Modulation of TLR2 and TLR4 in macrophages following Trichinella spiralis infection

C. Han; J. Xu; Ch. Liu; X. Li; P. Zhai; A. Hashan; M. Song

doi:10.2478/helm-2018-0015

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Modulation of TLR2 and TLR4 in macrophages following Trichinella spiralis infection

Helminthologia

Volume 55 (2018): Issue 3 (September 2018)

By: C. Han, J. Xu, Ch. Liu, X. Li, P. Zhai, A. Hashan and M. Song

Open Access

|Jul 2018

Abstract

Parasitic helminthes can suppress and/or regulate the host immune response to allow long-term survival and chronic infection where toll-like receptors (TLRs) expressed on macrophages play essential roles in response to parasitic infection. Semi-quantitative PCR and flow cytometry studies about the modulation of TLRs and cytokine profiles in macrophages following T. spiralis infection were performed. TLRs, MyD88 and NF-κB were up-regulated by T. spiralis infection and essential to the parasite life cycles. Cytokines profiles (IL-6, IL-10, IL-12, TNF-α) were modulated during T. spiralis infection. Results suggest that T. spiralis infection may regulate the expression of TLR4 on macrophages and TLR4/MyD88/NF-κB signaling pathways. This study provides further insights into the mechanisms of TLR-mediated post-inflammatory response during T. spiralis infection.

References

Aranzamendi, C., de Bruin, A., Kuiper, R., Boog, C.J., van Eden, W., Rutten, V., Pinelli, E. (2013): Protection against allergic airway inflammation during the chronic and acute phases of Trichinella spiralis infection. Clin. Exp. Allergy, 43(1): 103 – 115. DOI: 10.1111/cea.1204210.1111/cea.1204223278885
Open DOI Search in Google Scholar Back to article
Aranzamendi, C., Fransen, F., Langelaar, M., Franssen, F., van der Ley, P., van Putten, J.P., Rutten, V., Pinelli, E. (2012): Trichinella spiralis-secreted products modulate DC functionality and expand regulatory T cells in vitro. Parasite Immunol., 34(4): 210 – 223. DOI: 10.1111/j.1365-3024.2012.01353.x10.1111/j.1365-3024.2012.01353.x22224925
Open DOI Search in Google Scholar Back to article
Bai, X., Wu, X., Wang, X., Guan, Z., Gao, F., Yu, J., Yu, L., Tang, B., Liu, X., Song, Y., Wang, X., Radu, B., Boireau, P., Wang, F., Liu, M. (2012): Regulation of cytokine expression in murine macrophages stimulated by excretory/secretory products from Trichinella spiralis in vitro. Mol. Cell. Biochem., 360(1 – 2): 79 – 88. DOI: 10.1007/s11010-011-1046-410.1007/s11010-011-1046-421909996
Open DOI Search in Google Scholar Back to article
Beiting, D.P., Gagliardo, L.F., Hesse, M., Bliss, S.K., Meskill, D., Appleton, J.A. (2007): Coordinated control of immunity to muscle stage Trichinella spiralis by IL-10, regulatory T cells, and TGF-beta. J. Immunol., 178(2): 1039 – 104710.4049/jimmunol.178.2.103917202367
Search in Google Scholar Back to article
Blum, L.K., Mohanan, S., Fabre, M.V., Yafawi, R.E., Appleton, J.A. (2013): Intestinal infection with Trichinella spiralis induces distinct, regional immune responses. Vet. Parasitol., 194(2 – 4): 101 – 105. DOI: 10.1016/j.vetpar.2013.01.03010.1016/j.vetpar.2013.01.030365512923465441
Open DOI Search in Google Scholar Back to article
Bruschi. F., Chiumiento, L. (2011): Trichinella inflammatory myopathy: host or parasite strategy? Parasit. Vectors, 4: 42. DOI: 10.1186/1756-3305-4-4210.1186/1756-3305-4-42307968421429196
Open DOI Search in Google Scholar Back to article
Bruschi, F., Korenaga, M., Watanabe, N. (2008): Eosinophils and Trichinella infection: toxic for the parasite and the host? Trends Parasitol., 24(10): 462 – 467. DOI: 10.1016/j.pt.2008.07.00110.1016/j.pt.2008.07.00118722811
Search in Google Scholar Back to article
Chen, X., Yang, Y., Yang, J., Zhang, Z., Zhu, X. (2012): RNAi-mediated silencing of paramyosin expression in Trichinella spiralis results in impaired viability of the parasite. PLoS One, 7(11): e49913. DOI: 10.1371/journal.pone.004991310.1371/journal.pone.0049913350383223185483
Search in Google Scholar Back to article
Cui, J., Ren, H.J., Liu, R.D., Wang, L., Zhang, Z.F., Wang, Z.Q. (2013):Phage-displayed specific polypeptide antigens induce significant protective immunity against Trichinella spiralis infection in BALB/c mice. Vaccine, 31(8): 1171 – 1177. DOI: 10.1016/j.vaccine.2012.12.07010.1016/j.vaccine.2012.12.07023306358
Search in Google Scholar Back to article
Du, L., Liu, L., Yu, Y., Shan, H., Li, L. (2014): Trichinella spiralis excretory-secretory products protect against polymicrobial sepsis by suppressing MyD88 via mannose receptor. Biomed. Res. Int., 2014: 898646. DOI: 10.1155/2014/89864610.1155/2014/898646409862125054155
Search in Google Scholar Back to article
Gruden-Movsesijan, A., Ilic, N., Colic, M., Majstorovic, I., Vasilev, S., Radovic, I., Sofronic-Milosavljevic, L. (2011): The impact of Trichinella spiralis excretory-secretory products on dendritic cells. Comp. Immunol. Microbiol. Infect. Dis., 34(5): 429 – 439. DOI: 10.1016/j.cimid.2011.08.00410.1016/j.cimid.2011.08.00421903269
Open DOI Search in Google Scholar Back to article
Gruden-Movsesijan, A., Ilic, N., Mostarica-Stojkovic, M., Stosic-Grujicic, S., Milic, M., Sofronic-Milosavljevic, L. (2010): Mechanisms of modulation of experimental autoimmune encephalomyelitis by chronic Trichinella spiralis infection in Dark Agouti rats. Parasite Immunol., 32(6): 450 – 459. DOI: 10.1111/j.1365-3024.2010.01207.x10.1111/j.1365-3024.2010.01207.x20500676
Open DOI Search in Google Scholar Back to article
Ilic, N., Gruden-Movsesijan, A., Sofronic-Milosavljevic, L. (2012): Trichinella spiralis: shaping the immune response. Immunol. Res., 52(1 – 2): 111 – 119. DOI: 10.1007/s12026-012-8287-510.1007/s12026-012-8287-522392054
Open DOI Search in Google Scholar Back to article
Ilic, N., Worthington, J.J., Gruden-Movsesijan, A., Travis, M.A., Sofronic-Milosavljevic, L., Grencis, R.K. (2011): Trichinella spiralis antigens prime mixed Th1/Th2 response but do not induce de novo generation of Foxp3+ T cells in vitro. Parasite Immunol., 33(10): 572 – 582. DOI: 10.1111/j.1365-3024.2011.01322.x10.1111/j.1365-3024.2011.01322.x348566921793858
Search in Google Scholar Back to article
Kang, S.A., Cho, M.K., Park, M.K., Kim, D.H., Hong, Y.C., Lee, Y.S., Cha, H.J., Ock, M.S., Yu, H.S. (2012): Alteration of helper T-cell related cytokine production in splenocytes during Trichinella spiralis infection. Vet. Parasitol., 186(3 – 4): 319 – 327. DOI: 10.1016/j.vetpar.2011.12.00210.1016/j.vetpar.2011.12.00222222009
Open DOI Search in Google Scholar Back to article
Kim, S., Park, M.K., Yu, H.S. (2015): Toll-Like Receptor Gene Expression during Trichinella spiralis Infection. Korean J. Parasitol., 53(4): 431 – 438. DOI: 10.3347/kjp.2015.53.4.43110.3347/kjp.2015.53.4.431456650126323841
Open DOI Search in Google Scholar Back to article
Langelaar, M., Aranzamendi, C., Franssen, F., Van Der Giessen, J., Rutten, V., van der Ley, P., Pinelli, E. (2009): Suppression of dendritic cell maturation by Trichinella spiralis excretory/secretory products. Parasite Immunol., 31(10): 641 – 645. DOI: 10.1111/j.1365-3024.2009.01136.x10.1111/j.1365-3024.2009.01136.x19751476
Search in Google Scholar Back to article
Lawrence, C.E., Paterson, J.C., Wei, X.Q., Liew, F.Y., Garside, P., Kennedy, M.W. (2000): Nitric oxide mediates intestinal pathology but not immune expulsion during Trichinella spiralis infection in mice. J. Immunol., 164(8): 4229 – 423410.4049/jimmunol.164.8.422910754319
Search in Google Scholar Back to article
Li, X., Liu, W., Wang, J., Zou, D., Wang, X., Yang, Z., Yin, Z., Cui, Q., Shang, W., Li, H., Wei, X., Cui, J., Wang, Z., Huang, L., Yuan, J. (2012): Rapid detection of Trichinella spiralis larvae in muscles by loop-mediated isothermal amplification. Int. J. Parasitol., 42(13 – 14): 1119 – 1126. DOI: 10.1016/j.ijpara.2012.09.01110.1016/j.ijpara.2012.09.01123146927
Open DOI Search in Google Scholar Back to article
Liu, A.Y., Dwyer, D.F., Jones, T.G., Bankova, L.G., Shen, S., Katz, H.R., Austen, K.F., Gurish, M.F. (2013): Mast cells recruited to mesenteric lymph nodes during helminth infection remain hypogranular and produce IL-4 and IL-6. J. Immunol., 190(4): 1758 – 1766. DOI: 10.4049/jimmunol.120256710.4049/jimmunol.1202567356383723319739
Search in Google Scholar Back to article
Mido, S., Fath, E.M., Farid, A.S., Nonaka, N., Oku, Y., Horii, Y. (2012): Trichinella spiralis: infection changes serum paraoxonase-1 levels, lipid profile, and oxidative status in rats. Exp. Parasitol., 131(2): 190 – 194. DOI: 10.1016/j.exppara.2012.03.02310.1016/j.exppara.2012.03.02322490818
Open DOI Search in Google Scholar Back to article
Moreau, E., Chauvin, A. (2010): Immunity against helminths: interactions with the host and the intercurrent infections. J. Biomed. Biotechnol., 2010: 428593. DOI: 10.1155/2010/42859310.1155/2010/428593281755820150967
Open DOI Search in Google Scholar Back to article
Motran, C.C., Ambrosio, L.F., Volpini, X., Celias, D.P., Cervi, L. (2017): Dendritic cells and parasites: from recognition and activation to immune response instruction. Semin. Immunopathol., 39(2): 199 – 213. DOI: 10.1007/s00281-016-0588-710.1007/s00281-016-0588-727587063
Open DOI Search in Google Scholar Back to article
Noel, W., Raes, G., Hassanzadeh Ghassabeh, G., De Baetselier, P., Beschin, A. (2004): Alternatively activated macrophages during parasite infections. Trends Parasitol., 20(3): 126 – 133. DOI: 10.1016/j.pt.2004.01.00410.1016/j.pt.2004.01.00415036034
Open DOI Search in Google Scholar Back to article
Pulendran, B., Tang, H., Manicassamy, S. (2010): Programming dendritic cells to induce T(H)2 and tolerogenic responses. Nat. Immunol., 11(8): 647 – 655. DOI: 10.1038/ni.189410.1038/ni.189420644570
Search in Google Scholar Back to article
Puneet, P., McGrath, M.A., Tay, H.K., Al-Riyami, L., Rzepecka, J., Moochhala, S.M., Pervaiz, S., Harnett, M.M., Harnett, W., Melendez, A.J. (2011): The helminth product ES-62 protects against septic shock via Toll-like receptor 4-dependent autophagosomal degradation of the adaptor MyD88. Nat. Immunol., 12(4): 344 – 351. DOI: 10.1038/ni.200410.1038/ni.200421358639
Open DOI Search in Google Scholar Back to article
Radovic, I., Gruden-Movsesijan, A., Ilic, N., Cvetkovic, J., Mojsilovic, S., Devic, M., Sofronic-Milosavljevic, L. (2015): Immunomodulatory effects of Trichinella spiralis-derived excretory-secretory antigens. Immunol. Res., 61(3): 312 – 325. DOI: 10.1007/s12026-015-8626-410.1007/s12026-015-8626-425616617
Open DOI Search in Google Scholar Back to article
Riva, E., Steffan, P., Guzman, M., Fiel, C. (2012): Persistence of Trichinella spiralis muscle larvae in natural decaying mice. Parasitol. Res., 111(1): 249 – 255. DOI: 10.1007/s00436-012-2826-910.1007/s00436-012-2826-922281547
Open DOI Search in Google Scholar Back to article
Scalfone, L.K., Nel, H.J., Gagliardo, L.F., Cameron, J.L., Al-Shokri, S., Leifer, C.A., Fallon, P.G., Appleton, J.A. (2013): Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis. Infect. Immun., 81(4): 1354 – 1363. DOI: 10.1128/IAI.01307-1210.1128/IAI.01307-12363959623403558
Open DOI Search in Google Scholar Back to article
Thrasher, S.M., Scalfone, L.K., Holowka, D., Appleton, J.A. (2013): In vitro modelling of rat mucosal mast cell function in Trichinella spiralis infection. Parasite Immunol., 35(1): 21 – 31.10.1111/pim.1201410.1111/pim.12014
Search in Google Scholar Back to article
Yadav, A.K., Temjenmongla (2012): Efficacy of Lasia spinosa leaf extract in treating mice infected with Trichinella spiralis. Parasitol. Res., 110(1): 493 – 498. DOI: 10.1007/s00436-011-2551-910.1007/s00436-011-2551-921748345
Open DOI Search in Google Scholar Back to article
Yang, J., Gu, Y., Yang, Y., Wei, J., Wang, S., Cui, S., Pan, J., Li, Q., Zhu, X. (2010): Trichinella spiralis: immune response and protective immunity elicited by recombinant paramyosin formulated with different adjuvants. Exp. Parasitol., 124(4): 403 – 408. DOI: 10.1016/j.exppara.2009.12.01010.1016/j.exppara.2009.12.01020045697
Open DOI Search in Google Scholar Back to article
Yu, Y.R., Deng, M.J., Lu, W.W., Jia, M.Z., Wu, W., Qi, Y.F. (2013): Systemic cytokine profiles and splenic toll-like receptor expression during Trichinella spiralis infection. Exp. Parasitol., 134(1): 92 – 101. DOI: 10.1016/j.exppara.2013.02.01410.1016/j.exppara.2013.02.01423466989
Open DOI Search in Google Scholar Back to article