Figure 1
![Concentration of (A) macroelements and (B) microelements in substrates [mg · 100 g−1 substrate] (one-way ANOVA with a post-hoc Tukey's test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. Fresh Substrate Control; ^p < 0.05, ^^p < 0.01, ^^^p < 0.001 vs. Substrate from Flush I Control; #p < 0.05 vs. Substrate from Flush II Control).](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6471d0ff215d2f6c89db1c50/j_fhort-2022-0002_fig_001.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=ASIA6AP2G7AKEKTCLMLW%2F20260307%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20260307T201810Z&X-Amz-Expires=3600&X-Amz-Security-Token=IQoJb3JpZ2luX2VjEDoaDGV1LWNlbnRyYWwtMSJIMEYCIQC%2Fxhs1Kd9l5r4RaUPFU%2FzUlkTv9yqe7BRpa2hfBT40UgIhANB2SYCiKOP93f23zYQbJ%2BywNP40nmePfWZYUTXuvrigKr0FCAMQAhoMOTYzMTM0Mjg5OTQwIgzBigjGLAOtxe8Uw48qmgVvdTIqjNxQYQovwLJn2CXhGwzgX67SoO8PBbBvvQqBo%2BogEJwGeHj1aJR5HcRECK%2FV1Y8L%2FEe3IYLLfSzt6%2Bmmcwhhk3w%2FMBe9dIYFIZUhwdkFqkeC0PujB5Y1cV3yUzNdsvl%2B7D5H3AwVR78q2EcR2jxW7eLBatme%2Fo9tBWe38hhY7CAIDLLYHIWEHTVSDT2tN1X4EiOwYRivmWZhs8OfGdqZqmOEUveN5dN3ZP5g4PZFh0GWyvXoucKE%2BTnNO%2BrLsbPyOIAd8DzwBAK2vMvV1P%2BGti1WPdQDhC94c3dOfC%2BZv9LYfH%2F02DkQV4Sqh%2F6nKAxcqJ7V9QwPFm1iNToPG%2FdGJ8WQvSr17criLCfSYiDnOt%2BEkDMRPLPtalC85fCH94xxRAP2fO1FE%2B3ZToVRJI6PGujsGyGfutE0rH90toaxih%2FvmZG5mPl1kPQ6QGuI8oQAv5iHgC97w2lhVcSW%2BLBV6NgUcJGjgQaIWbaeL%2FaIdoQnOBrgP1xL2JZzZ7BmhFWaCgUIj1%2FupdzhG2oGdlcdMUJMRyDy3MWz9RlkOt3AZJeN5uOZgNvH5oRzNVSmqiVtFEcjNvsLstJT%2Bsx8jRjs99WVeV6XAYyO4Lyt3nMkkRwCUKboiMixLCdE0iBo34lFd5O2dEIl24BE%2FGD0kja8jgVG1g6aoAWosBDFPr%2BeO9%2BPh31GBhb1Fe4x9VjQ9dUHQ1DrhP6gfeGSyCY7YtdkMEEaHMvgBwzfhFioJT%2Bm764wL0VZQX5Ep6JulI531N2zfJPjh4F3H5SbChdYW8LCe8Zn0Vb5G8Z6Jgqz6PaIExm%2BknlXGhXiZ9eLjQfrHbi08EIQsvgWFmAdrR6vH%2BkfcOzx3lmMMLvw5VBSbiIH3FfiM3k80B0w%2Fc%2BxzQY6sAFDPIoJg2OXJ%2BM58bA4kxSTzIYRSUPKYbqYPO7MR5YHaX1jl3mqqQLLz%2FLTVcCYgRH3seYD6O4L2sqHgZ%2FFj%2Fc%2FsT2nPQgKbOodPPU%2FiDQ5RISVlsxtuIMmwBT4PXwymctN1E%2FJYu1x0vTZVCmbI8K1zwF108nlOQbBNpwLv1JVuMpKo%2B6ahl2Mq9Lw9kKE0RFcvLq%2BWLVPioSvjKsqtIXM7zYK95H4acoJtoVSX1YiHA%3D%3D&X-Amz-Signature=67fca5432a1e6739f03e8be6049eeaf85d6d302cd78bc97ce975ad562237e9ac&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject)
Figure 2
![Concentration of (A) macroelements and (B) microelements in fruiting bodies of A. bisporus obtained from three cultivation flushes [mg · 100 g−1 dry weight] (one-way ANOVA with a post-hoc Tukey's test: *p < 0.05, **p < 0.01 vs. A. bisporus Flush I Control; ^p < 0.05, ^^p < 0.01 vs. A. bisporus Flush II Control).](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6471d0ff215d2f6c89db1c50/j_fhort-2022-0002_fig_002.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=ASIA6AP2G7AKEKTCLMLW%2F20260307%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20260307T201810Z&X-Amz-Expires=3600&X-Amz-Security-Token=IQoJb3JpZ2luX2VjEDoaDGV1LWNlbnRyYWwtMSJIMEYCIQC%2Fxhs1Kd9l5r4RaUPFU%2FzUlkTv9yqe7BRpa2hfBT40UgIhANB2SYCiKOP93f23zYQbJ%2BywNP40nmePfWZYUTXuvrigKr0FCAMQAhoMOTYzMTM0Mjg5OTQwIgzBigjGLAOtxe8Uw48qmgVvdTIqjNxQYQovwLJn2CXhGwzgX67SoO8PBbBvvQqBo%2BogEJwGeHj1aJR5HcRECK%2FV1Y8L%2FEe3IYLLfSzt6%2Bmmcwhhk3w%2FMBe9dIYFIZUhwdkFqkeC0PujB5Y1cV3yUzNdsvl%2B7D5H3AwVR78q2EcR2jxW7eLBatme%2Fo9tBWe38hhY7CAIDLLYHIWEHTVSDT2tN1X4EiOwYRivmWZhs8OfGdqZqmOEUveN5dN3ZP5g4PZFh0GWyvXoucKE%2BTnNO%2BrLsbPyOIAd8DzwBAK2vMvV1P%2BGti1WPdQDhC94c3dOfC%2BZv9LYfH%2F02DkQV4Sqh%2F6nKAxcqJ7V9QwPFm1iNToPG%2FdGJ8WQvSr17criLCfSYiDnOt%2BEkDMRPLPtalC85fCH94xxRAP2fO1FE%2B3ZToVRJI6PGujsGyGfutE0rH90toaxih%2FvmZG5mPl1kPQ6QGuI8oQAv5iHgC97w2lhVcSW%2BLBV6NgUcJGjgQaIWbaeL%2FaIdoQnOBrgP1xL2JZzZ7BmhFWaCgUIj1%2FupdzhG2oGdlcdMUJMRyDy3MWz9RlkOt3AZJeN5uOZgNvH5oRzNVSmqiVtFEcjNvsLstJT%2Bsx8jRjs99WVeV6XAYyO4Lyt3nMkkRwCUKboiMixLCdE0iBo34lFd5O2dEIl24BE%2FGD0kja8jgVG1g6aoAWosBDFPr%2BeO9%2BPh31GBhb1Fe4x9VjQ9dUHQ1DrhP6gfeGSyCY7YtdkMEEaHMvgBwzfhFioJT%2Bm764wL0VZQX5Ep6JulI531N2zfJPjh4F3H5SbChdYW8LCe8Zn0Vb5G8Z6Jgqz6PaIExm%2BknlXGhXiZ9eLjQfrHbi08EIQsvgWFmAdrR6vH%2BkfcOzx3lmMMLvw5VBSbiIH3FfiM3k80B0w%2Fc%2BxzQY6sAFDPIoJg2OXJ%2BM58bA4kxSTzIYRSUPKYbqYPO7MR5YHaX1jl3mqqQLLz%2FLTVcCYgRH3seYD6O4L2sqHgZ%2FFj%2Fc%2FsT2nPQgKbOodPPU%2FiDQ5RISVlsxtuIMmwBT4PXwymctN1E%2FJYu1x0vTZVCmbI8K1zwF108nlOQbBNpwLv1JVuMpKo%2B6ahl2Mq9Lw9kKE0RFcvLq%2BWLVPioSvjKsqtIXM7zYK95H4acoJtoVSX1YiHA%3D%3D&X-Amz-Signature=e4b8430e5c723cdad6f162aefd3e2e9a1b933ca2fe2672ffb520009c31eb0527&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject)
Figure 3
Content of organic compounds and total phenols and antioxidant activity (DPPH) of A_ bisporus extracts from three flushes_
| Compounds/flush | I | II | III |
|---|---|---|---|
| Phenolic compounds (mg · 100 g−1 d.w.) | |||
| Gallic acid | 18.20 ± 0.40 | 12.91 ± 0.20**** | 15.0 ± 0.40**** ^^ |
| p-Hydroxybenzoic acid | 2.26 ± 0.002 | 1.37 ± 0.003**** | 1.84 ± 0.002**** ^^^^ |
| Rutoside | 0.07 ± 0.003 | 0.12 ± 0.004**** | 0.01 ± 0.001**** ^^^^ |
| Quercetin | 7.17 ± 0.02 | 5.11 ± 0.002**** | 5.18 ± 0.05**** |
| Total phenols | 47.43 ± 1.52 | 43.81 ± 0.93** | 45.51 ± 3.7*** ^^ |
| Indole compounds (mg · 100 g−1 d.w.) | |||
| L-Tryptophan | 221.71 ± 0.9 | 118.30 ± 0.2**** | 170.82 ± 1.0**** ^^^^ |
| 5-HTP | 213.99 ± 5.71 | 145.64 ± 5.45**** | 199.56 ± 3.45* ^^^^ |
| Serotonin | 63.08 ± 1.00 | 36.42 ± 1.77**** | 70.08 ± 4.19* ^^^^ |
| Sterols (mg · 100 g−1 d.w.) | |||
| Ergosterol | 64.32 ± 0.08 | 30.53 ± 0.89**** | 3.56 ± 0.51**** ^^^^ |
| Ergosterol peroxide | 23.08 ± 0.34 | 17.71 ± 0.04*** | 13.71 ± 0.41*** ^^^ |
| Ergocalciferol | – | – | – |
| Other compounds (mg · 100 g−1 d.w.) | |||
| Phenylalanine | 386.2 ± 14.80 | 254.1 ± 9.41**** | 300.2 ± 11.71*** ^^ |
| Lovastatin | 5.71 ± 0.35 | 1.69 ± 0.13**** | 3.47 ± 0.32*** ^^^ |
| Ergothioneine | 10.88 ± 0.98 | 7.56 ± 0.83 | 14.74 ± 3.92^ |
| DPPH (%) | 89.41 ± 0.90 | 82.32 ± 3.20 | 76.71 ± 1.40 |