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In-silico analysis of mt-CO1 gene of Taenia hydatigena sheep isolates Cover

In-silico analysis of mt-CO1 gene of Taenia hydatigena sheep isolates

Open Access
|Feb 2021

Figures & Tables

Fig. 1

The appearance of mt-CO1 (339 bp) haplotypes of sheep isolates of Cysticercus tenuicollis. Geographical distribution of the haplotypes is indicated by different colors. The size of the circles is related to the haplotype frequency. Number of mutations distinguishing the haplotypes is shown by hatch marks.
The appearance of mt-CO1 (339 bp) haplotypes of sheep isolates of Cysticercus tenuicollis. Geographical distribution of the haplotypes is indicated by different colors. The size of the circles is related to the haplotype frequency. Number of mutations distinguishing the haplotypes is shown by hatch marks.

Fig. 2

Phylogenetic tree view of sheep isolates using mt-CO1 gene (339 bp) sequences and reference sequences. MEGA X was used to construct a Maximum Likelihood tree based on the HKY+G model. The reliability of the tree was assessed by 1000 bootstrap replications.
Phylogenetic tree view of sheep isolates using mt-CO1 gene (339 bp) sequences and reference sequences. MEGA X was used to construct a Maximum Likelihood tree based on the HKY+G model. The reliability of the tree was assessed by 1000 bootstrap replications.

Grouping haplotypes of Cysticercus tenuicollis mt-CO1 sequences and accession numbers of isolates forming groups_

Haplotype nameNo of isolatesAccession Numbers
Hap0121KM032303-Palestine, KM032302-Palestine, KM032300-Palestine, KM032299-Palestine, KM032298-Palestine, KM032289-Palestine, KM032288-Palestine, KM032287-Palestine, KM032286-Palestine, KM032285-Palestine, KM032284-Palestine, JQ710614-Iran, JQ710599- Iran, JQ710588-Iran, KT372530-Italy, KT372529-Italy, KT372522-Italy, MH113922-Iraq, MH113921-Iraq, MH113920-Iraq, MH113919-Iraq
Hap022JQ710627-Iran, JQ710594-Iran
Hap031JQ710626-Iran
Hap041JQ710625-Iran
Hap051JQ710624-Iran
Hap061JQ710623-Iran
Hap071JQ710622-Iran
Hap081JQ710621-Iran
Hap091JQ710620-Iran
Hap101JQ710619-Iran
Hap111JQ710618-Iran
Hap122JQ710617-Iran, KM032294-Palestine
Hap131JQ710616-Iran
Hap141JQ710615-Iran
Hap151JQ710613-Iran
Hap168JQ710612-Iran, JQ710593-Iran, KT372528-Italy, KT372525-Italy, KT372524-Italy, KM032292-Palestine, KM032291-Palestine, DQ995656-India
Hap171JQ710611-Iran
Hap181JQ710610-Iran
Hap191JQ710609-Iran
Hap201JQ710608-Iran
Hap211JQ710607-Iran
Hap221JQ710606-Iran
Hap231JQ710605-Iran
Hap242JQ710604-Iran, KM032296-Palestine
Hap251JQ710603-Iran
Hap261JQ710602-Iran
Hap271JQ710601-Iran
Hap282JQ710600-Iran, JQ710591-Iran
Hap291JQ710598-Iran
Hap301JQ710597-Iran
Hap311JQ710596-Iran
Hap321JQ710595-Iran
Hap332JQ710592-Iran, KT372520-Italy
Hap341JQ710590-Iran
Hap353JQ710589-Iran, KT372518-Italy, KM032301-Palestine
Hap361KT372531-Italy
Hap371KT372527-Italy
Hap381KT372526-Italy
Hap391KT372523-Italy
Hap401KT372521-Italy
Hap411KT372517-Italy
Hap421KM032297-Palestine
Hap431KM032295-Palestine
Hap441KM032293-Palestine
Hap451KM032290-Palestine
Hap461EU544551-Finland
Hap472NC012896-China, GQ228819-China

Access numbers of the mt-CO1 gene region of C_ tenuicollis isolates used in the study_

Geographical regionNumber of isolatesAccession NumbersGenbank Submit Date
China2NC012896 and GQ2288192010 and 2016
Iran40JQ710588 - JQ710599 JQ710600 - JQ7106272012
Palestine20KM032284 - KM032299 KM032300 - KM0323032014
Italy (Sardinia)14KT372517 - KT372518 KT372520 - KT3725312015
Finland1EU5445512016
India1DQ9956562016
Iraq4MH113919 - MH1139222018

Total82

Diversity and neutrality indices for Cysticercus tenuicollis isolates from Sardinia, Iran, Palestine, Iraq, Finland, India and China using nucleotide data of mt-CO1 gene (339 bp)_

No of isolates (n)Polymorphic siteshnhd±SDπd ± SDTajima’s Dp valueFu’s Fsp value
Total (82)44470.926 ± 0.0230.008 ± 0.0009-2.29840.009*-60.5280.000

Diversity and neutrality indices for Taenia hydatigena isolates from sheep originating from various geographical regions using nucleotide data of mt-CO1 (339 bp)_

Geographical region (n)Polymorphic siteshnhd±SDnd ± SDTajima’s Dp valueFu’s Fsp value
Palestine (20)1090.705 ± 0.1110.004 ± 0.0011-1.6100.093*-4.5460.008
Iran (40)36350.992 ± 0.0080.011 ± 0.001-2.0170.031*-44.4110.000
Sardinia (14)12100.934 ± 0.0510.007 ± 0.0013-1.5010.125*-5.7750.003
DOI: https://doi.org/10.2478/helm-2021-0007 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
Language: English
Page range: 59 - 67
Submitted on: Apr 22, 2020
Accepted on: Oct 9, 2020
Published on: Feb 10, 2021
Published by: Slovak Academy of Sciences, Mathematical Institute
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2021 F. Celik, S. Gunyakti Kilinc, H. Kaya Kesik, H. Ahmed, S. Simsek, published by Slovak Academy of Sciences, Mathematical Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.