Fig. 1
An ethidium bromide-stained agarose gel (1.5 %) showing the PCR amplification product of the ITSI gene of E. granulosus. Lane M: a 100-bp plus molecular size marker; lane –ve: negative control; lane 1: camel host DNA; lane 2: cattle host DNA; lane 3: sheep host DNA; lane 4: donkey host DNA; and lane 5: rabbit host DNA.
Fig. 2
Genetic relationship between an E. canadensis isolate from a camel HC (accession no.: MK460268) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Fig. 3
Genetic relationship between an E. canadensis isolate from a camel HC (accession no.: MK460269) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Fig. 4
Genetic relationship between an E. canadensis isolate from a camel HC (accession no.: MK460272) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Table 1
Echinococcus species and GenBank accession numbers of the ITS1 gene used for phylogenetic analysis.
| HC isolates | E. granulosus | Genotype of isolate | GenBank accession no. of isolate | Reference accession no. in GenBank |
|---|---|---|---|---|
| Camel | E. canadensis | G6 | MK460268 | AJ237775.1 |
| Camel | E. canadensis | G6 | MK460269 | AJ237775.1 |
| Camel | E. canadensis | G6 | MK460272 | KP866147.1 |
| Cattle | E. granulosus s.s | G1 | MK460273 | AY969044.1 |
| Sheep | E. granulosus s.s | G1 | MK460270 | KJ363926.1 |
| Donkey | E. equinus | G4 | MK460266 | AJ237773.1 |
| Rabbit | E. canadensis | G6 | MK460267 | AJ237775.1 |
| Rabbit | E. canadensis | G6 | MK460271 | KP866147.1 |
Fig. 5
Genetic relationship between an E. granulosus s. s. isolate from a cattle HC (accession no.: MK460273) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Fig. 6
Genetic relationship between an E. granulosus s. s. isolate from a sheep HC (accession no.: MK460270) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Fig. 7
Genetic relationship between E. equinus isolates from donkeys (accession no.: MK460266) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Fig. 8
Genetic relationship between an E. canadensis isolate from HCs of experimentally infected rabbits (accession no.: MK460267) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Fig. 9
Genetic relationship between an E. canadensis isolate from HCs of experimentally infected rabbits (accession no.: MK460271) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Fig. 10
Position of nucleotide substitution in the rabbit HC partial ITS1 nucleotide sequence. GRS: GenBank reference sequence; CHC: camel HC; RHC: rabbit HC.