Figure 1
![Concentration of (A) macroelements and (B) microelements in substrates [mg · 100 g−1 substrate] (one-way ANOVA with a post-hoc Tukey's test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. Fresh Substrate Control; ^p < 0.05, ^^p < 0.01, ^^^p < 0.001 vs. Substrate from Flush I Control; #p < 0.05 vs. Substrate from Flush II Control).](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6471d0ff215d2f6c89db1c50/j_fhort-2022-0002_fig_001.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=ASIA6AP2G7AKDYYDAVY4%2F20260307%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20260307T183929Z&X-Amz-Expires=3600&X-Amz-Security-Token=IQoJb3JpZ2luX2VjEDUaDGV1LWNlbnRyYWwtMSJHMEUCIDgcv8MakFdSSmq7krOm1%2BhmtUlrbbyuBWsYB4T4CqkuAiEA8RslYzveE%2BvBtMK5ZH7AZH7HV6rnXBXNEGWUGAqXZoYqxgUI%2Fv%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FARACGgw5NjMxMzQyODk5NDAiDBG2rFKxsNXTGsyLnCqaBRbHZSU8OVbQ4Mb4gGuA66LWZh7RPKs4qEVsoDUyKbGZiFgTRrIB8tNuwehh8rDueDWD%2B0ANcILb%2F9oWik6M3A7k%2FmPnx%2BpFkQ0ELJW7zquuORhr7n75rSZBSYHi7hUh5PbXpzl%2Fp8VqHOv8DrXPN0gmAt1qyCXgw5vl0W6NYw6HXp1sjtktSt8apsQW14K9AJDR7GKh%2BbCXXh4z612MQZEKtg8GqPICnDEL1j8uCFj6dWwiTDUWYiZlV8xIYLB0AysLDUoEa%2B1XftwRXdqjKV0nrSX19kZbClyKdyYW2%2Fwyp3x6A9ed3eUTmYEdeMigd8rOVhWNOvnyMmias5bf%2FqyxDe%2FJT4HIn2HG7OzvkoPcULatJZz%2F2e9subBRk0%2FBHm7EjvrorsE1bYeSVgxnSORt4%2BcWNul7uMjxqrg6Dftha9wBo6gtSjaSM6PTu1g1IF32C59JiTYvPfcPRoaW7DUCpa8V0wNc2VIh2sdJvtaz9t9QtAuSjSednQIJbKUg377%2FHEr09l7%2FHS7qiV3WrTQgCCRVSLcS%2FqCtUkadf03pSvu9c8FiMCGWFM7%2FVO12xNLyXgiMKq%2Fg%2F91qOXZJnRdQ6hpLzHKAuYYssLefOVThwQ4%2FJzf0Bp1TaZC8nnH%2B%2F6w4L8miq6gdu9TiJYdhCb3FeZ0osvaC0NsH5AiVf7z5U3fvG8C18Y0GAzwYL3y2XnEOyfebEa%2FMAHgOY9gAGK0N5oro%2B59FodnycJiGH%2FY74boc8xXR4ABQuagXS1b1a7We3pIr5t9heU4eqt0yH%2F3C5JkYP5tidZsqcAuZ0nHF2q%2Bjknn38lynvPqJmQrNJtFw8LI6o%2BQ%2B3Q1LSgGGFZV90Uaj01%2FIr9F20hiZvNFhTzDu6bsSMBKOjDCjvbDNBjqxAdM5YWoeS0rXrsml0TcRJn33jXoaWUN1%2BQFcvtgkx7xvNZz2npgWBTUbEkE4bTlfvltalonIWfriJ7ZeuRvHVGXMpZi%2BvwMS27fRotRRUiFPuBD3EXVFT%2BjhZZYHRLTEijl9eukT22gYJU%2FSJRYcZ4ztOmhdQGg6C%2FbIfN9ytewLVf5%2B8%2FpWeBOEK9qvnSatUGXKpyZ9TMHco8yTnBPlMS5hcCOPtgEKf%2F8wxgeY%2Fv5Nsw%3D%3D&X-Amz-Signature=377af904985c9f658cd702860f733b7a85e59220e581a0658c52c53339130359&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject)
Figure 2
![Concentration of (A) macroelements and (B) microelements in fruiting bodies of A. bisporus obtained from three cultivation flushes [mg · 100 g−1 dry weight] (one-way ANOVA with a post-hoc Tukey's test: *p < 0.05, **p < 0.01 vs. A. bisporus Flush I Control; ^p < 0.05, ^^p < 0.01 vs. A. bisporus Flush II Control).](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6471d0ff215d2f6c89db1c50/j_fhort-2022-0002_fig_002.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=ASIA6AP2G7AKDYYDAVY4%2F20260307%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20260307T183929Z&X-Amz-Expires=3600&X-Amz-Security-Token=IQoJb3JpZ2luX2VjEDUaDGV1LWNlbnRyYWwtMSJHMEUCIDgcv8MakFdSSmq7krOm1%2BhmtUlrbbyuBWsYB4T4CqkuAiEA8RslYzveE%2BvBtMK5ZH7AZH7HV6rnXBXNEGWUGAqXZoYqxgUI%2Fv%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FARACGgw5NjMxMzQyODk5NDAiDBG2rFKxsNXTGsyLnCqaBRbHZSU8OVbQ4Mb4gGuA66LWZh7RPKs4qEVsoDUyKbGZiFgTRrIB8tNuwehh8rDueDWD%2B0ANcILb%2F9oWik6M3A7k%2FmPnx%2BpFkQ0ELJW7zquuORhr7n75rSZBSYHi7hUh5PbXpzl%2Fp8VqHOv8DrXPN0gmAt1qyCXgw5vl0W6NYw6HXp1sjtktSt8apsQW14K9AJDR7GKh%2BbCXXh4z612MQZEKtg8GqPICnDEL1j8uCFj6dWwiTDUWYiZlV8xIYLB0AysLDUoEa%2B1XftwRXdqjKV0nrSX19kZbClyKdyYW2%2Fwyp3x6A9ed3eUTmYEdeMigd8rOVhWNOvnyMmias5bf%2FqyxDe%2FJT4HIn2HG7OzvkoPcULatJZz%2F2e9subBRk0%2FBHm7EjvrorsE1bYeSVgxnSORt4%2BcWNul7uMjxqrg6Dftha9wBo6gtSjaSM6PTu1g1IF32C59JiTYvPfcPRoaW7DUCpa8V0wNc2VIh2sdJvtaz9t9QtAuSjSednQIJbKUg377%2FHEr09l7%2FHS7qiV3WrTQgCCRVSLcS%2FqCtUkadf03pSvu9c8FiMCGWFM7%2FVO12xNLyXgiMKq%2Fg%2F91qOXZJnRdQ6hpLzHKAuYYssLefOVThwQ4%2FJzf0Bp1TaZC8nnH%2B%2F6w4L8miq6gdu9TiJYdhCb3FeZ0osvaC0NsH5AiVf7z5U3fvG8C18Y0GAzwYL3y2XnEOyfebEa%2FMAHgOY9gAGK0N5oro%2B59FodnycJiGH%2FY74boc8xXR4ABQuagXS1b1a7We3pIr5t9heU4eqt0yH%2F3C5JkYP5tidZsqcAuZ0nHF2q%2Bjknn38lynvPqJmQrNJtFw8LI6o%2BQ%2B3Q1LSgGGFZV90Uaj01%2FIr9F20hiZvNFhTzDu6bsSMBKOjDCjvbDNBjqxAdM5YWoeS0rXrsml0TcRJn33jXoaWUN1%2BQFcvtgkx7xvNZz2npgWBTUbEkE4bTlfvltalonIWfriJ7ZeuRvHVGXMpZi%2BvwMS27fRotRRUiFPuBD3EXVFT%2BjhZZYHRLTEijl9eukT22gYJU%2FSJRYcZ4ztOmhdQGg6C%2FbIfN9ytewLVf5%2B8%2FpWeBOEK9qvnSatUGXKpyZ9TMHco8yTnBPlMS5hcCOPtgEKf%2F8wxgeY%2Fv5Nsw%3D%3D&X-Amz-Signature=21750f4c61a118ecebbb4199b2705dad3c4c9e452a92bf4fc13b9c1fa648d5fd&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject)
Figure 3
Content of organic compounds and total phenols and antioxidant activity (DPPH) of A_ bisporus extracts from three flushes_
| Compounds/flush | I | II | III |
|---|---|---|---|
| Phenolic compounds (mg · 100 g−1 d.w.) | |||
| Gallic acid | 18.20 ± 0.40 | 12.91 ± 0.20**** | 15.0 ± 0.40**** ^^ |
| p-Hydroxybenzoic acid | 2.26 ± 0.002 | 1.37 ± 0.003**** | 1.84 ± 0.002**** ^^^^ |
| Rutoside | 0.07 ± 0.003 | 0.12 ± 0.004**** | 0.01 ± 0.001**** ^^^^ |
| Quercetin | 7.17 ± 0.02 | 5.11 ± 0.002**** | 5.18 ± 0.05**** |
| Total phenols | 47.43 ± 1.52 | 43.81 ± 0.93** | 45.51 ± 3.7*** ^^ |
| Indole compounds (mg · 100 g−1 d.w.) | |||
| L-Tryptophan | 221.71 ± 0.9 | 118.30 ± 0.2**** | 170.82 ± 1.0**** ^^^^ |
| 5-HTP | 213.99 ± 5.71 | 145.64 ± 5.45**** | 199.56 ± 3.45* ^^^^ |
| Serotonin | 63.08 ± 1.00 | 36.42 ± 1.77**** | 70.08 ± 4.19* ^^^^ |
| Sterols (mg · 100 g−1 d.w.) | |||
| Ergosterol | 64.32 ± 0.08 | 30.53 ± 0.89**** | 3.56 ± 0.51**** ^^^^ |
| Ergosterol peroxide | 23.08 ± 0.34 | 17.71 ± 0.04*** | 13.71 ± 0.41*** ^^^ |
| Ergocalciferol | – | – | – |
| Other compounds (mg · 100 g−1 d.w.) | |||
| Phenylalanine | 386.2 ± 14.80 | 254.1 ± 9.41**** | 300.2 ± 11.71*** ^^ |
| Lovastatin | 5.71 ± 0.35 | 1.69 ± 0.13**** | 3.47 ± 0.32*** ^^^ |
| Ergothioneine | 10.88 ± 0.98 | 7.56 ± 0.83 | 14.74 ± 3.92^ |
| DPPH (%) | 89.41 ± 0.90 | 82.32 ± 3.20 | 76.71 ± 1.40 |