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Ultrastructure and cytochemistry of the mature spermatozoon of Khawia armeniaca (Cholodkovsky, 1915) (Caryophyllidea: Lytocestidae), a parasite of Capoeta capoeta sevangi (De Filippi, 1865) (Teleostei, Cyprinidae)

Helminthologia
Volume 57 (2020): Issue 4 (December 2020)
By:
Open Access
|Nov 2020

Figures & Tables

Fig. 1

Cross sections of the mature spermatozoon of K. armeniaca: (a) vas deferens with five different regions of the spermatozoon (I-V); (b) region I presents one axoneme and six cortical microtubules; (c) region II shows cortical microtubules which are arranged in a semi-circle under the plasma membrane; (d-g) region III or nucleated region: small diameter of anterior part of the nucleus (d), which gradually increases (e), reaches a maximum in the middle part (f), and diminishes again more posteriorly (g); (h-j) region IV shows cortical microtubules which are arranged in two opposite rows (note one single microtubule in the middle between the two opposite rows) (h), rapidly reduced volume of cytoplasm and number of cortical microtubules (i, j); (k-l) region V with one axoneme which lost its central structure (k) and undergoes disorganization into doublets (l). Ax, axoneme; Arrowheads, attachment zones; BB, basal body of the cilium; CM, cortical microtubules; D, doublets; L, lamellae; N, nucleus. Scale bars: a = 1000 nm, b-h, l = 200 nm, i = 250 nm, j, k =125 nm.
Cross sections of the mature spermatozoon of K. armeniaca: (a) vas deferens with five different regions of the spermatozoon (I-V); (b) region I presents one axoneme and six cortical microtubules; (c) region II shows cortical microtubules which are arranged in a semi-circle under the plasma membrane; (d-g) region III or nucleated region: small diameter of anterior part of the nucleus (d), which gradually increases (e), reaches a maximum in the middle part (f), and diminishes again more posteriorly (g); (h-j) region IV shows cortical microtubules which are arranged in two opposite rows (note one single microtubule in the middle between the two opposite rows) (h), rapidly reduced volume of cytoplasm and number of cortical microtubules (i, j); (k-l) region V with one axoneme which lost its central structure (k) and undergoes disorganization into doublets (l). Ax, axoneme; Arrowheads, attachment zones; BB, basal body of the cilium; CM, cortical microtubules; D, doublets; L, lamellae; N, nucleus. Scale bars: a = 1000 nm, b-h, l = 200 nm, i = 250 nm, j, k =125 nm.

Fig. 2

Granules of glycogen in the cytoplasm of the mature spermatozoon of K. armeniaca after application of Thiéry method (1967): (a) cross section of region II; (b) cross section of region III; (c) cross section of region IV; (d) longitudinal section of region II. Ax, axoneme; CM, cortical microtubules; G, glycogen; N, nucleus. Scale bars: a-c = 200 nm, d = 250 nm.
Granules of glycogen in the cytoplasm of the mature spermatozoon of K. armeniaca after application of Thiéry method (1967): (a) cross section of region II; (b) cross section of region III; (c) cross section of region IV; (d) longitudinal section of region II. Ax, axoneme; CM, cortical microtubules; G, glycogen; N, nucleus. Scale bars: a-c = 200 nm, d = 250 nm.

Fig. 3

Schematic reconstruction of the mature spermatozoon of K. armeniaca. I–V, five different regions of the mature spermatozoon; ASE, anterior spermatozoon extremity; Ax, axoneme; AZ, attachment zones; CC, central core; CM, cortical microtubules; G, glycogen; N, nucleus; PM, plasma membrane; PSE, posterior spermatozoon extremity.
Schematic reconstruction of the mature spermatozoon of K. armeniaca. I–V, five different regions of the mature spermatozoon; ASE, anterior spermatozoon extremity; Ax, axoneme; AZ, attachment zones; CC, central core; CM, cortical microtubules; G, glycogen; N, nucleus; PM, plasma membrane; PSE, posterior spermatozoon extremity.

Variation of the maximum number of cortical microtubules (CM) in the spermatozoa of the Caryophyllidea_

FamiliesGenus SpeciesCMReferences
CapingentidaeBreviscolex orientalis15Yoneva et al., 2011
CaryophyllaeidaeArchigetes sieboldi11Bruňanská et al., 2019
Caryophyllaeus laticeps15Bruňanská & Kostič, 2012
Glaridacris catostomi Świderski & Mackiewicz, 2002
Hunturella nodulosa10Yoneva et al., 2012a
Wenyonia virilis30Gamil, 2008
LytocestidaeAtractolytocestus huronensis Bruňanská et al., 2011
Caryophyllaeides fennica19Matoušková et al., 2018
Khawia armeniaca35present study
Khawia rossittensis22Matoušková et al., 2019
Khawia sinensis15Bruňanská, 2009
Lytocestus indicus25Yoneva et al., 2012b
Monobothrioides chalmersius40Arafa & Hamada, 2004
DOI: https://doi.org/10.2478/helm-2020-0040 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
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Language: English
Page range: 353 - 360
Submitted on: Feb 5, 2020
Accepted on: Jul 9, 2020
Published on: Nov 19, 2020
Published by: Slovak Academy of Sciences, Mathematical Institute
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
Cestoda,
Caryophyllidea,
spermatozoon,
ultrastructure
Related subjects:
Life sciences,
Zoology,
Ecology,
Life sciences, other,
Medicine,
Clinical medicine,
Microbiology, virology and infection epidemiology

© 2020 M. Matoušková, M. Bruňanská, J. Nebesářová, L. G. Poddubnaya, published by Slovak Academy of Sciences, Mathematical Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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