Have a personal or library account? Click to login
Molecular and morphological characterization of the root -lesion nematode, Pratylenchus neglectus, on corn from Henan Province of China Cover

Molecular and morphological characterization of the root -lesion nematode, Pratylenchus neglectus, on corn from Henan Province of China

By: Y. H. Xia,  Y. K. Liu,  P. H. Hao,  H. X. Yuan,  K. Wang,  H. L. Li and  Y. Li  
Open Access
|Dec 2021

Figures & Tables

Fig. 1

Light micrographs of Pratylenchus neglectus from corn in Henan Province of China. Females (A -M): A and M, Entire body; B: Anterior region; C-E: lip region; F: Anterior end of genital gland; G: Lateral line; H: Post-vulval region and ovary; I-L: tail region. Scale bars: 50μm (A, M) and 20μm (B-L).
Light micrographs of Pratylenchus neglectus from corn in Henan Province of China. Females (A -M): A and M, Entire body; B: Anterior region; C-E: lip region; F: Anterior end of genital gland; G: Lateral line; H: Post-vulval region and ovary; I-L: tail region. Scale bars: 50μm (A, M) and 20μm (B-L).

Fig. 2

PCR amplification of the rDNA-ITS and rDNA 28S D2-D3 expansion region of Pratylenchus neglectus. M: DL2000 Marker; 1: rDNA-ITS; 2: D2-D3 expansion region of the 28S rDNA.
PCR amplification of the rDNA-ITS and rDNA 28S D2-D3 expansion region of Pratylenchus neglectus. M: DL2000 Marker; 1: rDNA-ITS; 2: D2-D3 expansion region of the 28S rDNA.

Fig. 3

Bayesian tree of Pratylenchus as inferred from rDNA-ITS sequences under GTR+I+G model. Posterior probabilities more than 50% are given for appropriate clades. Newly obtained sequence is indicated in bold font.
Bayesian tree of Pratylenchus as inferred from rDNA-ITS sequences under GTR+I+G model. Posterior probabilities more than 50% are given for appropriate clades. Newly obtained sequence is indicated in bold font.

Fig. 4

Bayesian tree of Pratylenchus as inferred from rDNA 28S D2-D3 sequences under GTR+I+G model. Posterior probabilities more than 50% are given for appropriate clades. Newly obtained sequence is indicated in bold font.
Bayesian tree of Pratylenchus as inferred from rDNA 28S D2-D3 sequences under GTR+I+G model. Posterior probabilities more than 50% are given for appropriate clades. Newly obtained sequence is indicated in bold font.

Measurements of the Pratylenchus neglectus population morphological characteristics_

CharacterSY-XPourjam et al. (1999)Ryss (1988)
n163210
L459.0 ± 23.0(431.0 – 498.0)490(300 – 590)600(410 – 700)
a22.9 ± 1.6(20.0 – 26.1)26.7(22.3 – 32.2)24(17 – 31)
b5.7 ± 0.5(5.0 – 6.6)6.0(4.3 – 7.6)6.3(4.9 – 7.1)
b’4.3 ± 0.3(3.9 – 4.7)4.3(3.4 – 5.2)__
c18.7 ± 2.2(15.2 – 24.7)21.4(14.8 – 28.2)21(13 – 31)
c’2.1 ± 0.3(1.5 – 2.6)2.1(1.5 – 3.0)1.9(1.5 – 2.5)
V80.5 ± 2.1(77.5 – 84.6)82(80 – 85)83(77 – 85)
Stylet length15.8 ± 1.1(14.3 – 17.4)16(14 – 18)16(15.5 – 17.5)
Stylet shaft8.1 ± 0.5(7.3 – 9.3)____
Stylet knob width4.1 ± 0.4(3.2 – 4.8)____
Stylet knob height2.0 ± 0.3(1.6 – 2.7)____
DGO from stylet base2.5 ± 0.5(1.4 – 3.3)____
center of metacorpus45.7 ± 2.7(40.3 – 50.5)____
end of pharyngeal gland lobe107.0 ± 4.3(98.5 – 113.0)____
Anterior end to excretory pore74.5 ± 4.5(68.5 – 85.0)____
Pharyngeal overlap26.7 ± 4.9 (19.6 – 39.4)____
Max body diam.20.1 ± 1.6 (17.8 – 23.7)____
Vulval body diam.17.9 ± 1.2(16.0 – 20.00)____
Anal body diam.12.0 ± 1.1(10.6 – 14.8)____
Anterior genital trace length147.4 ± 21.9(102.8 – 192.7)____
Tail length24.8 ± 2.4(20.0 – 29.0)____
No. of tail annuli20.7 ± 2.3(18 – 24)____
Vulva to annus distance68.0 ± 11.5(53.5 – 96.5)____
Post- uterine sac length19.7 ± 4.2(13.0 – 21.7)____
Lateral field width7.2 ± 1.1(5.8 – 8.8)____
Lip width8.1 ± 0.4(7.1 – 8.6)____
Lip height2.4 ± 0.4(1.7 – 3.1)____
DOI: https://doi.org/10.2478/helm-2021-0044 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
Language: English
Page range: 385 - 393
Submitted on: May 1, 2021
|
Accepted on: Oct 29, 2021
|
Published on: Dec 25, 2021
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2021 Y. H. Xia, Y. K. Liu, P. H. Hao, H. X. Yuan, K. Wang, H. L. Li, Y. Li, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.