, 2022
|
Accepted on: Aug 30, 2022
|
Published on: Dec 17, 2022
Published by: Slovak Academy of Sciences, Institute of Parasitology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
medicinal plants,
herbal mixtures,
antiparasitic effect,
scanning electron microscopy
Related subjects:
Life sciences,
Zoology,
Ecology,
Life sciences, other,
Medicine,
Clinical medicine,
Microbiology, virology and infection epidemiology

© 2022 M. Komáromyová, D. Barčák, A. Königová, M. Urda Dolinská, M. Várady, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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