Suborbital Vehicles to Study Transition Adaptation to Spaceflight – Why Biologists Should Care About the New Suborbital Flight Opportunities

Ferl, Robert J.

doi:10.2478/gsr-2014-0016

Abstract

The advent of the new generation of suborbital space vehicles is opening up a new and exciting realm of space science that should be of great interest to biologists. These vehicles make it possible to explore biological responses and adaptations that occur in the first few minutes of entering spaceflight and also in the first few minutes after return from space. Historically these transition stages in spaceflight have simply not been available for research, especially within human-rated vehicles. Given that complex biological responses are seldom linear over time, and that essentially all current experiments on the International Space Station (ISS) are conducted after stabilization on orbit, biologists are missing the chance to understand the pathways that lead from terrestrial existence to successful spaceflight adaptation and back. Studies conducted on suborbital spacecraft can therefore be an innovative approach to filling a substantial gap in knowledge regarding the temporal dynamics of biological responses to successful spaceflight physiological adaptation.

References

Aubry-Hivet D, Nziengui H, Rapp K, Oliveira O, Paponov IA, Li Y, Hauslage J, Vagt N, Braun M, Ditengou FA, Dovzhenko A, Palme K (2014) Analysis of gene expression during parabolic flights reveals distinct early gravity responses in Arabidopsis roots. Plant Biology 16 Suppl 1: 129–141
Search in Google Scholar Back to article
Correll MJ, Pyle TP, Millar KD, Sun Y, Yao J, Edelmann RE, Kiss JZ (2013) Transcriptome analyses of Arabidopsis thaliana seedlings grown in space: implications for gravity-responsive genes. Planta 238(3): 519–533
Search in Google Scholar Back to article
Decadal-Survey-Committee (2011) Recapturing a Future for Space Exploration: Life and Physical Sciences Research for a New Era. Washington, D.C.: The National Academies Press
Search in Google Scholar Back to article
Ferl RJ, Zupanska A, Spinale A, Reed D, Manning-Roach S, Guerra G, Cox DR, Paul A-L (2011) The performance of KSC Fixation Tubes with RNAlater for orbital experiments: A case study in ISS operations for molecular biology. Advances in Space Research 48(1): 199–206
Search in Google Scholar Back to article
Fowler S, Thomashow MF (2002) Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell 14(8): 1675–1690
Search in Google Scholar Back to article
Grosse J, Wehland M, Pietsch J, Ma X, Ulbrich C, Schulz H, Saar K, Hubner N, Hauslage J, Hemmersbach R, Braun M, van Loon J, Vagt N, Infanger M, Eilles C, Egli M, Richter P, Baltz T, Einspanier R, Sharbati S, Grimm D (2012) Short-term weightlessness produced by parabolic flight maneuvers altered gene expression patterns in human endothelial cells. FASEB Journal 26: 639–655
Search in Google Scholar Back to article
Hausmann N, Fengler S, Hennig A, Franz-Wachtel M, Hampp R, Neef M (2014) Cytosolic calcium, hydrogen peroxide and related gene expression and protein modulation in Arabidopsis thaliana cell cultures respond immediately to altered gravitation: parabolic flight data. Plant Biology 16 Suppl 1: 120–128
Search in Google Scholar Back to article
Kast EJ, Nguyen MD, Lawrence RE, Rabeler C, Kaplinsky NJ (2013) The RootScope: a simple high-throughput screening system for quantitating gene expression dynamics in plant roots. BMC Plant Biology 13: 158
Search in Google Scholar Back to article
Kimbrough JM, Salinas-Mondragon R, Boss WF, Brown CS, Sederoff HW (2004) The fast and transient transcriptional network of gravity and mechanical stimulation in the Arabidopsis root apex. Plant Physiology 136: 2790–2805
Search in Google Scholar Back to article
Kiss JZ, Millar KD, Edelmann RE (2012) Phototropism of Arabidopsis thaliana in microgravity and fractional gravity on the International Space Station. Planta 236(2): 635–645
Search in Google Scholar Back to article
Matia I, González-Camacho F, Herranz R, Kiss JZ, Gasset G, van Loon JJ, Marco R, Medina FJ (2010) Plant cell proliferation and growth are altered by microgravity conditions in spaceflight. Journal of Plant Physiology 167(3): 184–193
Search in Google Scholar Back to article
Nakashima J, Liao F, Sparks JA, Tang Y, Blancaflor EB (2014) The actin cytoskeleton is a suppressor of the endogenous skewing behaviour of Arabidopsis primary roots in microgravity. Plant Biology 16 Suppl 1: 142–150
Search in Google Scholar Back to article
Paul A-L, Amalfitano CE, Ferl RJ (2012a) Plant growth strategies are remodeled by spaceflight. BioMed Central Plant Biology 12: 232
Search in Google Scholar Back to article
Paul A-L, Daugherty CJ, Bihn EA, Chapman DK, Norwood KL, Ferl RJ (2001) Transgene expression patterns indicate that spaceflight affects stress signal perception and transduction in Arabidopsis. Plant Physiology 126(2): 613–621
Search in Google Scholar Back to article
Paul A-L, Manak MS, Mayfield JD, Reyes MF, Gurley WB, Ferl RJ (2011) Parabolic flight induces changes in gene expression patterns in Arabidopsis thaliana. Astrobiology 11(8): 743–758
Search in Google Scholar Back to article
Paul A-L, Popp MP, Gurley WB, Guy CL, Norwood KL, Ferl RJ (2005) Arabidopsis gene expression patterns are altered during spaceflight. Advances in Space Research 36(7): 1175–1181
Search in Google Scholar Back to article
Paul A-L, Wheeler RM, Levine HG, Ferl RJ (2013a) Fundamental plant biology enabled by the space shuttle. American Journal of Botany 100(1): 226–234
Search in Google Scholar Back to article
Paul A-L, Zupanska A, Ostrow DT, Zhang Y, Sun Y, Li JL, Shanker S, Farmerie WG, Amalfitano CE, Ferl RJ (2012b) Spaceflight transcriptomes: unique responses to a novel environment. Astrobiology 12(1): 40–56
Search in Google Scholar Back to article
Paul A-L, Zupanska AK, Schultz ER, Ferl RJ (2013b) Organ-specific remodeling of the Arabidopsis transcriptome in response to spaceflight. BioMed Central Plant Biology 13: 112
Search in Google Scholar Back to article
Ruyters G, Braun M (2014) Plant biology in space: recent accomplishments and recommendations for future research. Plant Biology 16 Suppl 1: 4–11
Search in Google Scholar Back to article
Salmi ML, Bushart TJ, Roux SJ (2011a) Autonomous gravity perception and responses of single plant cells. Gravitational and Space Biology 25(1): 6–13
Search in Google Scholar Back to article
Salmi ML, Roux SJ (2008) Gene expression changes induced by spaceflight in single-cells of the fern Ceratopteris richardii. Planta 229(1): 151–159
Search in Google Scholar Back to article
Salmi ML, ul Haque A, Bushart TJ, Stout SC, Roux SJ, Porterfield DM (2011b) Changes in gravity rapidly alter the magnitude and direction of a cellular calcium current. Planta 233(5): 911–920
Search in Google Scholar Back to article
Scherer GFE, Pietrzyk P (2014) Gravity-dependent differentiation and root coils in Arabidopsis thaliana wild type and phospholipase-A-I knockdown mutant grown on the International Space Station. Plant Biology 16 Suppl 1: 97–106
Search in Google Scholar Back to article
Stutte GW, Monje O, Hatfield RD, Paul A-L, Ferl RJ, Simone CG (2006) Microgravity effects on leaf morphology, cell structure, carbon metabolism, and mRNA expression of dwarf wheat. Planta 224: 1038–1049
Search in Google Scholar Back to article
Sugimoto M, Oono Y, Gusev O, Matsumoto T, Yazawa T, Levinskikh MA, Sychev VN, Bingham GE, Wheeler R, Hummerick M (2014) Genome-wide expression analysis of reactive oxygen species gene network in Mizuna plants grown in long-term spaceflight. BioMed Central Plant Biology 14: 4
Search in Google Scholar Back to article
Takahashi H (2011) Hydrotropism and auxininducible gene expression in roots grown under microgravity conditions. In NASA ISS Payload Fact Sheet. Retrieved from http://www.nasa.gov/mission_pages/station/research/experiments/766.html; (Accessed 11/25/14)
Search in Google Scholar Back to article
Wolverton C, Kiss JZ (2009) An update on plant space biology. Gravitational and Space Biology 22: 13–20
Search in Google Scholar Back to article
Wyatt SE, Kiss JZ (2013) Plant tropisms: From Darwin to the International Space Station. American Journal of Botany 100: 1–3
Search in Google Scholar Back to article

Suborbital Vehicles to Study Transition Adaptation to Spaceflight – Why Biologists Should Care About the New Suborbital Flight Opportunities

Abstract

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