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Evaluation of the possibility of obtaining viable seeds from the cross-breeding Hippeastrum × chmielii Chm. with selected cultivars of Hippeastrum hybridum Hort. Cover

Evaluation of the possibility of obtaining viable seeds from the cross-breeding Hippeastrum × chmielii Chm. with selected cultivars of Hippeastrum hybridum Hort.

Folia Horticulturae
Volume 33 (2021): Issue 1 (June 2021)
By: Przemysław Marciniak,  Agata Jędrzejuk and  Dariusz Sochacki  
Open Access
|Jun 2021

Figures & Tables

Figure 1

Clones of H. × chmielii – No. 6 (left) and No. 18 (right).
Clones of H. × chmielii – No. 6 (left) and No. 18 (right).

Figure 2

Cultivars of H. hybridum tested – ‘Gervase’, ‘Rio Negro’ and ‘Royal Velvet’ (from left to right).
Cultivars of H. hybridum tested – ‘Gervase’, ‘Rio Negro’ and ‘Royal Velvet’ (from left to right).

Figure 3

The receptive stigma of Hippeastrum × chmielii stained by alizarine red – clone No. 6 (A) and clone No. 18 (B).
The receptive stigma of Hippeastrum × chmielii stained by alizarine red – clone No. 6 (A) and clone No. 18 (B).

Figure 4

The receptive ovule of Hippeastrum × chmielii stained by alizarine red – clone No. 6 (A) and clone No. 18 (B).
The receptive ovule of Hippeastrum × chmielii stained by alizarine red – clone No. 6 (A) and clone No. 18 (B).

Figure 5

Pollen grains stained by acetocarmine – H. hybridum ‘Gervase’ (A), H. hybridum ‘Rio Negro’ (B) and H. hybridum ‘Royal Velvet’ (C). White arrows show viable pollen grains; black arrows show non-stainable/non-viable grains.
Pollen grains stained by acetocarmine – H. hybridum ‘Gervase’ (A), H. hybridum ‘Rio Negro’ (B) and H. hybridum ‘Royal Velvet’ (C). White arrows show viable pollen grains; black arrows show non-stainable/non-viable grains.

Figure 6

Sprouting pollen tubes on the germination medium – H. hybridum ‘Rio Negro’ (A) and H. hybridum ‘Royal Velvet’ (B).
Sprouting pollen tubes on the germination medium – H. hybridum ‘Rio Negro’ (A) and H. hybridum ‘Royal Velvet’ (B).

Figure 7

Germinating pollen grains of H. hybridum ‘Rio Negro’ on the stigma (A) and pollen tubes of ‘Rio Negro’ penetrated the ovule (B).
Germinating pollen grains of H. hybridum ‘Rio Negro’ on the stigma (A) and pollen tubes of ‘Rio Negro’ penetrated the ovule (B).

The number of seeds obtained from individual crossings of H_ × chmielii with three cultivars_

CrossingNumber of flowersNumber of collected seeds from one flower (min – max)Average number of collected seeds per flower ± SDTotal number of seeds
H. × chmielii 18 × ‘Gervase’1031–7657.7 ± 15.53577
H. × chmielii 18 × ‘Royal Velvet’1524–8659.1 ± 17.99828
H. × chmielii 18 × ‘Rio Negro’1722–6843.6 ± 17.68305
H. × chmielii 6 × ‘Gervase’1025–9448.6 ± 22.17340
H. × chmielii 6 × ‘Royal Velvet’1553–8370.9 ± 9.44993
H. × chmielii 6 × ‘Rio Negro’10000
Total number of seeds from all crossings 3,043

Percentage of viable/stainable pollen grains of cultivars H_ hybridum – ‘Gervase’; ‘Royal Velvet’; ‘Rio Negro’_

CultivarsTotal number of pollen grain ± SDNumber of viable pollen grains ± SDPercentage of viable pollen grains (%) ± SD
H. hybridum ‘Gervase’195 ± 4.08162 ± 0.8283.0 ± 0.06 b*
H. hybridum ‘Royal Velvet’177 ± 1.63145 ± 3.3082.7 ± 5.51 b
H. hybridum ‘Rio Negro’285 ± 4.08210 ± 1.6366.4 ± 9.21 a

Percentage of germinated seeds of H_ × chmielii pollinated by pollen of three cultivars depending on the germination method_

CrossingGermination on Petri dishes (%)*Germination in water in the jars (%)*
H. × chmielii 18 × ‘Gervase’55.0 ± 5.61 a**71.2 ± 6.77 b
H. × chmielii 18 × ‘Royal Velvet’67.2 ± 9.54 a70.6 ± 8.66 a
H. × chmielii 18 × ‘Rio Negro’48.3 ± 6.01 a58.4 ± 5.80 a
H. × chmielii 6 × ‘Gervase’72.7 ± 12.02 a73.8 ± 10.88 a
H. × chmielii 6 × ‘Royal Velvet’75.4 ± 6.28 a77.9 ± 4.62 a
DOI: https://doi.org/10.2478/fhort-2021-0014 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761
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Language: English
Page range: 185 - 194
Submitted on: Nov 24, 2020
Accepted on: Apr 19, 2021
Published on: Jun 5, 2021
Published by: Polish Society for Horticultural Sciences (PSHS)
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
amaryllis,
fertilisation,
germination,
pollen,
pollen tube,
stigma
Related subjects:
Life sciences,
Plant science,
Zoology,
Ecology,
Life sciences, other

© 2021 Przemysław Marciniak, Agata Jędrzejuk, Dariusz Sochacki, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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