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Growth and Development of Ecotypes of Arabidopsis thaliana: Preliminary Experiments to Prepare for a Moon Lander Mission Cover

Growth and Development of Ecotypes of Arabidopsis thaliana: Preliminary Experiments to Prepare for a Moon Lander Mission

Gravitational and Space Research
Volume 8 (2020): Issue 1 (May 2020)
By: Tatsiana Shymanovich and  John Z. Kiss  
Open Access
|May 2020

Figures & Tables

Figure 1

Studies of seed germination of various ecotypes of Arabidopsis thaliana from different latitudes. Ecotypes are listed from north (63°N) to south (16°N) at the bottom of each plot. Seeds were incubated at the indicated temperatures in continuous illumination, and seed germination was assayed at days 3–10 after the start of the experiment. Note that the Col-0 ecotype had the highest germination under all conditions studied and nearly 100% in the temperature range of 11–30 °C. The sample sizes are provided in Table 2.
Studies of seed germination of various ecotypes of Arabidopsis thaliana from different latitudes. Ecotypes are listed from north (63°N) to south (16°N) at the bottom of each plot. Seeds were incubated at the indicated temperatures in continuous illumination, and seed germination was assayed at days 3–10 after the start of the experiment. Note that the Col-0 ecotype had the highest germination under all conditions studied and nearly 100% in the temperature range of 11–30 °C. The sample sizes are provided in Table 2.

Figure 2

Leaf development assayed by the (A) number of leaves and (B) total area of the leaves in the seedlings at the indicated temperatures in continuous illumination. In terms of the number of leaves (A), the Col-0 ecotype had the greatest number of leaves for all temperatures tested. As indicated by leaf area (B), Col-0 had the largest area when the data were taken together from 11 to 25 °C while the Mt ecotype was an overall second in this latter criterion. The sample sizes are provided in Table 2. Bars indicate SE.
Leaf development assayed by the (A) number of leaves and (B) total area of the leaves in the seedlings at the indicated temperatures in continuous illumination. In terms of the number of leaves (A), the Col-0 ecotype had the greatest number of leaves for all temperatures tested. As indicated by leaf area (B), Col-0 had the largest area when the data were taken together from 11 to 25 °C while the Mt ecotype was an overall second in this latter criterion. The sample sizes are provided in Table 2. Bars indicate SE.

Figure 3

Root development as determined by the (A) main root length, (B) number of secondary roots, and (C) length of the total root network of the seedlings at the indicated temperatures in continuous illumination. Based on these criteria, Col-0 exhibited the most robust growth from 11 to 25 °C. The sample sizes are provided in Table 2. Bars indicate SE.
Root development as determined by the (A) main root length, (B) number of secondary roots, and (C) length of the total root network of the seedlings at the indicated temperatures in continuous illumination. Based on these criteria, Col-0 exhibited the most robust growth from 11 to 25 °C. The sample sizes are provided in Table 2. Bars indicate SE.

Figure 4

Images of seedlings of the Col-0 ecotype of Arabidopsis thaliana after 10 days at 11, 15, 25, 30, and 35 °C. While there was good growth at 11 °C, the most robust plants were at incubation at 15–25 °C, and plants at these temperatures had a very extensive secondary root system. There was minimal growth at 30 °C and limited germination at 35 °C. Overall, this ecotype had the most robust growth at a wide temperature range. Note that in some images, there were reflections from the plastic Petri dishes. The large units on the scale reference are in centimeters.
Images of seedlings of the Col-0 ecotype of Arabidopsis thaliana after 10 days at 11, 15, 25, 30, and 35 °C. While there was good growth at 11 °C, the most robust plants were at incubation at 15–25 °C, and plants at these temperatures had a very extensive secondary root system. There was minimal growth at 30 °C and limited germination at 35 °C. Overall, this ecotype had the most robust growth at a wide temperature range. Note that in some images, there were reflections from the plastic Petri dishes. The large units on the scale reference are in centimeters.

Figure 5

Images of seedlings of the Mt ecotype of Arabidopsis thaliana after 10 days at 11, 15, 25, 30, and 30 °C. This ecotype was the second-best in overall growth characteristics and did particularly well at 25 °C. There was minimal growth at 30 °C and no germination or growth at 35 °C. Note that in some images, there were reflections from the plastic Petri dishes. The large units on the scale reference are in centimeters.
Images of seedlings of the Mt ecotype of Arabidopsis thaliana after 10 days at 11, 15, 25, 30, and 30 °C. This ecotype was the second-best in overall growth characteristics and did particularly well at 25 °C. There was minimal growth at 30 °C and no germination or growth at 35 °C. Note that in some images, there were reflections from the plastic Petri dishes. The large units on the scale reference are in centimeters.

Key characteristics of the seed stocks of the nine ecotypes used in these studies_

NameCodeLatitudeAltitude (m)Mean temperature (°C) Spring/FallOriginSourceStock #
TenelaTe-0N63°1–1000–2/7–8FinlandABRCCS1550
SwedenSWN62°NANASwedenMSU**
OsthammarOst-1N61°1–1000–2/5–6SwedenABRCCS1430
ColumbiaCol-0N52°1–10015–16/21–22PolandMSU**
DresdenDr-0N51°100–2007–8/9–10GermanyABRCCS1114
ItalyITN42°NANAItalyMSU**
CoimbraCo-2N40°100–20011–12/17–18PortugalABRCCS1086
MartubaMt-0N28°100–20015–16/15–16LibyaABRCCS22642
Cape Verde IsCvi-0N16°1200NACape VerdeABRCCS8580

Number of seedlings grown from each ecotype and used for the temperature experiments_

Seed codeGrowth temperature
11 °C15 °C25 °C30 °C35 °C
Te-0nt3624nt37
SW4836364836
Ost-1nt2424nt20
Col-04836364836
Dr-0nt3624nt36
IT4836334837
Co-2nt2724nt24
Mt-02336362536
Cvi-0nt3324nt36
DOI: https://doi.org/10.2478/gsr-2020-0002 | Journal eISSN: 2332-7774
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Language: English
Page range: 18 - 24
Published on: May 26, 2020
Published by: American Society for Gravitational and Space Research
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
Arabidopsis thaliana,
Artemis Program,
CubeSat,
Lunar Lander,
Plant Morphology,
Spaceflight
Related subjects:
Life sciences,
Life sciences, other,
Materials sciences,
Materials sciences, other,
Physics,
Physics, other

© 2020 Tatsiana Shymanovich, John Z. Kiss, published by American Society for Gravitational and Space Research
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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