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Levels of Acid Sphingomyelinase (ASM) in Caenorhabditis elegans in Microgravity Cover

Levels of Acid Sphingomyelinase (ASM) in Caenorhabditis elegans in Microgravity

Open Access
|Jul 2020

Figures & Tables

Figure 1

Type 3 Fluids Mixing Enclosure (FME) Mini-Lab. The FME is 17 cm in length with a total volume of 8.4 mL divided into three 2.8 mL compartments. Volume 1 and 3 contained 2.4 mL of CeMM and Volume 2 contained 2.4 mL of CeMM housing 5,000 C. elegans.
Type 3 Fluids Mixing Enclosure (FME) Mini-Lab. The FME is 17 cm in length with a total volume of 8.4 mL divided into three 2.8 mL compartments. Volume 1 and 3 contained 2.4 mL of CeMM and Volume 2 contained 2.4 mL of CeMM housing 5,000 C. elegans.

Figure 2

Length and cross-sectional area (CSA) of ground and space worms. Representative images of (A) ground worms, and (B) space worms are shown. Live C. elegans appear curled and dead C. elegans assume a straight and thick shape. Only live C. elegans were measured. The space worms (N=40) were longer (C), and had larger CSA (D), compared to the control worms (N=40). Data are mean ± SD. ***p<0.05.
Length and cross-sectional area (CSA) of ground and space worms. Representative images of (A) ground worms, and (B) space worms are shown. Live C. elegans appear curled and dead C. elegans assume a straight and thick shape. Only live C. elegans were measured. The space worms (N=40) were longer (C), and had larger CSA (D), compared to the control worms (N=40). Data are mean ± SD. ***p<0.05.

Figure 3

Histograms of length and cross-sectional area (CSA) of space and control C. elegans. The top panels depict the distribution of lengths of randomly selected control C. elegans (n=40, left panel) and space C. elegans (n=40, right panel). Bottom panels similarly demonstrate the distribution of CSAs of control (left panel) and space (right panel) worms. A broader distribution of both length and CSA is evident in the space worms compared to the control ground worms.
Histograms of length and cross-sectional area (CSA) of space and control C. elegans. The top panels depict the distribution of lengths of randomly selected control C. elegans (n=40, left panel) and space C. elegans (n=40, right panel). Bottom panels similarly demonstrate the distribution of CSAs of control (left panel) and space (right panel) worms. A broader distribution of both length and CSA is evident in the space worms compared to the control ground worms.

Figure 4

Ground and space worm lysates immunoblotted for ASM-1 and ASM-2. The space worms exhibited decreased expression of both ASM-1 and ASM-2, compared to the ground worms (left panel ASM Western blot, right panel graph of quantified fluorescent signal). GAPDH immunostaining demonstrates equivalent protein loading. Data are mean ± SD. ***p<0.05, **p<0.1.
Ground and space worm lysates immunoblotted for ASM-1 and ASM-2. The space worms exhibited decreased expression of both ASM-1 and ASM-2, compared to the ground worms (left panel ASM Western blot, right panel graph of quantified fluorescent signal). GAPDH immunostaining demonstrates equivalent protein loading. Data are mean ± SD. ***p<0.05, **p<0.1.

Timeline of the experiment_ The letter A stands for arrival (at the ISS) and the letter U stands for undock from the ISS_

LocationTime StartTime DurationTemperature (°C)Changes made to the FME
Travel from Toronto to Houston (FedEx)A-25 days2 days2–4None
NanoRacks (Houston)A-23 days12 days2–4None
Travel from Ferry to LaunchA-11 days11 days2–4None
Rocket travel to ISSA-6 hours6 hours2–4None
ISSA6 weeks21–24A+0 days: Unclamp Clamp A, shake gently for 3 seconds U-14 days: Unclamp Clamp B, shake gently for 3 seconds
Travel from Landing to HoustonU4 days0–10None
Travel from Houston to Toronto (FedEx)U+4 days24 hours2–4None
Language: English
Page range: 27 - 36
Published on: Jul 20, 2020
Published by: American Society for Gravitational and Space Research
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year

© 2020 Annabel K. Gravely, Alice Vlasov, Amy Freeman, Kay Wu, Nathaniel J. Szewczyk, Robert D’Cruz, Jane Batt, published by American Society for Gravitational and Space Research
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.