First report of Apiognomonia errabunda on Quercus ilex in Algeria

Oussama Ali Bensaci; Riadh Beghami; Kamel Gouaref

doi:10.2478/ffp-2021-0002

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First report of Apiognomonia errabunda on Quercus ilex in Algeria

Folia Forestalia Polonica

Volume 63 (2021): Issue 1 (March 2021)

By: Oussama Ali Bensaci, Riadh Beghami and Kamel Gouaref

Open Access

|Mar 2021

Figures & Tables

Map depicting the study sites situated in Belezma National Park: BRG: Bordjem, TUG: Tougurt, CHL: Chelaâlaâ, AAL: Ain Ali

Leaves of Q. ilex showing characteristic brown-necrosis caused by D. quercina

Apiognomonia errabunda cultures on MEA medium. (A) AECS3 isolated from Chelaâlaâ. (B) AETS2 isolated from Tougurt. (C) AEBS5 isolated from Brdjem. (D) AEAN6 isolated from Ain Ali

Phylogenetic tree (rDNA ITS) of A. errabunda including the two isolates from Q. ilex in Belezma National Park. Dendrogram was elaborated implying the Neighbor-Joining method based on the Jukes-Cantor model. The bootstrap tree inferred from 1000 replicates (The optimal tree with the sum of branch length = 0.62132144). Two accessions of Ophiostoma novo-ulmi were considered in this tree as out-group taxa

Severity (%) variation of Q_ ilex anthracnose according to leaf position on twigs

	Bordjem	Tougurt	Chelaâlaâ	Ain Ali
NAP	20.02 ± 0.22^b	32.93 ± 0.21^a	21.55 ± 0.23^a	14.08 ± 0.28^a
AP	25.43 ± 0.63^a	36.34 ± 0.22^a	22.43 ± 0.25^a	13.07 ± 0.33^a
P	0.0193	0.1347	0.6597	0.6916

Cultural characteristics of the 35 D_ quercina isolates on MEA medium

Site	Isolates	Morphotype	Sporulation	Acervuli	(Diam mm)	CUps	CLws	Shape	ConidiaL (μm)	ConidiaW (μm)
Bordjem	DQBS1	M1	S	–	37.00	LB	LB	Circular	9.00	5.00
	DQBS2	M1	S	–	39.33	LB	LB	Circular	11.00	3.00
	DQBS3	M1	S	–	47.00	LB	LB	Circular	9.00	3.00
	DQBS4	M1	S	–	41.67	LB	LB	Circular	10.00	4.00
	DQBS5	M3	S	–	41.00	LB	LB	Circular	9.00	3.00
	DQBS6	M5	S	–	40.00	LB	LB	Circular	6.00	4.00
	DQBN1	M5	NS	–	40.67	LB	LB	Circular	n/a	n/a
Tougurt	DQTS1	M1	S	–	45.67	LB	LB	Circular	7.00	3.00
	DQTS2	M1	S	–	43.33	BG	LG	Circular	8.00	3.00
	DQTS3	M1	S	–	41.67	BG	LG	Circular	6.00	3.00
	DQTN1	M1	NS	–	39.67	LB	LB	Circular	n/a	n/a
	DQTS4 DQTN2	M2 M2	S S	– –	45.33 44.00	LB LB	LB LB	Circular Circular	8.00 7.00	5.00 3.00
	DQTS5	M2	S	–	46.67	LB	LB	Circular	8.00	2.00
	DQTN3	M3	S	–	40.00	LB	LB	Circular	8.00	4.00
	DQTN4	M3	NS	–	41.67	LB	LB	Circular	n/a	n/a
	DQTS6	M5	S	–	47.67	LB	LBCC	Circular	9.00	3.00
Chelaâlaâ	DQCN1	M1	NS	–	41.33	BG	LG	Circular	n/a	n/a
	DQCS1	M3	S	+	43.67	LB	LB	Circular	7.00	4.00
	DQCS2 DQCS3	M4 M5	S S	– –	43.67 43.33	LB BG	LB LG	Circular Circular	9.00 8.00	3.00 4.00
	DQCS4	M5	S	–	42.67	LB	LB	Circular	9.00	3.00
	DQCS5	M5	S	–	39.00	LB	LB	Circular	9.00	4.00
Ain Ali	DQAN1	M1	S	–	33.00	LB	LB	Circular	11.00	3.00
	DQAN2	M1	S	–	42.33	LB	LB	Circular	11.00	4.00
	DQAN3	M4	S	+	43.33	LB	LB	Circular	9.00	3.00
	DQAS1	M1	S	–	35.33	LB	LB	Circular	8.00	4.00
	DQAS2	M1	S	–	45.67	LB	LBCC	Circular	8.00	3.00
	DQAS3 DQAN4	M1 M1	S NS	– –	41.00 41.33	LB LB	LB LB	Circular Circular	9.00 n/a	4.00 n/a
	DQAN5	M1	S	+	49.67	BG	LG	Circular	6.00	3.00
	DQAS4	M2	S	+	50.00	BG	LG	Circular	7.00	3.00
	DQAN6	M2	NS	–	46.00	LB	LB	Circular	n/a	n/a
	DQAS5	M3	S	–	40.00	LB	LB	Circular	7.00	3.00
	DQAS6	M3	S	–	50.67	LB	LB	Circular	7.00	4.00

Mean severity (%) calculated for each sites according to digital image analyses of Q_ ilex leaves affected by anthracnose

Sites	Number of sampled trees	Number of sampled leaves	Severity (%)	Severity index	Horsfall-Barratt Scale
Bordjem	10	374	21.85 ± 0.18 ^b	0.10	3.99 ± 0.18
Tougurt	10	350	34.52 ± 0.15^a	0.13	4.76 ± 0.15
Chelaâlaâ	10	396	21.96 ± 0.17^b	0.11	4.01 ± 0.17
Ain Ali	10	319	13.66 ± 0.22^c	0.03	3.03 ± 0.22

Variability of anthracnose severity (%) on leaves of Q_ ilex according to the orientation of twigs

Twig orientation	Bordjem	Tougurt	Chelaâlaâ	Ain Ali
East	20.61 ± 0.39^ab	31.56 ± 0.32^b	21.51 ± 0.35^ab	12.63 ± 0.38^b
West	18.35 ± 0.40^b	26.80 ± 0.34^b	17.53 ± 0.38^b	9.79 ± 0.38^b
North	20.53 ± 0.34^ab	26.64 ± 0.34^b	19.36 ± 0.64^b	13.87 ± 0.51^ab
South	25.79 ± 0.31^a	43.87 ± 0.24^a	26.08 ± 0.28^a	21.60 ± 0.48^a
P	0.0773	<0.0001	0.0075	0.0093

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DOI: https://doi.org/10.2478/ffp-2021-0002 | Journal eISSN: 2199-5907 | Journal ISSN: 0071-6677

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Language: English

Page range: 10 - 20

Submitted on: Apr 10, 2019

Accepted on: Nov 9, 2020

Published on: Mar 13, 2021

Published by: Forest Research Institute

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

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© 2021 Oussama Ali Bensaci, Riadh Beghami, Kamel Gouaref, published by Forest Research Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 63 (2021): Issue 1 (March 2021)

First report of Apiognomonia errabunda on Quercus ilex in Algeria

Figures & Tables

Figure 1

Figure 2

Figure 3

Figure 4

Severity (%) variation of Q_ ilex anthracnose according to leaf position on twigs

Cultural characteristics of the 35 D_ quercina isolates on MEA medium

Mean severity (%) calculated for each sites according to digital image analyses of Q_ ilex leaves affected by anthracnose

Variability of anthracnose severity (%) on leaves of Q_ ilex according to the orientation of twigs

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