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Polyethersulfone (PES) Membrane on Agar Plates as a Plant Growth Platform for Spaceflight

Gravitational and Space Research
Volume 10 (2022): Issue 1 (January 2022)
By:
Open Access
|Dec 2022

Figures & Tables

Figure 1

Setup for APEx-07. (A) Plate components from left to right: agar plate, PES membrane, Arabidopsis seed with guar gum, micropore tape. (B) Plates growing vertically in rack.
Setup for APEx-07. (A) Plate components from left to right: agar plate, PES membrane, Arabidopsis seed with guar gum, micropore tape. (B) Plates growing vertically in rack.

Figure 2

12-day growth comparison between black and white PES membrane. The more cost-effective white membranes showed comparable growth to black membrane.
12-day growth comparison between black and white PES membrane. The more cost-effective white membranes showed comparable growth to black membrane.

Figure 3

Harvest strategy to maintain intact seedlings. (A) Representative 12-day seedling growth in plate, (B) membrane collected with forceps, (C) membrane transferred to lid of Petri dish and wrapped in foil for freezing and shipping, and (D) a frozen plate returned from low Earth orbit.
Harvest strategy to maintain intact seedlings. (A) Representative 12-day seedling growth in plate, (B) membrane collected with forceps, (C) membrane transferred to lid of Petri dish and wrapped in foil for freezing and shipping, and (D) a frozen plate returned from low Earth orbit.

Figure 4

Ground control Veggie unit at Kennedy Space Center (left) and both Veggie units at capacity aboard the ISS (right).
Ground control Veggie unit at Kennedy Space Center (left) and both Veggie units at capacity aboard the ISS (right).

Success criteria of EVT samples as measured across 60 plates_

Success CriteriaGoalObservedScore
Germination>90%99%Excellent
radical emergence
Growth90%>99%Excellent
roots > 4cm
Contamination<10%0%Excellent
Bacterial or fungal growth
Seedling intactness>90%100%Excellent
Dissectible into roots/shoots
RNA quality>75%100%Excellent
RIN> 8, 260/280>2
DOI: https://doi.org/10.2478/gsr-2022-0004 | Journal eISSN: 2332-7774
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Language: English
Page range: 30 - 36
Published on: Dec 31, 2022
Published by: American Society for Gravitational and Space Research
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
Spaceflight,
advanced plant habitat,
microgravity
Related subjects:
Life sciences,
Life sciences, other,
Materials sciences,
Materials sciences, other,
Physics,
Physics, other

© 2022 Alexander Meyers, Eric Land, Imara Perera, Emma Canaday, Sarah E. Wyatt, published by American Society for Gravitational and Space Research
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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