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Impact of g-Load Shift on Temporal Expression Pattern of Apoptosis-linked Proteins in the Rat Mammary Gland Cover

Impact of g-Load Shift on Temporal Expression Pattern of Apoptosis-linked Proteins in the Rat Mammary Gland

Gravitational and Space Research
Volume 7 (2019): Issue 1 (August 2019)
By: Kibrom M. Alula,  James H. Resau and  Osman V. Patel  
Open Access
|Jun 2019

Figures & Tables

Figure 1

Representative images of lobular outlining (green) and unmixing for protein quantification. An overlay image (a) was unmixed into blue hematoxylin (b) and red VDR (c). The unmixed images of dual-labeled slide enable quantification of individual, as well as overlapping signals within the same outlined lobular border. The scale bar represents 50 mm (10× magnification) in the photomicrographs. VDR, vitamin D receptor.
Representative images of lobular outlining (green) and unmixing for protein quantification. An overlay image (a) was unmixed into blue hematoxylin (b) and red VDR (c). The unmixed images of dual-labeled slide enable quantification of individual, as well as overlapping signals within the same outlined lobular border. The scale bar represents 50 mm (10× magnification) in the photomicrographs. VDR, vitamin D receptor.

Figure 2

Quantitative comparison of mammary gland lobular CC-3 (a: within groups; b: between groups), P53 (c: within groups; d: between groups), and VDR (e: within groups; f: between groups) proteins measured in SC and HG-exposed rats following unmixing of chromophores using the CRi Nuance Multispectral Imaging System at G20, P1, and P3. Data are shown as mean ± SEM; *significantly different within and between each group (*p<0.05; **p<0.005; ***p<0.001; ****p<0.0001).G20, day 20 of gestation; HG, hypergravity; P1, postpartum day 1; P3, postpartum day 3; P53, tumor protein p53; SC, stationary control; SEM, standard error of the mean; VDR, vitamin D receptor.
Quantitative comparison of mammary gland lobular CC-3 (a: within groups; b: between groups), P53 (c: within groups; d: between groups), and VDR (e: within groups; f: between groups) proteins measured in SC and HG-exposed rats following unmixing of chromophores using the CRi Nuance Multispectral Imaging System at G20, P1, and P3. Data are shown as mean ± SEM; *significantly different within and between each group (*p<0.05; **p<0.005; ***p<0.001; ****p<0.0001).G20, day 20 of gestation; HG, hypergravity; P1, postpartum day 1; P3, postpartum day 3; P53, tumor protein p53; SC, stationary control; SEM, standard error of the mean; VDR, vitamin D receptor.
DOI: https://doi.org/10.2478/gsr-2019-0003 | Journal eISSN: 2332-7774
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Language: English
Page range: 13 - 21
Published on: Jun 17, 2019
Published by: American Society for Gravitational and Space Research
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
Rat,
Mammary Gland,
Gravity,
Apoptosis,
Lactation,
Pregnancy,
Immunohistochemistry
Related subjects:
Life sciences,
Life sciences, other,
Materials sciences,
Materials sciences, other,
Physics,
Physics, other

© 2019 Kibrom M. Alula, James H. Resau, Osman V. Patel, published by American Society for Gravitational and Space Research
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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