First report of Apiognomonia errabunda on Quercus ilex in Algeria

Oussama Ali Bensaci; Riadh Beghami; Kamel Gouaref

doi:10.2478/ffp-2021-0002

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First report of Apiognomonia errabunda on Quercus ilex in Algeria

Folia Forestalia Polonica

Volume 63 (2021): Issue 1 (March 2021)

By: Oussama Ali Bensaci, Riadh Beghami and Kamel Gouaref

Open Access

|Mar 2021

Figures & Tables

Map depicting the study sites situated in Belezma National Park: BRG: Bordjem, TUG: Tougurt, CHL: Chelaâlaâ, AAL: Ain Ali

Leaves of *Q. ilex* showing characteristic brown-necrosis caused by *D. quercina*

Table 1

Mean severity (%) calculated for each sites according to digital image analyses of Q. ilex leaves affected by anthracnose

Sites	Number of sampled trees	Number of sampled leaves	Severity (%)	Severity index	Horsfall-Barratt Scale
Bordjem	10	374	21.85 ± 0.18 ^b	0.10	3.99 ± 0.18
Tougurt	10	350	34.52 ± 0.15^a	0.13	4.76 ± 0.15
Chelaâlaâ	10	396	21.96 ± 0.17^b	0.11	4.01 ± 0.17
Ain Ali	10	319	13.66 ± 0.22^c	0.03	3.03 ± 0.22

[i] Values represent means ± SE. Means followed by the same letter(s) within the same column are not significantly different (α = 0.05) according to LSD Fisher test.

Table 2

Variability of anthracnose severity (%) on leaves of Q. ilex according to the orientation of twigs

Twig orientation	Bordjem	Tougurt	Chelaâlaâ	Ain Ali
East	20.61 ± 0.39^ab	31.56 ± 0.32^b	21.51 ± 0.35^ab	12.63 ± 0.38^b
West	18.35 ± 0.40^b	26.80 ± 0.34^b	17.53 ± 0.38^b	9.79 ± 0.38^b
North	20.53 ± 0.34^ab	26.64 ± 0.34^b	19.36 ± 0.64^b	13.87 ± 0.51^ab
South	25.79 ± 0.31^a	43.87 ± 0.24^a	26.08 ± 0.28^a	21.60 ± 0.48^a
P	0.0773	<0.0001	0.0075	0.0093

[i] Values represent means ± SE. Means followed by the same letter(s) within the same column are not significantly different (α = 0.05) according to LSD Fisher test.

Table 3

Severity (%) variation of Q. ilex anthracnose according to leaf position on twigs

	Bordjem	Tougurt	Chelaâlaâ	Ain Ali
NAP	20.02 ± 0.22^b	32.93 ± 0.21^a	21.55 ± 0.23^a	14.08 ± 0.28^a
AP	25.43 ± 0.63^a	36.34 ± 0.22^a	22.43 ± 0.25^a	13.07 ± 0.33^a
P	0.0193	0.1347	0.6597	0.6916

[i] Values represent means ± SE. Means followed by the same letter(s) within the same column are not significantly different (α = 0.05) according to LSD Fisher test. NAP: non-apical leaves, AP: apical leaves.

*Apiognomonia errabunda* cultures on MEA medium. (A) AECS3 isolated from Chelaâlaâ. (B) AETS2 isolated from Tougurt. (C) AEBS5 isolated from Brdjem. (D) AEAN6 isolated from Ain Ali

Table 4

Cultural characteristics of the 35 D. quercina isolates on MEA medium

Site	Isolates	Morphotype	Sporulation	Acervuli	(Diam mm)	CUps	CLws	Shape	ConidiaL (μm)	ConidiaW (μm)
Bordjem	DQBS1	M1	S	–	37.00	LB	LB	Circular	9.00	5.00
	DQBS2	M1	S	–	39.33	LB	LB	Circular	11.00	3.00
	DQBS3	M1	S	–	47.00	LB	LB	Circular	9.00	3.00
	DQBS4	M1	S	–	41.67	LB	LB	Circular	10.00	4.00
	DQBS5	M3	S	–	41.00	LB	LB	Circular	9.00	3.00
	DQBS6	M5	S	–	40.00	LB	LB	Circular	6.00	4.00
	DQBN1	M5	NS	–	40.67	LB	LB	Circular	n/a	n/a
Tougurt	DQTS1	M1	S	–	45.67	LB	LB	Circular	7.00	3.00
	DQTS2	M1	S	–	43.33	BG	LG	Circular	8.00	3.00
	DQTS3	M1	S	–	41.67	BG	LG	Circular	6.00	3.00
	DQTN1	M1	NS	–	39.67	LB	LB	Circular	n/a	n/a
	DQTS4 DQTN2	M2 M2	S S	– –	45.33 44.00	LB LB	LB LB	Circular Circular	8.00 7.00	5.00 3.00
	DQTS5	M2	S	–	46.67	LB	LB	Circular	8.00	2.00
	DQTN3	M3	S	–	40.00	LB	LB	Circular	8.00	4.00
	DQTN4	M3	NS	–	41.67	LB	LB	Circular	n/a	n/a
	DQTS6	M5	S	–	47.67	LB	LBCC	Circular	9.00	3.00
Chelaâlaâ	DQCN1	M1	NS	–	41.33	BG	LG	Circular	n/a	n/a
	DQCS1	M3	S	+	43.67	LB	LB	Circular	7.00	4.00
	DQCS2 DQCS3	M4 M5	S S	– –	43.67 43.33	LB BG	LB LG	Circular Circular	9.00 8.00	3.00 4.00
	DQCS4	M5	S	–	42.67	LB	LB	Circular	9.00	3.00
	DQCS5	M5	S	–	39.00	LB	LB	Circular	9.00	4.00
Ain Ali	DQAN1	M1	S	–	33.00	LB	LB	Circular	11.00	3.00
	DQAN2	M1	S	–	42.33	LB	LB	Circular	11.00	4.00
	DQAN3	M4	S	+	43.33	LB	LB	Circular	9.00	3.00
	DQAS1	M1	S	–	35.33	LB	LB	Circular	8.00	4.00
	DQAS2	M1	S	–	45.67	LB	LBCC	Circular	8.00	3.00
	DQAS3 DQAN4	M1 M1	S NS	– –	41.00 41.33	LB LB	LB LB	Circular Circular	9.00 n/a	4.00 n/a
	DQAN5	M1	S	+	49.67	BG	LG	Circular	6.00	3.00
	DQAS4	M2	S	+	50.00	BG	LG	Circular	7.00	3.00
	DQAN6	M2	NS	–	46.00	LB	LB	Circular	n/a	n/a
	DQAS5	M3	S	–	40.00	LB	LB	Circular	7.00	3.00
	DQAS6	M3	S	–	50.67	LB	LB	Circular	7.00	4.00

[i] CSup: colony color in the upper side of Petri dish. CLws: colony color in the lower side of Petri dish. ConidiaL: conidia length. ConidiaW: conidia width. n/a: not-applicable. NS: non-sporulating isolate. S: sporulating isolate. (+) presence. (-) absence. LB: light brown. BG: brownish-gray. LBCC: light brown with gray concentric circles. LG: light gray.

Phylogenetic tree (rDNA ITS) of *A. errabunda* including the two isolates from *Q. ilex* in Belezma National Park. Dendrogram was elaborated implying the Neighbor-Joining method based on the Jukes-Cantor model. The bootstrap tree inferred from 1000 replicates (The optimal tree with the sum of branch length = 0.62132144). Two accessions of *Ophiostoma novo-ulmi* were considered in this tree as out-group taxa

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DOI: https://doi.org/10.2478/ffp-2021-0002 | Journal eISSN: 2199-5907 | Journal ISSN: 0071-6677

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Language: English

Page range: 10 - 20

Submitted on: Apr 10, 2019

Accepted on: Nov 9, 2020

Published on: Mar 13, 2021

Published by: Polish Academy of Sciences, Committee on Forest Sciences and Wood Technology and Forest Research Institute

In partnership with: Paradigm Publishing Services

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© 2021 Oussama Ali Bensaci, Riadh Beghami, Kamel Gouaref, published by Polish Academy of Sciences, Committee on Forest Sciences and Wood Technology and Forest Research Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 63 (2021): Issue 1 (March 2021)

First report of Apiognomonia errabunda on Quercus ilex in Algeria

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Table 1

Table 2

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