Figure 1.
Overexpression of Hairless (H-C2) induces an ectopic vein phenotype in the adult Drosophila wing, but does not significantly affect stereotyped venation patterning_ Stereotyped and ectopic wing venation resulting from overexpression of H-C2 (Johannes and Preiss, 2002) was quantified by VESGEN in vascular maps (Figure 1) to obtain densities of vessel length (Lv, px px-2), vessel area (Av, px2 px-2), and vessel number (Nv, px-2)_ Results for the wild type and Class 5 wing are reproduced here_
| Phenotype | Wing Veins | Ectopic Veins | |||
|---|---|---|---|---|---|
| Lv | Av | Lv | Av | Nv | |
| Wild type | 0.0250 | 0.0789 | 0.0004 | 0.0006 | 1 |
| Class 5 veins Comparison to wild type | 0.0257 | 0.0892 | 0.0095 | 0.0254 | 18 |
Changes in mRNA expression of selected genes in space returned 3rd instar larvae that are involved in wing development_
| Gene Name | Fold Change | p-value | Biological Processes |
|---|---|---|---|
| vrille (CG14029) | -1.30 | 0.00 | Imaginal disc-derived wing hair organization and biogenesis |
| Absent, small, or homeotic discs 2 or ash2 (CG6677) | +0.60 | 0.00 | Imaginal disc-derived wing morphogenesis; Imaginal disc-derived wing vein specification; Phenotypes of alleles manifest in wing vein L3, wing margin |
| CTP:phospho-choline cytidylyl transferase 1 (CG1049) | +0.60 | 0.00 | Imaginal disc-derived wing morphogenesis |
| Pox neuro (CG8246) | -0.60 | 0.00 | Imaginal disc-derived wing morphogenesis; Phenotypes of alleles manifest in ventral wing blade |
| Bx42 (CG8264) | +0.50 | 0.00 | Notch signaling pathway; Phenotypes of alleles manifest in anterior cross vein |
Changes in mRNA expression of various genes of signaling pathways across wing disc and vein development in adult female flies_
| Gene Name | Fold Change | p-value | Biological Processes |
|---|---|---|---|
| Epidermal Growth Factor Receptor (EGFR) Signaling Pathway | |||
| stem cell tumor (CG33166) | -1.50 | 0.00 | EGFR signaling pathway; Wing vein morphogenesis; Phenotypes of alleles manifest in wing vein |
| aveugle (CG30476) | -0.80 | 0.00 | EGFR signaling pathway; Phenotypes of alleles manifest in wing vein and wing disc |
| rhomboid-4 (CG1697) | +0.70 | 0.00 | EGFR signaling pathway; Phenotypes of alleles manifest in wing and wing vein |
| rhomboid-7 (CG8972) | -0.70 | 0.00 | EGFR signaling pathway; Expressed in developing wing veins |
| pointed (CG17077) | +0.80 | 0.00 | EGFR signaling pathway; Imaginal disc-derived wing morphogenesis |
| Notch Signaling Pathway | |||
| Sp1070 (CG9138) | +2.20 | 0.00 | Negative regulation of Notch signaling pathway; Notch binding |
| bunched (CG5461) | +1.90 | 0.00 | Negative regulation of Notch signaling pathway; Phenotypes of alleles manifest in wing discs |
| brainiac (CG4934) | -0.90 | 0.00 | Notch signaling pathway |
| shibire (CG18102) | +2.10 | 0.00 | Positive regulation of Notch signaling pathway; Wing vein extension; Veined wing generated song production |
| Hedgehog Receptor Activity | |||
| Smoothened (CG11561) | -0.80 | 0.00 | Negative regulation of Notch signaling pathway; Wing disc anterior/posterior pattern formation; Smoothened signaling pathway |
| Other | |||
| discs overgrown (CG2048) | +0.80 | 0.00 | Establishment of imaginal disc-derived wing hair orientation |
| piopio (CG2079) | +1.50 | 0.00 | Apposition of dorsal and ventral imaginal disc-derived wing surfaces; Imaginal disc-derived wing morphogenesis |
| held out wings (CG10293) | +0.90 | 0.00 | Apposition of dorsal and ventral imaginal disc-derived wing surfaces |
| penguin (CG1685) | -0.80 | 0.00 | Apposition of dorsal and ventral imaginal disc-derived wing surfaces |
| guftagu (CG11861) | +0.70 | 0.00 | Imaginal disc-derived wing morphogenesis |
| Downstream of kinase (CG2079) | -1.10 | 0.00 | Imaginal disc-derived wing morphogenesis; Phenotypes of alleles manifest in wing |
| glut4EF (CG34360) | +1.30 | 0.00 | Imaginal disc-derived wing morphogenesis; Phenotypes of alleles manifest in wing |