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Mathematical models for the estimation of leaf chlorophyll content based on RGB colours of contact imaging with smartphones: A pomegranate example Cover

Mathematical models for the estimation of leaf chlorophyll content based on RGB colours of contact imaging with smartphones: A pomegranate example

Open Access
|Apr 2020

Figures & Tables

Figure 1

Experimental methodology and image acquisition: (A) selection of the leaf samples, (B): determination of the mid-point of the leaves, (C) a view of the red LED light from the closed box, (D) insertion of the leaf onto the closed box’s hole, (E) capturing of the contact image with a smartphone and (F) reading the RGB values of the images by using the histogram function of the Photoshop ME 7.0.

Figure 2

Captured leaf images with a smartphone when red LED passed (three red images from the left side) and when white LED passed (three green images from the right side).

Table 1

Correlation between the RGB colours and combination indices and leaf chlorophyll contents (for both mg ∙ g−1 and mg ∙ MW−1) of a pomegranate tree

RGB colours and combination indicesRed LED passedWhite LED passed
Chl (mg ∙ g−1)Chl (mg ∙ MW−1)Chl (mg ∙ g−1)Chl (mg ∙ MW−1)
R value−0.282−0.552**−0.225−0.257
G value0.1130.914**0.2100.460**
B value0.1460.893**−0.382*−0.364*
NRI−0.154−0.914**−0.195−0.424**
NGI0.1480.918**0.344*0.476**
NBI0.1720.897**−0.384*−0.371*
GMR0.2800.571**0.2790.484**
GMB0.0000.756**0.2870.456**
GRRI0.1460.917**0.2330.450**
GRVI0.1440.916**0.3020.475**
DGCI0.2800.574**−0.0140.009

* Correlation is significant at 0.05 level and

** at the 0.01 level (2-tailed).

Figure 3

Stepwise linear regression analysis for leaf Chl content measured analytically in laboratory and the RGB values based on contact imaging with smartphone.

Table 2

Summary results for multiple regression analysis among G value, B value and Chl (Y)

CoefficientsStandard errort Statp-value
Intercept−0.09140.017741−5.15181.18E-05
G0.15370.0553222.77820.008948
B−0.00190.071268−0.02710.978541
DOI: https://doi.org/10.2478/fhort-2020-0006 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761
Language: English
Page range: 57 - 67
Submitted on: Jan 15, 2020
Accepted on: Feb 13, 2020
Published on: Apr 22, 2020
Published by: Polish Society for Horticultural Sciences (PSHS)
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Related subjects:

© 2020 Nurdan Özreçberoğlu, İbrahim Kahramanoğlu, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.