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Evaluation of alternative substrates for hydroponics based on biological parameters of leaf lettuce (Lactuca sativa L.) and its stress response Cover

Evaluation of alternative substrates for hydroponics based on biological parameters of leaf lettuce (Lactuca sativa L.) and its stress response

Open Access
|Jun 2023

Figures & Tables

Table 1.

Physical and chemical parameters of substrates.

SubstrateBulk densityTotal pore spaceAir capacityTotal water-holding capacityChlorides in drainECpH
Unitkg · L–1%%L · m–3g · L–1mS · cm–1
Mineral wool0.06497.1315.68150.0060.2476.6
Perlite  0.1159.97       303000.008  0.08287.99
Coco peat0.08177.48  4.47300.0290.5284.85
Wood fibre0.064          7612.86320.0110.2486.25
Sheep wool0.04896.77       771980.094    6.247.97

1 The pH values and electrical conductivity were measured in soil:distilled water (1:2.5 = v:v) suspension by using an electrometric method. Mohr’s method was used to determine the chloride ion concentration of a solution by titration with silver nitrate.

Table 2.

Elements content in the drains from the substrates (mg · L–1).

ElementPerliteMineral woolWood fibreSheep woolCoco peat
P0.4 ± 0.0 a0.2 ± 0.0 a2.6 ± 0.0 b22.5 ± 0.5 d4.3 ± 0.1 c
K30.4 ± 1.1 c12.1 ± 0.2 a22.6 ± 0.7 b2,995.9 ± 1.5 e103.0 ± 3.0 d
S0.9 ± 0.1 a45.3 ± 1.8 c1.6 ± 0.0 a69.3 ± 1.4 d10.8 ± 0.4 b
Ca4.6 ± 0.5 a14.6 ± 0.5 b7.0 ± 0.5 a126.6 ± 1.2 d32.6 ± 1.4 c
Na4.8 ± 0.3 a43.9 ± 1.9 c6.1 ± 0.2 a57.5 ± 2.0 d33.7 ± 0.8 b
Fe1.7 ± 0.5 b0.3 ± 0.1 a0.2 ± 0.0 a10.9 ± 0.2 d4.2 ± 0.1 c
Mg1.1 ± 0.2 a4.8 ± 0.0 a2.5 ± 0.1 a30.1 ± 2.6 c12.5 ± 0.2 b
Zn0.0 ± 0.0 a0.0 ± 0.0 a0.1 ± 0.0 a1.2 ± 0.1 c0.2 ± 0.0 b
Sum44.0 ± 0.8 a121.2 ± 3.5 b42.8 ± 1.8 a3,314.0 ± 7.8 d201.4 ± 5.4 c

1 The table shows the average values with standard deviation.

1 Different letters for individual values indicate significant differences at p ≤ 0.05.

Figure 1.

Leaf biomass (g). Different letters above the individual columns indicate significant differences at p ≤ 0.05. Error bars represent standard deviation.

Figure 2.

Number of leaves (pcs). Different letters above individual columns indicate significant differences at p ≤ 0.05. Error bars represent standard deviation.

Figure 3.

Stem diameter (mm). Different letters above individual columns indicate significant differences at p ≤ 0.05. Error bars represent standard deviation.

Figure 4.

Leaf area of the entire head of lettuce (cm2). Different letters above individual columns indicate significant differences at p ≤ 0.05. Error bars represent standard deviation.

Table 3.

Analytical parameters of lettuce. The table shows average values with standard deviation.

Substrate PerliteAA (% scavenging of DPPH)GSH (mg · g–1 DM)APX (AsA · g–1 FM · min–1)TPC (mg GAE · g–1 FM)Nitrates (mg · kg–1 FM)
Perlite21.6 ± 4.9 a44.0 ± 19.7 a108.8 ± 58.5 a43.3 ± 0.9 a604.2 ± 110.3 ab
Rockwool32.6 ± 3.0 ab22.5 ± 12.1 a129.6 ± 25.6 a40.6 ± 1.6 a555.1 ± 58.1 ab
Wood fibre46.1 ± 5.9 bc31.0 ± 4.9 a105.0 ± 32.2 a55.4 ± 7.9 b447.6 ± 38.2 a
Sheep wool54.9 ± 11.5 c23.1 ± 5.2 a135.9 ± 24.5 a74.0 ± 1.3 c686.2 ± 86.5 b
Coco peat40.3 ± 2.7 abc19.9 ± 2.5 a126.4 ± 25.5 a48.0 ± 1.6 ab552.6 ± 69.9 ab

1 Different letters for individual values indicate significant differences at p ≥ 0.05.

1 AA, antioxidant activity; APX, ascorbate peroxidase; AsA, ascorbic acid; DM, dry mass; DPPH, 2,2-diphenyl-1-picrylhydrazyl; FM, fresh mass; GAE, gallic acid equivalents; GSH, glutathione; TPC, total phenol content expressed as GAE.

Figure 5.

Graph of multiple regression analysis between AA (% scavenging of DPPH) and content of TPC (p = 0.002449). AA, antioxidant activity; DPPH, 2,2-diphenyl-1-picrylhydrazyl; FM, fresh mass; TPC, total phenol content.

DOI: https://doi.org/10.2478/fhort-2023-0006 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761
Language: English
Page range: 77 - 90
Submitted on: Dec 14, 2022
Accepted on: Feb 2, 2023
Published on: Jun 26, 2023
Published by: Polish Society for Horticultural Sciences (PSHS)
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Related subjects:

© 2023 Vojtech Ferby, Tomas Kopta, Monika Komorowska, Maciej Fidurski, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.