Figure 1
Concentration of (A) macroelements and (B) microelements in substrates [mg · 100 g−1 substrate] (one-way ANOVA with a post-hoc Tukey's test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. Fresh Substrate Control; ^p < 0.05, ^^p < 0.01, ^^^p < 0.001 vs. Substrate from Flush I Control; #p < 0.05 vs. Substrate from Flush II Control).
Figure 2
Concentration of (A) macroelements and (B) microelements in fruiting bodies of A. bisporus obtained from three cultivation flushes [mg · 100 g−1 dry weight] (one-way ANOVA with a post-hoc Tukey's test: *p < 0.05, **p < 0.01 vs. A. bisporus Flush I Control; ^p < 0.05, ^^p < 0.01 vs. A. bisporus Flush II Control).
Figure 3
The value of the BCF coefficient for individual macro- and microelements calculated in relation to the fresh substrate (column chart) and to the substrates obtained after the set of projections (line charts). BCF, bioconcentration factor.
Table 1
Content of organic compounds and total phenols and antioxidant activity (DPPH) of A. bisporus extracts from three flushes.
| Compounds/flush | I | II | III |
|---|---|---|---|
| Phenolic compounds (mg · 100 g−1 d.w.) | |||
| Gallic acid | 18.20 ± 0.40 | 12.91 ± 0.20**** | 15.0 ± 0.40**** ^^ |
| p-Hydroxybenzoic acid | 2.26 ± 0.002 | 1.37 ± 0.003**** | 1.84 ± 0.002**** ^^^^ |
| Rutoside | 0.07 ± 0.003 | 0.12 ± 0.004**** | 0.01 ± 0.001**** ^^^^ |
| Quercetin | 7.17 ± 0.02 | 5.11 ± 0.002**** | 5.18 ± 0.05**** |
| Total phenols | 47.43 ± 1.52 | 43.81 ± 0.93** | 45.51 ± 3.7*** ^^ |
| Indole compounds (mg · 100 g−1 d.w.) | |||
| L-Tryptophan | 221.71 ± 0.9 | 118.30 ± 0.2**** | 170.82 ± 1.0**** ^^^^ |
| 5-HTP | 213.99 ± 5.71 | 145.64 ± 5.45**** | 199.56 ± 3.45* ^^^^ |
| Serotonin | 63.08 ± 1.00 | 36.42 ± 1.77**** | 70.08 ± 4.19* ^^^^ |
| Sterols (mg · 100 g−1 d.w.) | |||
| Ergosterol | 64.32 ± 0.08 | 30.53 ± 0.89**** | 3.56 ± 0.51**** ^^^^ |
| Ergosterol peroxide | 23.08 ± 0.34 | 17.71 ± 0.04*** | 13.71 ± 0.41*** ^^^ |
| Ergocalciferol | – | – | – |
| Other compounds (mg · 100 g−1 d.w.) | |||
| Phenylalanine | 386.2 ± 14.80 | 254.1 ± 9.41**** | 300.2 ± 11.71*** ^^ |
| Lovastatin | 5.71 ± 0.35 | 1.69 ± 0.13**** | 3.47 ± 0.32*** ^^^ |
| Ergothioneine | 10.88 ± 0.98 | 7.56 ± 0.83 | 14.74 ± 3.92^ |
| DPPH (%) | 89.41 ± 0.90 | 82.32 ± 3.20 | 76.71 ± 1.40 |