Fig 1
Fig 2
Fig 3
![MrBayes tree of the Xiphinema diffusum populations from GenBank and newly sequenced from Pakistan based on the 18S rDNA gene under GTR+I+G model ((-lnL = 4705.5454; AIC = 9583.090780; freqA = 0.2774; freqC = 0.2134; freqG = 0.2590; freqT = 0.2502; R(a) [AC] = 1.2052; R(b) [AG] = 2.9726; R(c) [AT] = 1.6440; R(d) [CG] = 0.4703; R(e) [CT] = 4.9113; R(f) [GT] = 1.0000; p-inv = 0.5080; gamma shape = 0.2770).](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6471e8ad215d2f6c89db4b70/j_helm-2021-0012_fig_003.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=ASIA6AP2G7AKKK2F7PAQ%2F20260221%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20260221T121817Z&X-Amz-Expires=3600&X-Amz-Security-Token=IQoJb3JpZ2luX2VjEOP%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaDGV1LWNlbnRyYWwtMSJIMEYCIQCVxvfM7B9cX9AL5RKkwvWN%2FDV6Uc%2BhB%2FeCfMVBGaU2XgIhAKQ3BTZepptDSCUmn3aBb2c8U%2FO%2FbHRQ0FJN%2BddmKrSOKsUFCKz%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEQAhoMOTYzMTM0Mjg5OTQwIgxwk3UdKxgwAthKCd4qmQVrfHz81grPYNSw40TgPMYbI2%2BirmQIwKFyH2srsZRCJ65VRjgvXbtOLWPOJkqYXOSkopOyArZyPY%2FfCAf%2BesQN7N2T%2Fy%2FdhN%2FKDMjbzzKK%2BOeY3qXAtO8yijKNXpZuhpVIZJH0%2BgdGMsHq2CW3zvXNwXI%2Fk7On7ddGWIxt%2BKDbUzw0UAkCSKqRT4kxjbdzLoMuJqgC7oX8YGKlmshj9fQxeaPuZmSl8hrOmDZQ%2FUMmiU4brBu9BVCMo8lcDsSDSP5qdBLzLI%2F2mFP2i0SQmOZk%2Bj3pQDJxX6PnlHU6AhnMhNVgB85KsQhnCLJ7hIEPE5Q29Wy3de1YSxSZ6iYtFpqCYIBrGyLiDYTBurlBxtAXMjfV4A3%2BDqhyD6bCEHDsVfZJpsws%2BpWNbfgLahtNJTV0Eimd7f8h0Ec3UXwrRDnTxbSavdvuk5BbJe2Y%2BOP1CCkNqt6tTKI5tl9pRtNZDtzhnNXIN2fdXeuTUYb02OpENJIsCm%2BJkiUKyM5ShEoopHFFQkQ6dr4DlFWcSVXhWO8YVUduFi%2FNJ6AiH5T1wD6W7MZFVe70YmBl1F%2Bbu2HIiX9AoA3xPfMuwVxj%2FoMxJUCDn2L9tkdOwolLX2ekXiDJ22QR6CEQ9fKsUTLTeVkejgfk4tCtJ1tCvpRJGKrr%2BuiN2i8OeOVCU5CdSefyBeTGAl1bbHdliuXgpYs8R0B6ZDRJozX7YEsgoW95KEy440h%2BQqSYE9kJ4YatUMQbio%2FWuV%2FClDZv2nXWk9jKebaoKYeLVIz6AkscByg4w9rUG5%2F2RP84NwLlrz2A33TQ2x34JSHb1v4wRueA2UwZh6Z0BuZBp4VKFN7xh%2FghMf0kUl90qqhZ8WsjHVBD9j2xpjjM4d%2B16SO%2FSSJFITCanObMBjqwAf%2Fh%2FpzJ0Jsd00NzE0YIPk%2Fe21QBlm4oiTxML19oJvLKHdodVr50VUs1yNzFEmnqWD3UEqj8a5hoaF%2Fb3U%2FEzCx%2Buo4OSFOEguZuslu8xGFye4NJVyPcVf6I75KtrVdi5VpZWL%2FPalMEZN8u1yW0OiByWb1vyjBUUSO%2BSh34gVQFOENSOiVKkEP%2B7mESXZOeN7mR3sZQzjxAhC5WBIKPLKTfR4RCTVUsn9YBwh253YPb&X-Amz-Signature=7f4256c08e25da150a214132a7f7633fc9eec229426fe92d74fd1a3bbeaf73e4&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject)
Morphometric of Xiphinema diffusum Lamberti & Bleve-Zacheo, 1979_ Mean ± SD (range) in μm except for L_
| Females (n = 8) | J1 (n = 1) | J2 (n = 4) | J3 (n = 1) | |
|---|---|---|---|---|
| L | 1.736 ± 103.02 (1.6 – 1.9) | (1063) | 1073 ± 33.5 | (1317) |
| a | 44.3 ± 1.8 | 40.8 | 46.1 ± 0.9 | 50.6 |
| b | 6.2 ± 0.5 | 5.0 | 4.7 ± 0.4 | 4.8 |
| c | 60.1 ± 2.5 | 44.2 | 30.2 ± 3.5 | 43.9 |
| c’ | 1.1 ± 0.1 | 1.7 | 2.3 ± 0.5 | 1.6 |
| V | 52.4 ± 0.9 | – | – | – |
| Odontostyle | 87.5 ± 1.7 | 62 | 62.2 ± 1.8 | 71 |
| Odontophore | 52.8 ± 1.1 | 42 | 37.2 ± 0.8 | 42 |
| Total stylet | 140 ± 0 | 104 | 99.5 ± 1.1 | 113 |
| Replacement odontostyle | – | 72 | 74 ± 3.1 (70 – 78) | 86 |
| Oral aperture to guiding ring | 68.3 ± 5.41 | 46 | 47.2 ± 1.9 | 57 |
| Pharyngeal bulb length | 66.8 ± 2.6 | 44 | 51.5 ± 4.0 | 56 |
| Pharyngeal bulb width | 19.7 ± 0.6 | 18 | 15.7 ± 0.4 | 18 |
| Body diameter at lip region | 11.8 ± 0.7 | 9 | 8.5 ± 0.5 | 10 |
| Body diameter at guiding ring | 27.7 ± 1.1 | 20 | 18.5 ± 0.5 | 23 |
| Body diameter at base of pharynx | 34.8 ± 1.1 | 26 | 23.5 ± 0.5 | 29 |
| Body diameter at vulva | 37.8 ± 3.4 | – | – | – |
| Body diameter at anus | 26.8 ± 1.3 | 14 | 15 ± 0.70 | 19 |
| Body diameter at beginning of J | 13.3 ± 1.1 | 6 | 5.7 ± 0.4 | 7 |
| Genital Primordium (GP %) | – | 54.5 | 53.0 ± 2.2 | 52.0 |
| Hyaline portion of tail (J) | 7.9 ± 0.2 | 6 | 7.0 ± 0.70 | 7 |
| Tail | 35.5 ± 5.5 | 24 | 29 ± 0.8 | 30 |