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Comparative comprehensive analysis on natural infections of Hymenolepis diminuta and Hymenolepis nana in commensal rodents Cover

Comparative comprehensive analysis on natural infections of Hymenolepis diminuta and Hymenolepis nana in commensal rodents

By: S. K. Brar,  N. Singla and  L. D. Singla  
Open Access
|Sep 2021

Figures & Tables

Fig. 1

Eggs of H. diminuta in an unstained wet mount of the faecalsample showing presence of hooks in the oncosphere and no polar filaments at 40x (a & b); Eggs of H. nana in an unstained wet mount of the faecal sample showinghooks in the oncosphere and polar filaments within the space between the oncosphere and outer shell at 40x (c & d)
Eggs of H. diminuta in an unstained wet mount of the faecalsample showing presence of hooks in the oncosphere and no polar filaments at 40x (a & b); Eggs of H. nana in an unstained wet mount of the faecal sample showinghooks in the oncosphere and polar filaments within the space between the oncosphere and outer shell at 40x (c & d)

Fig. 2

Tapeworm of H. nana showing four suckers (two visible in the view), an armed rostellum and gravid proglottids filled with eggs at 10x (a) and 40x (b); Tapeworm of H. diminuta showing four suckers, but only two visible in this view, an unarmed rostellum, mature proglottids and gravid proglottids containing a large number of eggs at 40x (c and d)
Tapeworm of H. nana showing four suckers (two visible in the view), an armed rostellum and gravid proglottids filled with eggs at 10x (a) and 40x (b); Tapeworm of H. diminuta showing four suckers, but only two visible in this view, an unarmed rostellum, mature proglottids and gravid proglottids containing a large number of eggs at 40x (c and d)

Fig. 3

Section of intestine showing parasitic enteritis (p), scolex of H. nana embedded in the mucosa (arrows), sloughing of intestinal mucosa with infiltration of mononuclear cells (mnc) and necrosis (nc) of villi at 20x (a)&40x (b); Section of intestine infected with H. nana showing severe mucous degeneration with lymphoid cell proliferation (lcp) andnecrosis in the submucosa (arrow) at 4x (c); Section of intestine infected with H. diminuta showing lymphoid cell proliferation (lcp) and eosinophilic cellular infiltration (ecf) in the submucosa at 10x (d)
Section of intestine showing parasitic enteritis (p), scolex of H. nana embedded in the mucosa (arrows), sloughing of intestinal mucosa with infiltration of mononuclear cells (mnc) and necrosis (nc) of villi at 20x (a)&40x (b); Section of intestine infected with H. nana showing severe mucous degeneration with lymphoid cell proliferation (lcp) andnecrosis in the submucosa (arrow) at 4x (c); Section of intestine infected with H. diminuta showing lymphoid cell proliferation (lcp) and eosinophilic cellular infiltration (ecf) in the submucosa at 10x (d)

Fig. 4

The PCR amplification of ITS-2 regions of the adult cestodes showing single band of H. diminuta (269 Bp) and H. nana (242 bp)
The PCR amplification of ITS-2 regions of the adult cestodes showing single band of H. diminuta (269 Bp) and H. nana (242 bp)

Fig. 5

Multiple alignment of H. diminuta (Punjab, India) with different geographical isolates.
Multiple alignment of H. diminuta (Punjab, India) with different geographical isolates.

Fig. 6

Multiple alignment of H. nana (Punjab, India) with different geographical isolates.
Multiple alignment of H. nana (Punjab, India) with different geographical isolates.

Fig. 7

Phylogenetic tree of H. diminuta (Punjab, India) constructed using Maximum Likelihood method in MEGA X software.
Phylogenetic tree of H. diminuta (Punjab, India) constructed using Maximum Likelihood method in MEGA X software.

Fig. 8

Phylogenetic tree of H. nana (Punjab, India) constructed using Maximum Likelihood method in MEGA X software.
Phylogenetic tree of H. nana (Punjab, India) constructed using Maximum Likelihood method in MEGA X software.

Infection rates of adult parasites of H_ diminuta and H_ nana in commensal rodents when found alone and in concurrence to each other_

Rodents speciesEndoparasites FoundNumber of host infectedPercent host infectedNumber of parasites foundMean intensityMean abundance
H. diminuta10652.732382.241.18
Rattus rattusH. nana12863.705754.492.86
(n=201)Concurrent infection10250.746136.013.04

H. diminuta5055.551122.241.24
bengalensis BandicotaH. nana6167.782854.673.16
(n=90)Concurrent infection4651.112655.762.94

(n=291)Overall19767.7012106.144.15

j_helm-2021-0027_tab_004

Cestode species Egg dimensions (Mean±SD)
Standard size of
Length (μm)Breadth (μm)Average
eggs (μm)
(Range)(Range)Length (μm)Breadth (μm)(Wharyet al., 2015)
H. diminuta72.75 – 80.2564.75 – 70.5076.50±3.0167.62±2.4270 – 85 x 60 – 80

H. nana45.75 – 50.0032.00 – 40.2547.87±1.9536.12±3.0540 – 60 x 30 – 50

Comparative study of H_ diminuta in two commensal rodents from different locations of Ludhiana with relation to season, age and sex of the host_

Epidemiological factorsHost examinedInfectedUn-infectedPercentage of hostsParasite numberx2 valueP value (d.f.)Odd ratioRelative risk95% Confidence interval
infected Lower limitUpper limit
SeasonsWinter96465047.911022.420.290.921.000.741.35
Summer97583959.79130 (2)1.481.260.961.65
Monsoon98524653.06118 1.131.130.851.50
LocationResidences/shops111674460.361603.460.171.521.150.901.47
Poultry farms120586248.33119 (2)0.931.000.761.29
Fish market60312951.6771 1.061.060.781.45
SpeciesR. rattus2011069552.742380.680.401.111.000.831.20
B. bengalensis90504055.55112 (1)1.251.050.841.32
SexMale126695754.761440.0160.891.211.030.831.29
Female165877852.72206 (1)1.111.000.811.23
AgeMature2381459360.9232628.12<0.0001*1.562.821.654.83
Young53114220.7524 (1)0.261.000.472.10

Overall29115613553.60350------------

Comparative study of H_ nana in two commensal rodents from different locations of Ludhiana with relation to season, age and sex of the host_

Epidemiological factorsHost examinedInfectedUn-infectedPercentage of hostsParasite numberx2 valueP value (d.f.)Odd ratioRelative risk95% Confidence interval
infected Lower limitUpper limit
SeasonsWinter96564058.332650.610.73 (2)1.401.000.781.27
Summer97702772.16306 2.591.251.001.55
Monsoon98633564.28289 1.801.120.891.41
LocationResidences/shops111813072.973680.280.86 (2)2.701.150.951.40
Poultry farms120705058.33314 1.401.000.801.23
Fish market60382263.33178 1.721.080.851.38
SpeciesR. rattus2011287363.685750.140.69 (1)1.751.000.861.15
B. bengalensis90612967.78285 2.101.060.891.26
SexMale126824465.073700.080.77 (1)1.861.010.841.19
Female1651075864.84490 1.841.000.841.17
AgeMature2381637568.497927.180.007*2.171.341.001.79
Young53262749.0668 (1)0.961.000.671.47

Overall29118910264.94860------------
DOI: https://doi.org/10.2478/helm-2021-0027 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
Language: English
Page range: 248 - 262
Submitted on: Jun 13, 2020
|
Accepted on: Mar 29, 2021
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Published on: Sep 30, 2021
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2021 S. K. Brar, N. Singla, L. D. Singla, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.