Table 1
Access numbers of the mt-CO1 gene region of C. tenuicollis isolates used in the study.
| Geographical region | Number of isolates | Accession Numbers | Genbank Submit Date |
|---|---|---|---|
| China | 2 | NC012896 and GQ228819 | 2010 and 2016 |
| Iran | 40 | JQ710588 - JQ710599 JQ710600 - JQ710627 | 2012 |
| Palestine | 20 | KM032284 - KM032299 KM032300 - KM032303 | 2014 |
| Italy (Sardinia) | 14 | KT372517 - KT372518 KT372520 - KT372531 | 2015 |
| Finland | 1 | EU544551 | 2016 |
| India | 1 | DQ995656 | 2016 |
| Iraq | 4 | MH113919 - MH113922 | 2018 |
| Total | 82 |
Table 2
Diversity and neutrality indices for Taenia hydatigena isolates from sheep originating from various geographical regions using nucleotide data of mt-CO1 (339 bp).
| Geographical region (n) | Polymorphic sites | hn | hd±SD | nd ± SD | Tajima’s D | p value | Fu’s Fs | p value |
|---|---|---|---|---|---|---|---|---|
| Palestine (20) | 10 | 9 | 0.705 ± 0.111 | 0.004 ± 0.0011 | -1.610 | 0.093* | -4.546 | 0.008 |
| Iran (40) | 36 | 35 | 0.992 ± 0.008 | 0.011 ± 0.001 | -2.017 | 0.031* | -44.411 | 0.000 |
| Sardinia (14) | 12 | 10 | 0.934 ± 0.051 | 0.007 ± 0.0013 | -1.501 | 0.125* | -5.775 | 0.003 |
Table 3
Diversity and neutrality indices for Cysticercus tenuicollis isolates from Sardinia, Iran, Palestine, Iraq, Finland, India and China using nucleotide data of mt-CO1 gene (339 bp).
| No of isolates (n) | Polymorphic sites | hn | hd±SD | πd ± SD | Tajima’s D | p value | Fu’s Fs | p value |
|---|---|---|---|---|---|---|---|---|
| Total (82) | 44 | 47 | 0.926 ± 0.023 | 0.008 ± 0.0009 | -2.2984 | 0.009* | -60.528 | 0.000 |
Table 4
Grouping haplotypes of Cysticercus tenuicollis mt-CO1 sequences and accession numbers of isolates forming groups.
| Haplotype name | No of isolates | Accession Numbers |
|---|---|---|
| Hap01 | 21 | KM032303-Palestine, KM032302-Palestine, KM032300-Palestine, KM032299-Palestine, KM032298-Palestine, KM032289-Palestine, KM032288-Palestine, KM032287-Palestine, KM032286-Palestine, KM032285-Palestine, KM032284-Palestine, JQ710614-Iran, JQ710599- Iran, JQ710588-Iran, KT372530-Italy, KT372529-Italy, KT372522-Italy, MH113922-Iraq, MH113921-Iraq, MH113920-Iraq, MH113919-Iraq |
| Hap02 | 2 | JQ710627-Iran, JQ710594-Iran |
| Hap03 | 1 | JQ710626-Iran |
| Hap04 | 1 | JQ710625-Iran |
| Hap05 | 1 | JQ710624-Iran |
| Hap06 | 1 | JQ710623-Iran |
| Hap07 | 1 | JQ710622-Iran |
| Hap08 | 1 | JQ710621-Iran |
| Hap09 | 1 | JQ710620-Iran |
| Hap10 | 1 | JQ710619-Iran |
| Hap11 | 1 | JQ710618-Iran |
| Hap12 | 2 | JQ710617-Iran, KM032294-Palestine |
| Hap13 | 1 | JQ710616-Iran |
| Hap14 | 1 | JQ710615-Iran |
| Hap15 | 1 | JQ710613-Iran |
| Hap16 | 8 | JQ710612-Iran, JQ710593-Iran, KT372528-Italy, KT372525-Italy, KT372524-Italy, KM032292-Palestine, KM032291-Palestine, DQ995656-India |
| Hap17 | 1 | JQ710611-Iran |
| Hap18 | 1 | JQ710610-Iran |
| Hap19 | 1 | JQ710609-Iran |
| Hap20 | 1 | JQ710608-Iran |
| Hap21 | 1 | JQ710607-Iran |
| Hap22 | 1 | JQ710606-Iran |
| Hap23 | 1 | JQ710605-Iran |
| Hap24 | 2 | JQ710604-Iran, KM032296-Palestine |
| Hap25 | 1 | JQ710603-Iran |
| Hap26 | 1 | JQ710602-Iran |
| Hap27 | 1 | JQ710601-Iran |
| Hap28 | 2 | JQ710600-Iran, JQ710591-Iran |
| Hap29 | 1 | JQ710598-Iran |
| Hap30 | 1 | JQ710597-Iran |
| Hap31 | 1 | JQ710596-Iran |
| Hap32 | 1 | JQ710595-Iran |
| Hap33 | 2 | JQ710592-Iran, KT372520-Italy |
| Hap34 | 1 | JQ710590-Iran |
| Hap35 | 3 | JQ710589-Iran, KT372518-Italy, KM032301-Palestine |
| Hap36 | 1 | KT372531-Italy |
| Hap37 | 1 | KT372527-Italy |
| Hap38 | 1 | KT372526-Italy |
| Hap39 | 1 | KT372523-Italy |
| Hap40 | 1 | KT372521-Italy |
| Hap41 | 1 | KT372517-Italy |
| Hap42 | 1 | KM032297-Palestine |
| Hap43 | 1 | KM032295-Palestine |
| Hap44 | 1 | KM032293-Palestine |
| Hap45 | 1 | KM032290-Palestine |
| Hap46 | 1 | EU544551-Finland |
| Hap47 | 2 | NC012896-China, GQ228819-China |
Fig. 1
The appearance of mt-CO1 (339 bp) haplotypes of sheep isolates of Cysticercus tenuicollis. Geographical distribution of the haplotypes is indicated by different colors. The size of the circles is related to the haplotype frequency. Number of mutations distinguishing the haplotypes is shown by hatch marks.
Fig. 2
Phylogenetic tree view of sheep isolates using mt-CO1 gene (339 bp) sequences and reference sequences. MEGA X was used to construct a Maximum Likelihood tree based on the HKY+G model. The reliability of the tree was assessed by 1000 bootstrap replications.