Activity of spore-crystal mixtures of new Bacillus thuringiensis strains against Dendrolimus pini (Lepidoptera: Lasiocampidae) and Spodoptera exigua (Lepidoptera: Noctuidae)

Konecka, Edyta; Kaznowski, Adam; Stachowiak, Małgorzata; Maciąg, Mirosław

doi:10.2478/ffp-2018-0009

Abstract

We estimated the usefulness of spore-crystals preparations of the two B. thuringiensis isolates, MPU B9 and MPU B54, for reducing the number of pests. The potential insecticidal toxicities of B. thuringiensis isolates were assessed by the analysis of the genes coding for crystalline proteins. The activities of spore-crystals preparations were determined against Dendrolimus pini L. (Lepidoptera: Lasiocampidae) and compared with the toxicity of spores and crystals of B. thuringiensis subsp. kurstaki HD-1 from commercial biopesticide Foray. Although the analysis of crystalline toxin gene profiles indicated potentially higher activities of MPU B9 and MPU B54 crystals against the pests than that of HD-1, the toxicities of isolate and HD-1 preparations against D. pini caterpillars were similar. The LC₅₀ amounted to 3.42×10⁴ spores and crystals for HD-1, 3.36×10⁴ for MPU B9 and 3.5×10⁴ for MPU B54. Additionally, the toxicity of the MPU B54 preparation was evaluated against Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae). The LC₅₀ was 4.5×10⁵ spores and crystals of MPU B54, and 2.69×10⁶ spores and crystals of HD-1. The LC₅₀ of the MPU B54 preparation against S. exigua was approximately six-fold higher than that of HD-1. However, due to the very wide fiducidal limits for LC₅₀ values, which for both preparations overlap to a large extent, the toxicity of the preparations should be considered the same. The varied profiles of crystalline toxin genes and important toxicity of spore-crystal mixtures of isolates against S. exigua and D. pini indicate the effectiveness of the mixtures against pests and make the strains an alternative for HD-1 for reducing the number of insects.

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Activity of spore-crystal mixtures of new Bacillus thuringiensis strains against Dendrolimus pini (Lepidoptera: Lasiocampidae) and Spodoptera exigua (Lepidoptera: Noctuidae)

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