The Genetic Analysis of Potential New HEV-3 Subtype Identified on Pig Farm in Slovakia

Božena Kočíková; Alica Pavlová; Katarína Dudášová; René Mandelík; Anna Jacková

doi:10.2478/fv-2023-0012

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The Genetic Analysis of Potential New HEV-3 Subtype Identified on Pig Farm in Slovakia

Folia Veterinaria

Volume 67 (2023): Issue 2 (June 2023)

By: Božena Kočíková, Alica Pavlová, Katarína Dudášová, René Mandelík and Anna Jacková

Open Access

|Jun 2023

Abstract

Hepatitis E virus (HEV) represents the causative agent of acute viral hepatitis in the world. The virus belongs to the genus Paslahepevirus, species Paslahepevirus balayani, which contains 8 different genotypes and at least 14 reference HEV-3 subtypes. Recently we have identified three isolates in a Slovakian pig farm as a new unclassified HEV-3 subtype. The aim of this study was to sequence and analyze additional regions of HEV genome for PER5 isolate, a representative isolate of potential new HEV-3 subtype. The phylogenetic analyses of 749 bp and 828 bp DNA fragments amplified from ORF1 revealed that both sequences fall outside two major clades (3abchij and 3efg) of the HEV-3 genotype as well as the other HEV-3 subtypes. The nucleotide identity with other HEV-3 subtypes indicated low values within the range 79.2–84.1 % as well. The results provide further support for the circulation of a new HEV-3 subtype in the pig farm in Slovakia.

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Articles in this issue

DOI: https://doi.org/10.2478/fv-2023-0012 | Journal eISSN: 2453-7837 | Journal ISSN: 0015-5748

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Language: English

Page range: 11 - 17

Submitted on: Feb 3, 2023

Accepted on: Mar 22, 2023

Published on: Jun 19, 2023

Published by: The University of Veterinary Medicine and Pharmacy in Košice

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

HEV-3,

Related subjects:

Microbiology and virology,

Medicine,

Veterinary medicine

© 2023 Božena Kočíková, Alica Pavlová, Katarína Dudášová, René Mandelík, Anna Jacková, published by The University of Veterinary Medicine and Pharmacy in Košice
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 67 (2023): Issue 2 (June 2023)