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Lelliottia nimipressuralis (Carter 1945) Brady et al. 2013 as the causative agent of bacterial wetwood disease of common silver fir (Abies alba mill.) Cover

Lelliottia nimipressuralis (Carter 1945) Brady et al. 2013 as the causative agent of bacterial wetwood disease of common silver fir (Abies alba mill.)

Folia Forestalia Polonica
Volume 64 (2022): Issue 3 (September 2022)
By:
Open Access
|Sep 2022

Figures & Tables

Figure 1

Macro-signs of bacterial wetwood disease in Abies alba:A – cracks and exudate on rhytidome; B – ulcer formed with exposed secondary phloem and wound meristem (callus); C – wet base of the trunk; D1 – dieback of epicormic shoots; D2 – settlement of secondary pathogens
Macro-signs of bacterial wetwood disease in Abies alba:A – cracks and exudate on rhytidome; B – ulcer formed with exposed secondary phloem and wound meristem (callus); C – wet base of the trunk; D1 – dieback of epicormic shoots; D2 – settlement of secondary pathogens

Figure 2

Wet ulcer on the trunk, under the bark of which dark brown affected areas of wood were found against the background of healthy light-coloured wood – A and affected (as if burned) vessels of the sapwood part of the knot – B
Wet ulcer on the trunk, under the bark of which dark brown affected areas of wood were found against the background of healthy light-coloured wood – A and affected (as if burned) vessels of the sapwood part of the knot – B

Figure 3

Bacterial colonies on potato agar isolated from common silver fir
Bacterial colonies on potato agar isolated from common silver fir

Figure 4

Colonies of the collection strain Erwinia nimipressuralis 8791 on potato agar
Colonies of the collection strain Erwinia nimipressuralis 8791 on potato agar

Figure 5

Cells of the causative agent of bacterial wetwood disease of common silver fir under a light microscope
Cells of the causative agent of bacterial wetwood disease of common silver fir under a light microscope

Figure 6

Characteristics of F5 isolate which were determined by using the NEFERMtest24 MikroLaTE test system
Characteristics of F5 isolate which were determined by using the NEFERMtest24 MikroLaTE test system

Figure 7

Growth of bacterial isolates on glucose medium (anaerobic) using the NEFERMtest24 MikroLaTEST®, ErbaLachema test system
Growth of bacterial isolates on glucose medium (anaerobic) using the NEFERMtest24 MikroLaTEST®, ErbaLachema test system

Figure 8

Characteristics of isolate 11 using API 20E test system
Characteristics of isolate 11 using API 20E test system

Physiological and biochemical properties of isolates fromwooden samples of fir (affection of type a) by using the TNEFERMtest24 MikroLaTEST®, ErbaLachema test system

SymbolTestIsolates from fir (4)
OXIOxidase+
UREUrease+
ARGArginine+
ORNOrnithine+
LYSLysine−
AAMAcetamide+
βGLβ-Glucosidase+
NAGN-acetyl-β-d-glucosamididase−
SCISimpson citrate+
LACLactose−
MANMannitol+
TRETrehalose+
XYLXylose+
ARAArabinose+
αGAα-Galactosidase−
βGAβ-Galactosidase−
MALMalonate+
GALGalactose+
MLTMaltose−
CELCellobiose+
SUCSaccharose+
INOInositol−
γGTγ-Glutamyl transferase+
PHSPhosphatase+
ESLEsculin+
Glucose (anaerobic)−

Morphological and biochemical properties of isolates from fir

TestsBacterial isolatesErwinia nimipressuralis 8791
123
Gram's staining−−
Yellow pigment−−
Oxidase−−
Reduction of nitrates++
Formation of H2S−−
Formation of indole−−
β-Galactosidase++
Arginine dehydrolase−−
Lysine decarboxylase−−
Ornithine decarboxylase++
The use of citrate++
Urease−−
Tryptophan deaminase++
Voges–Proskauer reaction++
Gelatinase−−
Pectinase activity−−
The use of: Glucose (anaerobic)++
Mannitol++
Inositol−−
Sorbitol−−
Rhamnose++
Melibiose++
Amygdalin++
Arabinose++

Physiological and biochemical properties of fir isolates (affection of type a, Fig_ 2) (test system API 20 E)

TestsBacterial isolates
Yellow pigment−
Reduction of nitrates+
Formation of H2S−
Formation of indole−
β-galactosidase−
Arginine dehydrolase+
Lysine decarboxylase+
Ornithine decarboxylase+
The use of citrate+
Urease−
Tryptophan deaminase+
Voges–Proskauer reaction+
Gelatinase+
The use of glucose (anaerobic)−
Mannitol, inositol, sorbitol, rhamnose, saccharose−
Melibiose, amygdalin, arabinose−
DOI: https://doi.org/10.2478/ffp-2022-0017 | Journal eISSN: 2199-5907 | Journal ISSN: 0071-6677
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Language: English
Page range: 173 - 183
Submitted on: Feb 17, 2022
Accepted on: May 9, 2022
Published on: Sep 22, 2022
Published by: Forest Research Institute
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
dieback,
aetiology,
J. Braun-Blanquet method,
pathogenesis
Related subjects:
Life sciences,
Plant science,
Medicine,
Veterinary medicine

© 2022 Ivanna M. Kulbanska, Petro P. Plikhtyak, Maryna V. Shvets, Myroslava I. Soroka, Anatoliy F. Goychuk, published by Forest Research Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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