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Screening of composite substrates suitable for tissue-cultured plantlets growth of Asparagus cochinchinensis (Lour.) Merr Cover

Screening of composite substrates suitable for tissue-cultured plantlets growth of Asparagus cochinchinensis (Lour.) Merr

Folia Horticulturae
Volume 34 (2022): Issue 1 (June 2022)
By: ,  ,  ,  ,   and    
Open Access
|Mar 2022
Figures & tables

Figures & Tables

Table 1

Combination ratio of different substrates.

CodeVolume ratio (%)
Garden soilPerliteCoconut branVermiculitePeat
T1100.000000
T220.0040.0040.0000
T320.00040.0040.000
T420.000040.0040.00
T520.0040.000040.00
T6033.3333.3333.330
T7033.33033.3333.33

[i] T1: garden soil 100%; T2: garden soil:perlite:coconut bran = 20%: 40%:40%; T3: garden soil:coconut bran:vermiculite = 20%:40%:40%; T4: garden soil:vermiculite:peat = 20%:40%:40%; T5: garden soil: perlite:peat = 20%:40%:40%; T6: perlite:coconut bran:vermiculite = 33.33%:33.33%:33.33%; T7: perlite:vermiculite:peat = 33.33%: 33.33%:33.33%.

Table 2

The physical and chemical properties of different substrates combinations.

CodeBulk density (g · cm−3)Total porosity (%)Aeration porosity (%)Water retaining porosity (%)Void ratioEC (mS · cm−1)pH
Means ± SD
T10.98 ± 0.03 a42.67 ± 0.57 e7.44 ± 0.10 e35.25 ± 0.67 d0.21 ± 0.01 e1.60 ± 0.02 a5.79 ± 0.01 e
T20.38 ± 0.00 cd55.58 ± 1.20 d13.16 ± 0.64 d42.42 ± 0.98 bc0.31 ± 0.01 cd0.78 ± 0.01 b5.93 ± 0.08 d
T30.46 ± 0.01 b59.19 ± 2.09 bc11.10 ± 0.46 d48.09 ± 2.55 a0.23 ± 0.02 de0.67 ± 0.02 c5.98 ± 0.04 cd
T40.43 ± 0.03 bc59.31 ± 2.73 bc16.12 ± 0.87 c43.18 ± 3.60 b0.38 ± 0.05 c0.60 ± 0.03 d6.08 ± 0.04 b
T50.36 ± 0.00 d57.60 ± 0.47 c19.39 ± 1.45 b38.21 ± 1.91 cd0.51 ± 0.06 ab0.51 ± 0.02 e6.06 ± 0.03 bc
T60.23 ± 0.05 e65.07 ± 0.61 a21.30 ± 1.25 ab43.77 ± 0.65 ab0.49 ± 0.04 b0.51 ± 0.01 e6.30 ± 0.01 a
T70.24 ± 0.03 e62.01 ± 1.90 ab22.72 ± 0.86 a39.29 ± 1.03 bcd0.58 ± 0.01 a0.49 ± 0.01 f6.32 ± 0.02 a

[i] Different lowercase letters in the same column indicate significant differences between treatments (p < 0.05).

[ii] EC, electrical conductivity.

Figure 1

The effect of different combinations of substrates on the survival rate of A. cochinchinensis plantlets. Different lowercase letters indicate significant differences between treatments (p < 0.05). Error bars represent SD and data in the graphs are the means ± SD. T1: garden soil 100%; T2: garden soil:perlite:coconut bran = 20%:40%:40%; T3: garden soil:coconut bran:vermiculite = 20%:40%:40%; T4: garden soil:vermiculite:peat = 20%:40%:40%; T5: garden soil:perlite:peat = 20%:40%:40%; T6: perlite:coconut bran:vermiculite = 33.33%:33.33%:33.33%; T7: perlite:vermiculite:peat = 33.33%:33.33%:33.33%. SD, standard deviation.

Figure 2

Effects of different combined substrates on above-ground growth of A. cochinchinensis. Error bars represent SD and data in the graphs are the means ± standard deviation. T1: garden soil 100%; T2: garden soil: perlite: coconut bran = 20%: 40%: 40%; T3: garden soil: coconut bran: vermiculite = 20%: 40%: 40%; T4: garden soil: vermiculite: peat = 20%: 40%: 40%; T5: garden soil: perlite: peat = 20%: 40%: 40%; T6: perlite: coconut bran: vermiculite = 33.33%: 33.33%: 33.33%; T7: perlite: vermiculite: peat = 33.33%: 33.33%: 33.33%.

Figure 3

Root morphology of A. cochinchinensis plantlet in different substrates. T1: garden soil 100%; T2: garden soil:perlite:coconut bran = 20%:40%:40%; T3: garden soil:coconut bran:vermiculite = 20%:40%:40%; T4: garden soil:vermiculite:peat = 20%:40%:40%; T5: garden soil:perlite:peat = 20%:40%:40%; T6: perlite:coconut bran:vermiculite = 33.33%:33.33%:33.33%; T7: perlite:vermiculite:peat = 33.33%:33.33%:33.33%.

Figure 4

Effects of different combined substrates on underground parameters of A. cochinchinensis. Error bars represent SD and data in the graphs are the means ± SD. T1: garden soil 100%; T2: garden soil:perlite:coconut bran = 20%:40%:40%; T3: garden soil:coconut bran:vermiculite = 20%:40%:40%; T4: garden soil:vermiculite:peat = 20%:40%:40%; T5: garden soil:perlite:peat = 20%:40%:40%; T6: perlite:coconut bran:vermiculite = 33.33%:33.33%:33.33%; T7: perlite:vermiculite:peat = 33.33%:33.33%:33.33%. SD, standard deviation.

Figure 5

Effects of different combined substrates on physiology and biochemistry indexes of A. cochinchinensis. Different lowercase letters indicate significant differences between treatments (p = 0.05). Error bars represent SD and data in the graphs are the means ± SD. T1: garden soil 100%; T2: garden soil:perlite:coconut bran = 20%:40%:40%; T3: garden soil:coconut bran:vermiculite = 20%:40%:40%; T4: garden soil:vermiculite:peat = 20%:40%:40%; T5: garden soil:perlite:peat = 20%:40%:40%; T6: perlite:coconut bran:vermiculite = 33.33%:33.33%:33.33%; T7: perlite:vermiculite:peat = 33.33%:33.33%:33.33%. SD, standard deviation.

Table 3

Correlation analysis between physical properties of substrate and growth parameters and physiology and biochemistry indexes of tissue-cultured seedlings of A. cochinchinensis.

IndexBulk densityTotal porosityAeration porosityWater retaining porosityVoid ratioConductivity valuepH value
Survival rate−0.987**0.912**0.771*0.5170.668−0.952**0.729
Aboveground
Plant height−0.853*0.7300.6310.3950.538−0.7540.579
Stem diameter−0.4640.6000.2440.6920.086−0.3930.486
Fresh weight−0.7010.6080.5930.2390.505−0.5550.524
Dry weight−0.7160.6170.6290.2070.547−0.5630.559
Underground
Root fresh weight−0.6580.6530.5370.3900.410−0.5250.557
Root dry weight−0.773*0.6980.6480.3190.544−0.6130.631
Root length−0.5550.5970.5530.2740.440−0.4070.629
Root surface area−0.5530.5690.4480.3660.323−0.4180.486
Root diameter−0.5980.5960.3080.5990.163−0.5510.287
Root volume−0.5080.4820.3610.3340.247−0.3640.359
Physiology
Soluble protein−0.7490.6960.3150.758*0.175−0.6980.375
Soluble sugar−0.6920.6480.827*−0.0040.795*−0.6370.715
MDA0.681−0.543−0.544−0.193−0.4700.505−0.465
Total chlorophyll−0.4490.3730.1710.4040.057−0.3500.129
Proline−0.760*0.6480.5660.3420.466−0.6090.509
Root vigour−0.5680.6100.2520.6970.097−0.5800.248

* Means significant (p < 0.05).

** Means extremely significant (p < 0.01).

MDA, malondialdehyde.

Table 4

Membership function values and comprehensive ranking.

Evaluation indexMembership function values of each evaluation index of seven substrates
T1T2T3T4T5T6T7
Survival rate0.000.950.780.760.900.981.00
Aboveground
Plant height0.001.000.420.740.550.770.79
Stem diameter0.000.291.000.000.001.000.57
Fresh weight0.000.760.170.270.541.000.36
Dry weight0.000.750.150.270.531.000.41
Underground
Root fresh weight0.000.560.230.260.351.000.25
Root dry weight0.000.750.250.290.461.000.51
Total root length0.000.260.080.080.121.000.19
Root surface area0.000.460.200.130.2710.13
Root diameter0.000.830.470.360.521.000.23
Root volume0.000.610.170.100.321.000.05
Physiology
Soluble protein0.001.000.980.650.710.600.87
Soluble sugar0.000.290.110.290.821.000.47
MDA0.000.9640.150.230.5410.46
Total chlorophyll0.001.000.170.700.280.750.15
Proline0.000.940.320.360.561.000.50
Root vigour0.000.871.000.670.880.970.13
Average value0.000.720.390.360.490.950.42
Ranking7256314

[i] T1: garden soil 100%; T2: garden soil:perlite:coconut bran = 20%:40%:40%; T3: garden soil:coconut bran:vermiculite = 20%:40%:40%; T4: garden soil:vermiculite: peat = 20%:40%:40%; T5: garden soil:perlite:peat = 20%:40%:40%; T6: perlite:coconut bran:vermiculite = 33.33%:33.33%:33.33%; T7: perlite:vermiculite:peat = 33.33%:33.33%:33.33%.

[ii] MDA, malondialdehyde.

Figure 6

Growth status of A. cochinchinensis plantlet in different substrates. T1: garden soil 100%; T2: garden soil:perlite:coconut bran = 20%:40%:40%; T3: garden soil:coconut bran:vermiculite = 20%:40%:40%; T4: garden soil:vermiculite:peat = 20%:40%:40%; T5: garden soil:perlite:peat = 20%:40%:40%; T6: perlite:coconut bran:vermiculite = 33.33%:33.33%:33.33%; T7: perlite:vermiculite:peat = 33.33%:33.33%:33.33%.

DOI: https://doi.org/10.2478/fhort-2022-0004 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761
Journal RSS Feed
Language: English
Page range: 39 - 50
Submitted on: Oct 17, 2021
Accepted on: Feb 2, 2022
Published on: Mar 7, 2022
Published by: Polish Society for Horticultural Sciences (PSHS)
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
aboveground growth,
combined substrate,
physiology and biochemistry,
underground growth
Related subjects:
Life sciences,
Plant science

© 2022 Xiaosong Yu, Lin Li, Yongquan Li, Zhi Liu, Ningxian Yang, Mingsheng Zhang, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 34 (2022): Issue 1 (June 2022)
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