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Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia Cover

Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia

Open Access
|Dec 2019

Abstract

Conservation of bamboos for future exploitation as fuel, fibre and as an ingredient for cosmetics depends on knowledge of its natural genetic variation. The study of molecular genetic diversity in bamboos will provide important information for its conservation. This article reports on the genetic diversity in 25 species representing five genera of bamboos found in Indonesia using Random Amplified Polymorphic DNA (RAPD) molecular markers. Out of 40 primers, 24 primers produced 1107 total bands and 86.21% of polymorphic bands across the 25 species. Sixteen bands were uniquely found in one species only and their presence or absence helped to define nine bamboo species. RAPD band sizes ranged from 162 to 2247 base pairs. A dendrogram based on the similarity coefficient of Dice divided the bamboo species into three big clusters. In conclusion, RAPD can capture the diversity among five different bamboo genera and has a great potential to be used in the study of genetic diversity in Indonesian bamboos.

DOI: https://doi.org/10.2478/ffp-2019-0025 | Journal eISSN: 2199-5907 | Journal ISSN: 0071-6677
Language: English
Page range: 255 - 266
Submitted on: Mar 5, 2019
Accepted on: Aug 5, 2019
Published on: Dec 30, 2019
Published by: Forest Research Institute
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2019 Annisa, Rini Hafzari, Tia Setiawati, Budi Irawan, Joko Kusmoro, published by Forest Research Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.