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An efficient protocol for Cistus crispus L. (Cistaceae) micropropagation Cover

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An efficient protocol for Cistus crispus L. (Cistaceae) micropropagation

Folia Horticulturae

Volume 32 (2020): Issue 1 (June 2020)

By: Sergio Saia and Antonio Giovino

Open Access

|Jul 2020

Figures & tables

Figures & Tables

(A) Experimental unit at the beginning of the experiment: apical sprout from primary and secondary branches. (B) Experimental unit at the time of establishment in vitro.

Percentage of sterile (left panel) and vital explants (right panel) of Cistus crispus at increasing sodium hypochlorite concentration (CHC) and time of sterilisation (TS). Data are values of mean ± standard error. For sterile explants, CHC: F = 31.2, p < 0.001; TS: F = 423.6, p < 0.001; and CHC × TS: F = 2.5, p = 0.088. For vital explants, CHC: F = 135.2, p < 0.001; TS: F = 276.7, p < 0.001; and CHC × TS: F = 4.0, p = 0.022. When CHC × TS was significant, treatments were separated by t-grouping of the LSMEANS estimate. Treatments with a letter in common are not different at t0.05-grouping.

Coefficient of proliferation (c.p.), number of root (n.r.), and percentage of healthy explants (h.e.) of Cistus crispus cuttings at increasing benzylaminopurine (BA) concentration. Data are values of mean ± standard error. c.p.: F = 652.3, p < 0.001; n.r.: F = 15.1, p < 0.001; and h.e.: F = 314.3, p < 0.001. Within each variable, treatments with a letter in common are not different at p > 0.05 according to the Tukey's test applied to the LSMEANS estimates’ differences.

Coefficient of axillary shoot proliferation of Cistus crispus microcuttings at increasing concentration (CCYT) of cytokinins (CYTs): benzylaminopurine (BA), kinetin (Kin), or dimethylallylamino purine (2iP). Data are values of mean ± standard error. CYT: F = 67.3, p < 0.001; CCYT: F = 75.2, p < 0.001; and CCYT (CYT): F = 20.6, p < 0.001. DFnum and DFden of CCYT (CYT) were 4 and 27, respectively. Treatments with a letter in common are not different at p > 0.05 according to the Tukey's test applied to the LSMEANS estimates’ differences.

Root number of Cistus crispus microcuttings at increasing concentration (CAUX) of auxin (AUX): indole acetic acid (IAA) or indole butyric acid (IBA). Data are values of mean ± standard error. AUX: F = 11.4, p = 0.003; CAUX: F = 8.6, p = 0.002; CAUX (AUX): F = 0.007, p = 0.935. DFnum and DFden of CAUX (AUX) were 2 and 18, respectively.

Articles in this issue

DOI: https://doi.org/10.2478/fhort-2020-0001 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761

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Language: English

Page range: 1 - 9

Submitted on: Apr 11, 2019

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Accepted on: Nov 12, 2019

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Published on: Jul 27, 2020

Published by: Polish Society for Horticultural Sciences (PSHS)

In partnership with: Paradigm Publishing Services

Publication frequency: 2 issues per year

Keywords:

Mediterranean maquis,

plant hormones,

Related subjects:

Life sciences, other

© 2020 Sergio Saia, Antonio Giovino, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 32 (2020): Issue 1 (June 2020)