Fig 1
Xiphinema diffusum Lamberti & Bleve-Zacheo, 1979. A: Anterior end. B: Amphid. C: Odontostyle and odontophore. D: Vaginal region. E: Female posterior end. F: Juvenile posterior end. G: Entire female.
Fig 2
Xiphinema diffusum Lamberti & Bleve-Zacheo, 1979. A: Anterior end. B: Odontostyle. C: Pharyngeal-intestinal junction. D, E: Vaginal region. E: Female posterior end. (scale bar = 20 μm)
Table 1
Morphometric of Xiphinema diffusum Lamberti & Bleve-Zacheo, 1979. Mean ± SD (range) in μm except for L.
| Females (n = 8) | J1 (n = 1) | J2 (n = 4) | J3 (n = 1) | |
|---|---|---|---|---|
| L | 1.736 ± 103.02 (1.6 – 1.9) | (1063) | 1073 ± 33.5 (1.0 – 1.1) | (1317) |
| a | 44.3 ± 1.8 (42 – 47) | 40.8 | 46.1 ± 0.9 (45.0 – 47.2) | 50.6 |
| b | 6.2 ± 0.5 (5.7 – 7.1) | 5.0 | 4.7 ± 0.4 (4.3 – 5.3) | 4.8 |
| c | 60.1 ± 2.5 (57 – 64) | 44.2 | 30.2 ± 3.5 (28.1 – 34.8) | 43.9 |
| c’ | 1.1 ± 0.1 (0.9 – 1.2) | 1.7 | 2.3 ± 0.5 (1.9 – 3.0) | 1.6 |
| V | 52.4 ± 0.9 (52 – 54) | – | – | – |
| Odontostyle | 87.5 ± 1.7 (85 – 90) | 62 | 62.2 ± 1.8 (60 – 64) | 71 |
| Odontophore | 52.8 ± 1.1 (51 – 54) | 42 | 37.2 ± 0.8 (36 – 38) | 42 |
| Total stylet | 140 ± 0 (138 – 144) | 104 | 99.5 ± 1.1 (98 – 101) | 113 |
| Replacement odontostyle | – | 72 | 74 ± 3.1 (70 – 78) | 86 |
| Oral aperture to guiding ring | 68.3 ± 5.41 (62 – 76) | 46 | 47.2 ± 1.9 (45 – 50) | 57 |
| Pharyngeal bulb length | 66.8 ± 2.6 (64 – 79) | 44 | 51.5 ± 4.0 (46 – 57) | 56 |
| Pharyngeal bulb width | 19.7 ± 0.6 (19 – 21) | 18 | 15.7 ± 0.4 (15 – 16) | 18 |
| Body diameter at lip region | 11.8 ± 0.7 (11 – 13) | 9 | 8.5 ± 0.5 (8 – 9) | 10 |
| Body diameter at guiding ring | 27.7 ± 1.1 (26 – 29) | 20 | 18.5 ± 0.5 (18 – 19) | 23 |
| Body diameter at base of pharynx | 34.8 ± 1.1 (33 – 36) | 26 | 23.5 ± 0.5 (23 – 24) | 29 |
| Body diameter at vulva | 37.8 ± 3.4 (34 – 44) | – | – | – |
| Body diameter at anus | 26.8 ± 1.3 (25 – 28) | 14 | 15 ± 0.70 (14 – 16) | 19 |
| Body diameter at beginning of J | 13.3 ± 1.1 (12 – 15) | 6 | 5.7 ± 0.4 (5 – 6) | 7 |
| Genital Primordium (GP %) | – | 54.5 | 53.0 ± 2.2 (49.5 – 55.5) | 52.0 |
| Hyaline portion of tail (J) | 7.9 ± 0.2 (7.5 – 8.0) | 6 | 7.0 ± 0.70 (6 – 7) | 7 |
| Tail | 35.5 ± 5.5 (30 – 42) | 24 | 29 ± 0.8 (28 – 30) | 30 |
Fig 3
MrBayes tree of the Xiphinema diffusum populations from GenBank and newly sequenced from Pakistan based on the 18S rDNA gene under GTR+I+G model ((-lnL = 4705.5454; AIC = 9583.090780; freqA = 0.2774; freqC = 0.2134; freqG = 0.2590; freqT = 0.2502; R(a) [AC] = 1.2052; R(b) [AG] = 2.9726; R(c) [AT] = 1.6440; R(d) [CG] = 0.4703; R(e) [CT] = 4.9113; R(f) [GT] = 1.0000; p-inv = 0.5080; gamma shape = 0.2770).