Polyethersulfone (PES) Membrane on Agar Plates as a Plant Growth Platform for Spaceflight

Alexander Meyers; Eric Land; Imara Perera; Emma Canaday; Sarah E. Wyatt

doi:10.2478/gsr-2022-0004

.blurhash-client-img { display: none !important; }

Polyethersulfone (PES) Membrane on Agar Plates as a Plant Growth Platform for Spaceflight

Gravitational and Space Research

Volume 10 (2022): Issue 1 (January 2022)

By: Alexander Meyers, Eric Land, Imara Perera, Emma Canaday and Sarah E. Wyatt

Open Access

|Dec 2022

Figures & Tables

Setup for APEx-07. (A) Plate components from left to right: agar plate, PES membrane, Arabidopsis seed with guar gum, micropore tape. (B) Plates growing vertically in rack.

12-day growth comparison between black and white PES membrane. The more cost-effective white membranes showed comparable growth to black membrane.

Harvest strategy to maintain intact seedlings. (A) Representative 12-day seedling growth in plate, (B) membrane collected with forceps, (C) membrane transferred to lid of Petri dish and wrapped in foil for freezing and shipping, and (D) a frozen plate returned from low Earth orbit.

Ground control Veggie unit at Kennedy Space Center (left) and both Veggie units at capacity aboard the ISS (right).

Success criteria of EVT samples as measured across 60 plates_

Success Criteria	Goal	Observed	Score
Germination	>90%	99%	Excellent
radical emergence	>90%	99%	Excellent
Growth	90%	>99%	Excellent
roots > 4cm	90%	>99%	Excellent
Contamination	<10%	0%	Excellent
Bacterial or fungal growth	<10%	0%	Excellent
Seedling intactness	>90%	100%	Excellent
Dissectible into roots/shoots	>90%	100%	Excellent
RNA quality	>75%	100%	Excellent
RIN> 8, 260/280>2	>75%	100%	Excellent

References

Authors

Metrics

Articles in this issue

DOI: https://doi.org/10.2478/gsr-2022-0004 | Journal eISSN: 2332-7774

Journal RSS Feed

Language: English

Page range: 30 - 36

Published on: Dec 31, 2022

Published by: American Society for Gravitational and Space Research

In partnership with: Paradigm Publishing Services

Publication frequency: Volume open

Keywords:

Spaceflight,

advanced plant habitat,

microgravity

Related subjects:

Life sciences,

Life sciences, other,

Materials sciences,

Materials sciences, other,

Physics,

Physics, other

© 2022 Alexander Meyers, Eric Land, Imara Perera, Emma Canaday, Sarah E. Wyatt, published by American Society for Gravitational and Space Research
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 10 (2022): Issue 1 (January 2022)

Polyethersulfone (PES) Membrane on Agar Plates as a Plant Growth Platform for Spaceflight

Figures & Tables

Figure 1

Figure 2

Figure 3

Figure 4

Success criteria of EVT samples as measured across 60 plates_

Paradigm

My account