Peculiarities of determining the morphogenesis of plants Corylus avellana L. and Prunus dulcis (Mill.) D.A.Webb in vitro culture

Component	Medium composition, mg/l
Component	MS_mod*	QL_mod	DKW_mod	NAM_mod	NRM_mod
NH₄NO₃	1650.0	400.0	1416.0	900	530
KNO₃	1900.0	1800.0	–	250	550
KH₂PO₄	170.0	270.0	265.0	1550	1300
MgSO₄×7H₂O	370.0	360.0	740.0	2050	1650
K₂SO₄	–	–	1600.0	–	–
Ca(NO₃)₂×4H₂O	–	833.8	1365	1050	700
CaCl₂×2H₂O	440.0	–	150	45	90
FeSO₄×7H₂O	27.80		33.8	–
Na₂MoO₄×2H₂O	37.3		45.4	–
Ferrilene 4.8 Orto-Orto	–			114.63
MnSO₄×4H₂O	22.3	0.76	33.5	6.0	20.0
H₃BO₃	6.2		4.80	11.0	6.5
ZnSO₄×7H₂O	8.6		–	11.0	8,8
Zn(NO₃)₂	–		17.0	–	–
CuSO₄×5H₂O	0.025 0.25		0.25	3.2	2.5
Na₂MoO₄×2H₂O	0.025 0.25		0.39	0.1	2.5
CoCl×6H₂O	0.025		–
KJ	0.83	0.08	–
NiSO₄×6H₂O	–		0.0053	–
BAP	1.5 during introduction and multiplication and 0.3 during rhizogenesis
IBA	0.3 during introduction and multiplication and 1.5 during rhizogenesis
Vitamin B₁	1.0
Vitamin B₆	0.6
Vitamin PP	1.0
Vitamin C	3.0
Meso-inositol	100.0
Glycine	1.0
Sucrose/glucose	Sucrose 30 × 10³ almond; glucose 15 × 10³ hazelnut

1 Note: ‘mod’ – means to ‘modified’

Table 2.

Self-intoxication of primary explants depending on the medium and growing conditions of donor plant varieties, %

Variety	Nutrient medium
Variety	MS_mod	QL_mod	DKW_mod	NAM_mod	NRM_mod
Hazelnut
Trapezund	100/16*	98/11	83/4	70/2	24/1
Jefferson	98/19	91/12	77/9	63/1	22/0
Tonda Gentile Romana	100/32	98/26	90/11	66/3	29/2
Almond
E5 Borozan	23/12	6/2	21/18	6/1	8/3
M41 Alex	19/12	5/2	12/9	0/0	7/2
Georgia	14/10	3/1	11/9	0/0	4/0
Louise	8/6	-	6/5	0/0	1/0

1 ^* Numerator: self-intoxication of explants isolated from mother plants grown in the field (control); denominator: grown in the conditions of the depository

Table 3.

The height of regenerants on the 90th day of in vitro cultivation on different nutrient media

Variety	Nutrient medium
	MS_mod	QL_mod	DKW_mod	NAM_mod	NRM_mod
	Height of regenerants, mm
Hazelnut
Trapezund	59/23*	63/27	98/79	86/74	116/91
Jefferson	41/16	58/32	108/87	88/75	109/101
Tonda Gentile Romana	79/29	72/35	111/86	99/70	121/97
Almond
E5 Borozan	84/21	93/73	39/12	123/98	97/69
M41 Alex	78/18	84/71	37/13	134/94	96/66
Georgia	71/13	69/63	33/15	103/84	81/61
Louise	45/12	67/64	23/12	101/85	77/63

1 ^* In the numerator is the height of regenerants during the first cultivation; in the denominator is the height of regenerants during the fifth cultivation.

Table 4.

The height of regenerants on different unloading media on the 90th day of propagation, mm

Variety/media	Nutrient medium
	DKW_mod	ULM*	DKW_mod	NRM_mod	ULM	NRM_mod
	Passage
	5	6	7/12	5	6	7/12
Hazelnut
Trapezund	79	100	111/77	91	109	133/98
Jefferson	87	106	110/83	101	112	139/104
Tonda Gentile Romana	86	113	122/91	97	108	151/ 99
Variety/media	QL_mod	ULM	QL_mod	NAM_mod	ULM	NAM_mod
	Passage
	5	6	7/10	5	6	7/10
Almond
E5 Borozan	73	106	109/65	98	112	114/95
M41 Alex	71	102	106/54	94	116	109/81
Georgia	63	91	98/57	84	94	103/69
Louise	64	97	101/50	85	90	/94/69

1 ^* ULM corresponds to the ‘unloading medium’, which was different in the composition of mineral elements

Table 5.

The height of regenerants on the 90th day of propagation with different selection of passages and cytokinin–auxin index, mm

Variety	Multiplication			Induction of rhizogenesis
	Passage
	5	6	7/10	5	6	7/10
	Cytokinin–auxin index
	1.5/0.3	0.3/1.5	1.5/0.3	0.3/1.5	1.5/0.3	0.3/1.5
Hazelnut
Trapezund	91	114	109/90	109	129	130/99
Jefferson	101	121	97/94	117	132	139/103
Tonda Gentile Romana	97	124	111/96	103	121	132/97
Almond
E5 Borozan	98	103	100/91	102	107	103/104
M41 Alex	94	100	97/89	108	112	107/101
Georgia	84	91	89/83	94	99	97/91
Louise	85	93	86/74	96	97	98/90

Reduction of biometric dimensions of regenerants of the Jefferson variety during long-term cultivation on a medium with an excess of auxins on 30th day of cultivation (the figures correspond to the passage number)

Table 6.

The height of the shoot of regenerants at different concentrations of BAP for fifth, seventh and 10th passages by the overlay passage method, mm

Variety	BAP concentration, mg/l
Variety	0.5	1.0	1.5	2.0	2.5
Hazelnut
Trapezund	92/90/90*	98/94/96	91/83/79	71/66/62	61/51/-
Jefferson	100/102/99	105/107/99	98/95/83	89/76/74	70/65/61
Tonda Gentile Romana	98/97/100	96/98/97	93/88/85	73/58/53	59/52/50
Almond
E5 Borozan	76/74/78	99/98/98	98/64/52	51/43/41	36/-/-
M41 Alex	71/73/75	102/103/96	94/61/52	46/38/36	33/-/-
Georgia	69/66/68	97/98/96	84/55/51	41/32/30	30/22/-
Louise	67/66/64	94/92/95	85/82/56	44/33/33	18/-/-

1 ^* 5/7/10 – indicators in the fifth, seventh and tenth passages

The condition of hazelnut regenerants of the Tonda Gentile Romana variety on a medium with 1.0 mg/l BAP and 0.3 IBA on the 30th day of propagation (the figures correspond to the passage number)

The condition of regenerants of the hazelnut variety Tonda Gentile Romana on a medium with 0.3 mg/l BAP and 1.5 IBA on the 30th day of propagation (the figures correspond to the passage number)

Hyperhydration of hazelnut regenerants of the Tonda Gentile Romana variety during passaging on media with the addition of 2.5 mg/l BAP and 1.5 mg/l gibberellin (the figures correspond to the passage number)

Callus formation *in vitro* in hazelnut and almond explants with the simultaneous addition of high concentrations of cytokinin BAP and IBA to the nutrient medium: (a) callus formation of hazelnut regenerants of the Tonda Gentile Romana variety on the medium with the addition of 2.5 mg/l BAP and 2.5 mg/l IBA; (b) rosette stem of almond regenerants of variety M41 Alex on the medium with the addition of 1.0 mg/l BAP and 1.0 mg/l IBA; (c) rosette stem and callus formation of almond regenerants of variety M41 Alex on the medium with the addition of 1.5 mg/l BAP and 1.5 mg/l IBA; (d) callus formation of almond regenerants of the M41 Alex variety in the basal part of the cutting explant on the medium with the addition of 2.0 mg/l BAP and 2.0 mg/l IBA; (e) callus formation of almonds of the M41 Alex variety over the entire surface of the cutting explant on the medium with the addition of 2.5 mg/l BAP and 2.5 mg/l IBA

Table 7.

Shoot height of regenerants depending on the age of mother plants of explant donors at different periods of passage, mm

Variety	Age of donors, days
Variety	30	45	90	120	180
Hazelnut
Trapezund	35/24/-	49/41/32	97/84/81	112/109/115	116/114/119
Jefferson	36/31/29	41/32/29	98/95/83	123/127/119	122/127/124
Tonda Gentile Romana	39/30/-	44/35/31	93/89/85	126/119/129	129/133/130
Almond
E5 Borozan	28/-/-	54/52/36	98/84/52	101/111/113	143/148/149
M41 Alex	17/-/-	47/43/26	94/81/52	98/95/103	141/146/145
Georgia	-/-/-	36/33/-	84/65/51	89/91/92	103/105/104
Louise	24/-/-	39/37/30	85/72/56	96/99/99	107/109/110

Table 8.

The height of the progeny shoots with different duration of the dormant state of explant donors, mm

Variety	Age of donors, days
Variety	0	45	90	120
Hazelnut
Trapezund	99	114	139	131
Jefferson	98	114	154	152
Tonda Gentile Romana	96	107	133	138
Almond
E5 Borozan	95	102	109	110
M41 Alex	91	100	103	101
Georgia	87	93	108	105
Louise	84	98	112	121

*In vitro* cultivation of Tonda Gentile Romana hazelnut explants during pre-adaptation: (a) coconut; (b) pearlite; (c) a mixture of pearlite and coconut (1:1)

Table 9.

Peculiarities of the photoautotrophic microclonal propagation of hazelnut plants with different pre-adaptation of the Tonda Gentile Romana variety

Indicator	Mediumт
	coconut		pearlite		mixture of pearlite and coconut (1:1)
	Germination conditions
	in vitro	micro greenhouse	in vitro	micro greenhouse	in vitro	micro greenhouse
Survival rate, %	58	67	60	69	69	94
Damaged leaf area, %	12	27	15	19	12	14
The height of plants on the 14th day of growing FMP, mm	189	213	162	184	221	267

References

Authors

Metrics

Articles in this issue

DOI: https://doi.org/10.2478/ffp-2023-0001 | Journal eISSN: 2199-5907 | Journal ISSN: 0071-6677

Journal RSS Feed

Language: English

Page range: 1 - 14

Submitted on: Aug 18, 2022

Accepted on: Nov 18, 2022

Published on: Mar 13, 2023

Published by: Forest Research Institute

In partnership with: Paradigm Publishing Services

Keywords:

cytokinin–auxin index,

explant,

heterotrophic and autotrophic nutrition,

microclonal propagation,

nutrient medium,

regeneration,

self-intoxication with phenol-like substances

Related subjects:

Life sciences,

Plant science

© 2023 Vyacheslav Matskevych, Vasyl Yukhnovskyi, Ivan Kimeichuk, Oksana Matskevych, Oksana Shyta, published by Forest Research Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 65 (2023): Issue 1 (March 2023)

Peculiarities of determining the morphogenesis of plants Corylus avellana L. and Prunus dulcis (Mill.) D.A.Webb in vitro culture

Figures & Tables

Table 1.

Table 2.

Table 3.

Table 4.

Table 5.

Figure 1.

Table 6.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

Table 7.

Table 8.

Figure 6.

Table 9.

Paradigm

My account