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Evaluation of the possibility of obtaining viable seeds from the cross-breeding Hippeastrum × chmielii Chm. with selected cultivars of Hippeastrum hybridum Hort. Cover

Evaluation of the possibility of obtaining viable seeds from the cross-breeding Hippeastrum × chmielii Chm. with selected cultivars of Hippeastrum hybridum Hort.

Folia Horticulturae
Volume 33 (2021): Issue 1 (June 2021)
By: ,   and    
Open Access
|Jun 2021
Figures & tables

Figures & Tables

Figure 1

Clones of H. × chmielii – No. 6 (left) and No. 18 (right).

Figure 2

Cultivars of H. hybridum tested – ‘Gervase’, ‘Rio Negro’ and ‘Royal Velvet’ (from left to right).

Figure 3

The receptive stigma of Hippeastrum × chmielii stained by alizarine red – clone No. 6 (A) and clone No. 18 (B).

Figure 4

The receptive ovule of Hippeastrum × chmielii stained by alizarine red – clone No. 6 (A) and clone No. 18 (B).

Table 1

Percentage of viable/stainable pollen grains of cultivars H. hybridum – ‘Gervase’; ‘Royal Velvet’; ‘Rio Negro’.

CultivarsTotal number of pollen grain ± SDNumber of viable pollen grains ± SDPercentage of viable pollen grains (%) ± SD
H. hybridum ‘Gervase’195 ± 4.08162 ± 0.8283.0 ± 0.06 b*
H. hybridum ‘Royal Velvet’177 ± 1.63145 ± 3.3082.7 ± 5.51 b
H. hybridum ‘Rio Negro’285 ± 4.08210 ± 1.6366.4 ± 9.21 a

* Means ± standard deviation in a column followed by the same letter does not differ significantly at p = 0.05.

Figure 5

Pollen grains stained by acetocarmine – H. hybridum ‘Gervase’ (A), H. hybridum ‘Rio Negro’ (B) and H. hybridum ‘Royal Velvet’ (C). White arrows show viable pollen grains; black arrows show non-stainable/non-viable grains.

Figure 6

Sprouting pollen tubes on the germination medium – H. hybridum ‘Rio Negro’ (A) and H. hybridum ‘Royal Velvet’ (B).

Figure 7

Germinating pollen grains of H. hybridum ‘Rio Negro’ on the stigma (A) and pollen tubes of ‘Rio Negro’ penetrated the ovule (B).

Table 2

The number of seeds obtained from individual crossings of H. × chmielii with three cultivars.

CrossingNumber of flowersNumber of collected seeds from one flower (min – max)Average number of collected seeds per flower ± SDTotal number of seeds
H. × chmielii 18 × ‘Gervase’1031–7657.7 ± 15.53577
H. × chmielii 18 × ‘Royal Velvet’1524–8659.1 ± 17.99828
H. × chmielii 18 × ‘Rio Negro’1722–6843.6 ± 17.68305
H. × chmielii 6 × ‘Gervase’1025–9448.6 ± 22.17340
H. × chmielii 6 × ‘Royal Velvet’1553–8370.9 ± 9.44993
H. × chmielii 6 × ‘Rio Negro’10000
Total number of seeds from all crossings3,043
Table 3

Percentage of germinated seeds of H. × chmielii pollinated by pollen of three cultivars depending on the germination method.

CrossingGermination on Petri dishes (%)*Germination in water in the jars (%)*
H. × chmielii 18 × ‘Gervase’55.0 ± 5.61 a**71.2 ± 6.77 b
H. × chmielii 18 × ‘Royal Velvet’67.2 ± 9.54 a70.6 ± 8.66 a
H. × chmielii 18 × ‘Rio Negro’48.3 ± 6.01 a58.4 ± 5.80 a
H. × chmielii 6 × ‘Gervase’72.7 ± 12.02 a73.8 ± 10.88 a
H. × chmielii 6 × ‘Royal Velvet’75.4 ± 6.28 a77.9 ± 4.62 a

* Data after Bliss transformation.

** Means ± standard deviation in a row followed by the same letter does not differ significantly at p = 0.05.

DOI: https://doi.org/10.2478/fhort-2021-0014 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761
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Language: English
Page range: 185 - 194
Submitted on: Nov 24, 2020
Accepted on: Apr 19, 2021
Published on: Jun 5, 2021
Published by: Polish Society for Horticultural Sciences (PSHS)
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
amaryllis,
fertilisation,
germination,
pollen,
pollen tube,
stigma
Related subjects:
Life sciences,
Plant science

© 2021 Przemysław Marciniak, Agata Jędrzejuk, Dariusz Sochacki, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 33 (2021): Issue 1 (June 2021)
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