Table 1
Significant differences between Astiotrema impletum described in this study and the trematode recorded by Hamada (2002).
| Feature | Present Study | Hamada (2002) |
|---|---|---|
| Host | Fahaka, Nile Pufferfish Tetraodon lineatus Tetraodontiformes Tetradontidae | White Tilapia Orechromis niloticus Perciformes Cichlidae |
| Oral sucker much larger than ventral sucker. | yes | no |
| Ventral sucker found in the anterior quarter of the worm. | no | yes |
| Ventral sucker surrounded by numerous spines | no | yes |
| Ventral sucker with sensory papillae and tend to be in four clusters. | yes | No, papillae absent. |
| Tegumental area between the oral and ventral suckers. | Square-shaped and has relatively small spines directed posterolaterally and arranged in parallel rows. | Rectangular and naked. |
Table 2
Statistical parameters of dimensions and indices of the adult and juvenile Astiotrema impletum (Looss, 1899) Looss 1900 from the Nile puffer fish Tetraodon lineatus Linnaeus, 1758 in Egypt. (Metric characters are in mm.)
| Morphological | Adult | Juvenile | ||||||
|---|---|---|---|---|---|---|---|---|
| parameters | Mean | SD | n | SE | Mean | SD | n | SE |
| Total body length | 2 | 0.3860 | 8 | 0.1367 | 1.19 | 0.1400 | 8 | 0.0574 |
| Maximum width | 0.5 | 0.1455 | 8 | 0.0515 | 0.46 | 0.0770 | 8 | 0.0315 |
| Oral sucker length | 0.27 | 0.0489 | 8 | 0.0122 | 0.24 | 0.0200 | 8 | 0.0083 |
| Oral sucker width | 0.34 | 0.1132 | 8 | 0.0128 | 0.27 | 0.0340 | 8 | 0.0173 |
| Ventral sucker length | 0.16 | 0.0351 | 8 | 0.0132 | 0.15 | 0.0270 | 7 | 0.0097 |
| Ventral sucker width | 0.18 | 0.0348 | 8 | 0.0131 | 0.15 | 0.0320 | 7 | 0.0114 |
| Ant. testis length | 0.27 | 0.0443 | 8 | 0.0156 | 0.13 | 0.0450 | 6 | 0.0183 |
| Ant. testis width | 0.23 | 0.0512 | 8 | 0.0181 | 0.12 | 0.0400 | 6 | 0.0166 |
| Post. testis length | 0.34 | 0.0504 | 8 | 0.0178 | 0.15 | 0.0640 | 6 | 0.0264 |
| Post. testis width | 0.27 | 0.0296 | 8 | 0.0104 | 0.13 | 0.0650 | 6 | 0.0265 |
| Cirrus sac length | 0.32 | 0.0977 | 7 | 0.0369 | 0.12 | 0.0400 | 3 | 0.0233 |
| Ovary length | 0.16 | 0.0349 | 8 | 0.0123 | - | - | - | - |
| Ovary width | 0.18 | 0.0370 | 8 | 0.0130 | - | - | - | - |
| Egg length | 0.040 | 0.0055 | 8 | 0.0021 | - | - | - | - |
| Egg width | 0.0124 | 0.0028 | 8 | 0.0012 | - | - | - | - |
Fig. 1
Scanning electron micrographs (SEM) of adult Astiotrema impletum.
A – Ventral view of whole body. B – Distribution of sensory papillae around oral sucker and mouth. C – Different shapes of sensory papillae on oral sucker. D – Randomly distributed papillae on naked tegument surrounding ventral sucker. E – Solitary knob-like ciliated papillae and grouped papillae in vicinity of ventral sucker. Abbreviations: ccp – conical ciliated papillae; cnp – conical non-ciliated papillae; ecp – embedded ciliated papillae; enp – embedded non-ciliated papillae; eo – excretory opening; go – genital opening; kcp – knob-like ciliated papillae; mo – mouth opening; os – oral sucker; vs – ventral sucker; arrowheads indicate sensory papillae.
Fig. 2
SEM of ventral surface of adult A. impletum.
A – Naked area around and anterior ventral sucker. B – Tegumental spines of anterior ventro -lateral surface of body, just posterior to oral sucker. C – Tegumental spines on the ventro- lateral part, at level of ventral sucker, are long and pointed. D – Tegumental spines on the ventro -lateral part, at level of third quarter of body are smaller, fewer and more corrugated or with parallel surface ridges. E – Cytoplasmic processes on tegument of posterior end have a vermiculate, brain-like velvety appearance. F – Excretory opening is slightly shifted towards ventral surface. Abbreviations: eo – excretory opening; na – naked area in front of ventral sucker; vs – ventral sucker.
Fig. 3
SEM of juvenile Astiotrema impletum.
A – Ventral view of whole body. B – Distribution of sensory papillae on and around oral sucker. C – Genital opening. D - Ventral sucker surrounded by a number of randomly distributed sensory papillae. E – Tegumental spines on anterior ventro -lateral side of body, just posterior to oral sucker. Note pocket-like tegument covering bases of spines. F – Tegumental spines on ventro -lateral surface of body at level of ventral sucker. The spines are small in size and with parallel surface ridges or corrugated. G – Cytoplasmic processes of tegument display a fine granulated or small cobblestone-like appearance on the posterior end of body. Abbreviations: go – genital opening; mo – mouth opening; os – oral sucker; vs – ventral sucker; arrowheads indicate sensory papillae.