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Biomineralogical investigation of late-harvest grapes colonised by Botrytis cinerea Pers. Cover

Biomineralogical investigation of late-harvest grapes colonised by Botrytis cinerea Pers.

Open Access
|Oct 2020

Figures & Tables

Table 1

Basic aggregate parameters and contents of selected macronutrients in ‘Sibera’ and ‘Regent’ grape varieties

Stage of fruit maturationSoluble solids content (°Brix)Titratable acidity (g · 100 mL−1)pHK (mg · L−1)P (mg · L−1)Ca (mg · L−1)Mg (mg · L−1)
‘Sibera’
Physiological ripeness**18.1 a*1.02 b3.0 a865 a215 a91 a85 b
Late harvest21.4 b0.85 a3.4 b938 b237 b88 a72 a
‘Regent’
Physiological ripeness20.4 a0.87 b3.5 a1512 a191 a121 a93 a
Late harvest24.3 b0.75 a3.7 b1610 b223 b119 a89 a

* Means followed by the same letter within columns among cultivars do not differ significantly at p ≤ 0.05 by Duncan test.

** Harvest dates: ‘Sibera’ – physiological ripeness (3 October), late harvest (24 October); ‘Regent’ – physiological ripeness (30 September), late harvest (20 October).

Figure 1

‘Sibera’ variety. (A) Dried berry covered with a red growth. (B) Drying berry with sticky pink juice concentrated in morphological concavities. (C) Clusters of mycelial hyphae on the surface of the drying bunch at a skin rupture. (D) Abundant sporulation of B. cinerea on the surface a drying berry. Photomicrographs were taken with a binocular magnifier.

Figure 2

(A) Cracks in the skin of a drying berry; +1, +2 – EDS analysis locations. (B) mycelial hyphae on the edge of a skin crack. (C) - A cluster of mycelial hyphae and a cross section of a single hypha. (D) Conidia of B. cinerea. Scanning microscope, scale magnification.

Figure 3

EDS energy spectra of the skin surface of drying berries of the ‘Sibera’ variety at spots +1 (A) and +2 (B) shown in Figures 2A and 3C. EDS energy spectra obtained from the surface of the ‘Sibera’ variety grapes. Dried and cracked berries – red spectrum. Undamaged berries, less dry – blue spectrum.

Figure 4

‘Regent’ variety. (A) The surface of a drying berry covered with bright crystals of a potassium compound. (B) Abundant growth of B. cinerea air mycelium at a rupture of the peel of a ‘Regent’ berry. (C) Stalk with advancing mineralisation by red organic iron compounds. (D) Mineralisation by sugars at the location of a detached peduncle; visible glossy sugar microcrystals. Photomicrographs taken with a binocular magnifier.

Figure 5

Surface of ripe ‘Regent’ berries. (A) B. cinerea mycelium at the location of sugar microcrystals and potassium compounds. +1, +2 – EDS analysis locations. (B) Mycelial hyphae located within skin cracks. (C) A crystal of an organic compound containing potassium, located in the wound cavity caused by berry skin cracking.

Figure 6

EDS energy spectra obtained from the surface of the ‘Regent’ variety grapes. at points +1 (A), +2 (B) shown in Figure 5A and the crystal (C) shown in Figure 5C.

DOI: https://doi.org/10.2478/fhort-2020-0016 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761
Language: English
Page range: 171 - 178
Submitted on: Mar 10, 2020
Accepted on: Aug 14, 2020
Published on: Oct 17, 2020
Published by: Polish Society for Horticultural Sciences (PSHS)
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
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© 2020 Marek Grabowski, Maciej Pawlikowski, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.