The influence of chestnut wood and flubendazole on morphology of small intestine and lymphocytes of peripheral blood, spleen and jejunum in broiler chickens

WBC	C	Far	Fli	Far+Fli
Leukocytes	13.72 ± 2.71	8.85 ± 2.92^a	14.58 ± 3.02^b	14.40 ± 3.46^b
Lymphocytes	6.68 ± 0.35	5.44 ± 1.57^a	8.63 ± 1.53^d	8.37 ± 0.72^d
Heterophiles	6.23 ± 2.44	2.65 ± 1.41	4.91 ± 2.39	4.63 ± 3.18
Eosinophiles	0.53 ± 0.23	0.47 ± 0.19	0.61 ± 0.34	0.88 ± 0.34
Monocytes	0.43 ± 0.06	0.29 ± 0.07^a	0.44 ± 0.14	0.46 ± 0.12^b

[i] Specific superscripts in row indicate significant differences – ^abP<0.05; ^adP<0.001

Table 2

Subpopulations of lymphocytes in the peripheral blood (total counts = G.L^–1) and spleen (relative percentage)(mean ± SD).

Peripheral blood
Groups	Subpopulations of lymphocytes (mean ± SD)
	CD3	CD4	CD8	IgM	IgA	CD45
C	2.33 ± 0.79	1.32 ± 0.44	0.80 ± 0.20	0.85 ± 0.24	0.29 ± 0.26	4.32 ± 0.62
Far	2.37 ± 1.08	1.45 ± 0.63	0.72 ± 0.37	0.58 ± 0.23^a	0.13 ± 0.11^c	2.89 ± 1.01^a
Fli	3.12 ± 0.90	1.82 ± 0.71	0.84 ± 0.19	1.20 ± 0.32^c	0.70 ± 0.39^a	5.42 ± 1.05^d
Far+Fli	3.05 ± 0.73	1.99 ± 0.69	0.75 ± 0.18	0.95 ± 0.22	0.26 ± 0.12^c	5.18 ± 0.84^d

	Spleen
C	66.14 ± 10.02	16.39 ± 9.27	39.83 ± 7.94	14.88 ± 5.64^a	25.13 ± 17.25	29.53 ± 9.67
Far	66.29 ± 6.21	24.33 ± 7.24	39.72 ± 10.82	19.91 ± 6.99	16.87 ± 5.95	30.30 ± 5.82
Fli	66.65 ± 5.92	22.59 ± 6.08	39.79 ± 5.62	26.57 ± 7.80^b	19.97 ± 2.59	32.22 ± 5.90
Far+Fli	63.51 ± 11.10	19.51 ± 8.31	35.17 ± 13.07	18.26 ± 5.67	15.24 ± 3.42	32.76 ± 8.65

[i] Specific superscripts in columns indicate significant differences – ^abP<0.05; ^acP<0.01; ^adP<0.001

Table 3

Subpopulations of lymphocytes in jejunal mucosa (mm²).

Subpopulations	C	Far	Fli	Far+Fli
CD3	598.90 ± 204.20^d	312.00 ± 105.10^a	680.10 ± 186.80^d	672.30 ± 252.00^d
CD4	614.40 ± 187.00^d	282.00 ± 94.69^a	641.70 ± 185.40^da	547.10 ± 151.70^dc
CD8	770.10 ± 213.40^d	388.60 ± 159.50^a	794.20 ± 257.30^d	743.20 ± 220.20^d
IgM	203.90 ± 122.30^a	146.70 ± 91.80^c	216.00 ± 88.14^a	149.60 ± 73.22^bc
IgA	331.10 ± 162.40	250.80 ± 180.40	294.10 ± 200.20	274.60 ± 160.40

[i] Specific superscripts in row indicate significant differences – ^abP<0.05; ^acP<0.01; ^adP<0.001

Height of duodenal villi (mean±SD, ^adP>0.001).

Cutting surface of duodenal villi (mean±SD, ^abP>0.05, ^adP>0.001).

Height of jejunal villi (mean±SD, ^abP<0.05,^*adP<0.001).

Cutting surface of jejunal villi (mean±SD, ^*abP<0.05, ^adP<0.001).

Histological pictures from duodenum of different chicken’ groups (HE stain).

Histological pictures from jejunum of different chicken’ groups (HE stain).

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DOI: https://doi.org/10.2478/helm-2019-0029 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605

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Language: English

Page range: 273 - 281

Submitted on: Jan 31, 2019

Accepted on: Aug 25, 2019

Published on: Nov 6, 2019

Published by: Slovak Academy of Sciences, Institute of Parasitology

In partnership with: Paradigm Publishing Services

Publication frequency: Volume open

Keywords:

chicken,

Related subjects:

Zoology,

Ecology,

Life sciences, other,

Medicine,

Clinical medicine,

Microbiology, virology and infection epidemiology

© 2019 M. Levkut, V. Revajová, M. Levkutová, E. Selecká, Z. Ševčíková, V. Karaffová, M. Levkut, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 56 (2019): Issue 4 (December 2019)

The influence of chestnut wood and flubendazole on morphology of small intestine and lymphocytes of peripheral blood, spleen and jejunum in broiler chickens

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