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Efficacy of Miltefosine and Artemether on infected Biomphalaria alexandrina snails with Schistosoma mansoni: immunological and histological studies

Helminthologia
Volume 57 (2020): Issue 4 (December 2020)
By:
Open Access
|Nov 2020

Figures & Tables

Fig. 1

Effect of LC25 of the miltefosine and artemether drugs on hemocytes count adult B. alexandrina snails as compared with infected snails (controls)
Effect of LC25 of the miltefosine and artemether drugs on hemocytes count adult B. alexandrina snails as compared with infected snails (controls)

Fig.2

Effect of LC25 of the miltefosine and artemether drugs on % different hemocytes types in hemolymph of adult B. alexandrina snails as compared with infected snails (controls)
Effect of LC25 of the miltefosine and artemether drugs on % different hemocytes types in hemolymph of adult B. alexandrina snails as compared with infected snails (controls)

Plate 1

A). Normal round small (non-differentiated) cells B). Granulocyte and C). Hyalinocyte of B. alexandrina adult snails (×40). S: Round small cell, G: Granulocyte, C: Cytoplasm, DM: Double membrane, GR: Granules, N: Nucleus, (H): Hyalinocyte.
A). Normal round small (non-differentiated) cells B). Granulocyte and C). Hyalinocyte of B. alexandrina adult snails (×40). S: Round small cell, G: Granulocyte, C: Cytoplasm, DM: Double membrane, GR: Granules, N: Nucleus, (H): Hyalinocyte.

Plate 2

The morphological effect of miltefosine LC25 on different types of hemocytes in the haemolymph of treated-infected B. alexandrina snails. A): Round small non-differentiated cell, B): Granulocyte and C): Hyalinocyte (x40). S: Round small cell, G: Granulocyte, GR: Granules, N: Nucleus, (H): Hyalinocyte.
The morphological effect of miltefosine LC25 on different types of hemocytes in the haemolymph of treated-infected B. alexandrina snails. A): Round small non-differentiated cell, B): Granulocyte and C): Hyalinocyte (x40). S: Round small cell, G: Granulocyte, GR: Granules, N: Nucleus, (H): Hyalinocyte.

Plate 3

The morphological effect of artemether LC25 on different types of hemocytes in the haemolymph of treated B. alexandrina snails. A): Round small (non-differentiated), B): Granulocyte and C): Hyalinocyte (x40). S: Round small cell, G: Granulocyte, C: Cytoplasm, GR: Granules, N: Nucleus, (H): Hyalinocyte.
The morphological effect of artemether LC25 on different types of hemocytes in the haemolymph of treated B. alexandrina snails. A): Round small (non-differentiated), B): Granulocyte and C): Hyalinocyte (x40). S: Round small cell, G: Granulocyte, C: Cytoplasm, GR: Granules, N: Nucleus, (H): Hyalinocyte.

Plate 4

TEM micrographs showing normal cells of B. alexandrina snails, A). Round small non- differentiated cell, B). Granulocyte and C). Hyalinocyte. CH: chromatin, CM: cell membrane, CY: Cytoplasm, N: nucleus.
TEM micrographs showing normal cells of B. alexandrina snails, A). Round small non- differentiated cell, B). Granulocyte and C). Hyalinocyte. CH: chromatin, CM: cell membrane, CY: Cytoplasm, N: nucleus.

Plate 5

TEM micrographs showing the effect of miltefosine LC25 on hemocytes A). Round small (non- differentiated) cell, B). Granulocyte and C). Hyalinocyte of treated-infected B. alexandrina snails. CH: chromatin, CM: cell membrane, CY: Cytoplasm, GR: granules, N: nucleus, V: vacuole, PL: phagolysosome.
TEM micrographs showing the effect of miltefosine LC25 on hemocytes A). Round small (non- differentiated) cell, B). Granulocyte and C). Hyalinocyte of treated-infected B. alexandrina snails. CH: chromatin, CM: cell membrane, CY: Cytoplasm, GR: granules, N: nucleus, V: vacuole, PL: phagolysosome.

Plate 6

TEM micrographs showing the effect of LC25 artemether drug on hemocytes A). Round small (non- differentiated) cell, B). Granulocyte and C). Hyalinocyte of treated-infected B. alexandrina snails, CH: chromatin, CM: cell membrane, CY: cytoplasm, GR: granules, V: vacuole, N: nucleus, PL: phagolysosome, PP: pseudopodia.
TEM micrographs showing the effect of LC25 artemether drug on hemocytes A). Round small (non- differentiated) cell, B). Granulocyte and C). Hyalinocyte of treated-infected B. alexandrina snails, CH: chromatin, CM: cell membrane, CY: cytoplasm, GR: granules, V: vacuole, N: nucleus, PL: phagolysosome, PP: pseudopodia.

Molluscicidal activity of miltefosine and artemether against adult B_ alexandrina snails (24 hours exposure)_

Tested drugLC0LC10LC25LC50LC90Slope
PpmPpmppmppmppm
Miltefosine1.383.168.2013.8024.401.60
Artemether1.685.7911.0416.8827.971.75
DOI: https://doi.org/10.2478/helm-2020-0037 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
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Language: English
Page range: 335 - 343
Submitted on: May 8, 2020
Accepted on: Jul 8, 2020
Published on: Nov 19, 2020
Published by: Slovak Academy of Sciences, Mathematical Institute
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
miltefosine,
artemether,
hemocytes,
immunological and histological studies,
light and electron microscope
Related subjects:
Life sciences,
Zoology,
Ecology,
Life sciences, other,
Medicine,
Clinical medicine,
Microbiology, virology and infection epidemiology

© 2020 H. H. Abdel-Azeem, G. Y. Osman, M. F. El Garhy, K. S. Al Benasy, published by Slovak Academy of Sciences, Mathematical Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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