Figure 1
The effect of growth regulators and conditions of illumination on the induction of callus formation in S_ truncata plant explants, 25 days in in vitro culture
| Variants | The composition of the nutrient medium | Conditions of illumination | |
|---|---|---|---|
| Light room (2.0−3.0 klx) callus formation frequency (mean ± standard error), % | Thermostat (without light) callus formation frequency (mean ± standard error), % | ||
| C | Control | 0 | 0 |
| 1 | MS + 2.0 mg/l 2.4-D | 0 | 33.3±8.8 |
| 2 | MS + 1.0 mg/l BA + 0.5 mg/l NAA | 90.0±5.8 | 93.3±3.3 |
Growth characteristics of S_ truncata plant shoots cultivated on modified nutrient media MS, 60 days in vitro culture
| Variants | The composition of the nutrient medium | The length of one segment section of sprouts (mean ± standard error), cm | Plants multiplication factor (mean ± standard error) | Pigmentation | The presence of the root system |
|---|---|---|---|---|---|
| C | hormones free | 1.0±0.2 | 2.0±0.3 | light green | + |
| 1 | 2.0 mg/l BA | 0.5±0.1 | 10.0±0.7 | light green - | − |
| 2 | 0.5 mg/l kinetin BA + 0.5 mg/l | 1.2±0.2 | 8.8±0.6 | green | + |
| 3 | 1.0 mg/l IBA | 1.1±0.2 | 1.6±0.2 | green | + |
| 4 | 0.25 mg/l kinetin | 1.4±0.1 | 3.8±0.4 | green | + |
| 5 | activated 0.25 mg/carbon l kinetin adsorbent + 2 g/l | 1.5±0.1 | 4.2±0.4 | green | + |