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Effect of heat acclimation on thermotolerance of in vitro strawberry plantlets Cover

Effect of heat acclimation on thermotolerance of in vitro strawberry plantlets

Open Access
|Apr 2024

Figures & Tables

Figure 1.

Overview of the control, heat acclimation and non-acclimation treatments used in this study.
Overview of the control, heat acclimation and non-acclimation treatments used in this study.

Figure 2.

Morphology of strawberry plantlets 21 days after lethal temperature exposure. (A) T0 plantlet with healthy green leaves. (B) T3 plantlet with deterioration of original leaves and emergence of new leaves. (C) TN plantlet with deterioration of original leaves without new leaf growth. Bar = 1 cm.
Morphology of strawberry plantlets 21 days after lethal temperature exposure. (A) T0 plantlet with healthy green leaves. (B) T3 plantlet with deterioration of original leaves and emergence of new leaves. (C) TN plantlet with deterioration of original leaves without new leaf growth. Bar = 1 cm.

Figure 3.

Activity of SOD (A), APX (B), GR (C), CAT (D) and POD (E) of strawberry plantlets following heat acclimation and lethal heat stress from 25°C to 48°C. Values are mean ± SE. Values followed by different letters are significantly different at p < 0.05, separated by Duncan’s multiple range test. APX, ascorbate peroxidase; CAT, catalase; GR, glutathione reductase; POD, peroxidase; SOD, superoxide dismutase.
Activity of SOD (A), APX (B), GR (C), CAT (D) and POD (E) of strawberry plantlets following heat acclimation and lethal heat stress from 25°C to 48°C. Values are mean ± SE. Values followed by different letters are significantly different at p < 0.05, separated by Duncan’s multiple range test. APX, ascorbate peroxidase; CAT, catalase; GR, glutathione reductase; POD, peroxidase; SOD, superoxide dismutase.

Figure 4.

Antioxidant enzyme activities of the strawberry plantlets following the control (T0), heat-acclimation treatment (T3) and non-acclimation treatment (TN). (A) SOD, (B) APX, (C) GR, (D) CAT and (E) POD. Values are means ± SE. Values followed by different letters are significantly different at p < 0.05, separated by Duncan’s multiple range test. APX, ascorbate peroxidase; CAT, catalase; GR, glutathione reductase; POD, peroxidase; SOD, superoxide dismutase.
Antioxidant enzyme activities of the strawberry plantlets following the control (T0), heat-acclimation treatment (T3) and non-acclimation treatment (TN). (A) SOD, (B) APX, (C) GR, (D) CAT and (E) POD. Values are means ± SE. Values followed by different letters are significantly different at p < 0.05, separated by Duncan’s multiple range test. APX, ascorbate peroxidase; CAT, catalase; GR, glutathione reductase; POD, peroxidase; SOD, superoxide dismutase.

Survival rates of heat-acclimated strawberry plantlets at different days after lethal heat stress_

Treatment7 days14 days21 days28 days
T0100 a100 a100 a100 a
T155 ± 0.31 b55 ± 0.10 b75 ± 0.36 b75 ± 0.36 b
T255 ± 0.10 b60 ± 0.00 b85 ± 0.36 b95 ± 0.32 ab
T370 ± 0.00 b85 ± 0.58 ab95 ± 0.14 ab100 a
T480 ± 0.26 b95 ± 0.32 a100 a100 a
TN0 c0 c0 c0 c

Number of new adventitious roots produced by heat-acclimated strawberry plantlets at different days after lethal heat stress_

Treatment7 Days14 Days21 Days28 Days35 Days42 Days49 Days56 Days
T01.40 ± 0.40 a2.70 ± 0.56 a4.10 ± 0.72 a5.00 ± 0.98 a5.70 ± 1.09 a6.40 ± 1.28 a6.50 ± 1.32 a6.80 ± 1.31 a
T1000.40 ± 0.31 bc1.10 ± 0.46 bc1.10 ± 0.46 cd1.80 ± 0.55 cd2.90 ± 0.80 b3.10 ± 0.77 b
T200.30 ± 0.21 bc0.60 ± 0.31 bc1.40 ± 0.69 bc1.50 ± 0.67 cd2.10 ± 0.64 c2.60 ± 0.82 b3.00 ± 0.84 b
T30.40 ± 0.27 b0.90 ± 0.28 b1.60 ± 0.37 b2.60 ± 0.50 b3.70 ± 0.50 b4.40 ± 0.50 b4.70 ± 0.50 ab4.80 ± 0.49 ab
T400.40 ± 0.22 bc1.30 ± 0.47 b1.80 ± 0.44 bc2.90 ± 0.43 bc3.20 ± 0.51 bc3.80 ± 0.61 b3.90 ± 0.60 b

Number of new leaves produced by heat-acclimated strawberry plantlets at different days after lethal heat stress_

Treatment7 Days14 Days21 Days28 Days35 Days42 Days49 Days56 Days
T00.80 ± 0.13 a1.30 ± 0.21 ab2.20 ± 0.20 b3.10 ± 0.18 c4.20 ± 0.20 b5.10 ± 0.23 b5.70 ± 0.15 a6.00 ± 0.21 a
T10.10 ± 0.10 c1.00 ± 0.33 b2.10 ± 0.43 b3.50 ± 0.45 c4.40 ± 0.56 b5.00 ± 0.65 b5.80 ± 0.74 a6.30 ± 0.83 a
T201.30 ± 0.42 ab3.50 ± 0.43 a4.80 ± 0.42 a6.00 ± 0.54 a6.50 ± 0.45 a6.60 ± 0.43 a6.60 ± 0.43 a
T30.40 ± 0.16 b2.10 ± 0.31 a3.50 ± 0.40 a4.60 ± 0.54 ab5.20 ± 0.51 ab5.70 ± 0.45 ab6.50 ± 0.40 a7.40 ± 0.45 a
T40.40 ± 0.16 b1.50 ± 0.22 ab2.50 ± 0.22 b3.70 ± 0.30 bc4.60 ± 0.27 b5.40 ± 0.27 ab6.00 ± 0.26 a6.70 ± 0.33 a

Number of new lateral roots produced by heat-acclimated strawberry plantlets at different days after lethal heat stress_

Treatment7 Days14 Days21 Days28 Days35 Days42 Days49 Days56 Days
T000.80 ± 0.51bc1.20 ± 0.76 cd1.50 ± 0.82 c1.50 ± 0.82 c1.50 ± 0.82 cd1.50 ± 0.82 cd1.50 ± 0.82 c
Tl00.60 ± 0.50 bc1.80 ± 0.94 bcd2.40 ± 1.09 bc2.40 ± 1.09 bc2.90 ± 1.15 bc3.50 ± 1.22 bc4.60 ± 1.53 b
T20.50 ± 0.50 a1.60 ± 1.08 abc3.20 ± 1.44 abc4.50 ± 1.27 ab4.70 ± 1.21 ab5.10 ± 1.22 ab5.60 ± 1.14 ab6.10 ± 1.22 ab
T302.20 ± 0.66 ab4.80 ± 0.76 a5.60 ± 0.81 a5.70 ± 0.80 a6.30 ± 0.78 a7.00 ± 0.76 a7.20 ± 0.79 ab
T40.20 ± 0.20 a3.00 ± 0.67 a4.20 ± 0.79 ab4.50 ± 0.89 ab5.10 ± 0.81 a6.50 ± 0.64 a7.40 ± 0.52 a7.90 ± 0.38 a

Chlorophylls, electrolyte leakage and free proline content of heat-acclimated strawberry plantlets after lethal heat stress_

TreatmentChlorophyll (mg · g-1 FW)Electrolyte leakage (%)Proline (mg · g-1 FW)
TotalCh aCh b
T03.92 ± 0.02 a2.81 ± 0.02 a1.11 ± 0.04 a10.76 ± 0.04 c1.38 ± 0.41 b
T31.81 ± 0.04 b1.26 ± 0.02 b0.54 ± 0.03 b22.46 ± 0.70 b3.34 ± 0.13 a
TN1.11 ± 0.01 c0.75 ± 0.01 c0.36 ± 0.02 c38.26 ± 0.62 a1.69 ± 0.09 b
DOI: https://doi.org/10.2478/fhort-2024-0008 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761
Language: English
Page range: 135 - 147
Submitted on: Aug 7, 2023
Accepted on: Feb 28, 2024
Published on: Apr 24, 2024
Published by: Polish Society for Horticultural Sciences (PSHS)
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year

© 2024 Zevon Julio Seymour, Juan Francisco Mercedes, Jong-Yi Fang, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.