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Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt Cover

Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt

Helminthologia
Volume 57 (2020): Issue 2 (June 2020)
By: O.A. Mahdy,  W. M. Mousa,  S. Z. Abdel-Maogood,  S. M. Nader and  S. Abdel-Radi  
Open Access
|May 2020

Figures & Tables

Fig. 1

A – B. Toxocara vitulorum collected from calves. B) Anterior end of worm showed lips (short white arrow), posterior end of male showed coiled tail (black arrow), and posterior end of female worm showed a straight tail end (long white arrow). D) Anterior end of T.vitulorum showed three lips (lip), F) posterior end of female showed short tail (st), G) posterior end of male showed two spicules (sp), C) Toxocara canis collected from street dogs; showed the coiled tail-end of male (black arrow), the straight tail-end of female (white arrow), E) Anterior end of T. canis showed the cephalic alae and lips, F) posterior end of male showed spicules.
A – B. Toxocara vitulorum collected from calves. B) Anterior end of worm showed lips (short white arrow), posterior end of male showed coiled tail (black arrow), and posterior end of female worm showed a straight tail end (long white arrow). D) Anterior end of T.vitulorum showed three lips (lip), F) posterior end of female showed short tail (st), G) posterior end of male showed two spicules (sp), C) Toxocara canis collected from street dogs; showed the coiled tail-end of male (black arrow), the straight tail-end of female (white arrow), E) Anterior end of T. canis showed the cephalic alae and lips, F) posterior end of male showed spicules.

Plate 1

Results of agarose gel electrophoresis of the PCR products of the selected genes in Toxocara spp. showing the M. W. of each gene band.
Results of agarose gel electrophoresis of the PCR products of the selected genes in Toxocara spp. showing the M. W. of each gene band.

Plate 2

Phylogenetic tree constructed in MEGA by neighbor- joining method for the ATPase-6 region of the tested Toxocara spp.
Phylogenetic tree constructed in MEGA by neighbor- joining method for the ATPase-6 region of the tested Toxocara spp.

Plate 3

Phylogenetic tree constructed in MEGA by neighbor-joining method for the 12S region of the tested Toxocara spp.
Phylogenetic tree constructed in MEGA by neighbor-joining method for the 12S region of the tested Toxocara spp.

Plate 4

Phylogenetic tree constructed in MEGA by neighbor- joining method for the ITS-2 region of the tested Toxocara spp.
Phylogenetic tree constructed in MEGA by neighbor- joining method for the ITS-2 region of the tested Toxocara spp.

Characterization of the PCR products of the selected genes in the tested Toxocara spp_ showing the M_ W_ of the bands for each gene_

GeneM.W. (bp)
T. canisT. vitulorum from cattleT. vitulorum from buffalo
ATPase-6804828828
12S572576572
ITS-2592625625
DOI: https://doi.org/10.2478/helm-2020-0013 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
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Language: English
Page range: 83 - 90
Submitted on: May 20, 2019
|
Accepted on: Dec 21, 2019
|
Published on: May 23, 2020
Published by: Slovak Academy of Sciences, Institute of Parasitology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
molecular characterization,
PCR-mitochondrial gene- ribosomal gene,
dogs,
cattle,
buffalo,
Egypt
Related subjects:
Life sciences,
Zoology,
Ecology,
Life sciences, other,
Medicine,
Clinical medicine,
Microbiology, virology and infection epidemiology

© 2020 O.A. Mahdy, W. M. Mousa, S. Z. Abdel-Maogood, S. M. Nader, S. Abdel-Radi, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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