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pH of prepared microemulsion batches_
| Batch no. | pH |
|---|---|
| RME1 | 5.24 |
| RME2 | 5.93 |
| RME3 | 5.67 |
Ingredients list for preparation of FU microemulsion_
| Ingredient | Property |
|---|---|
| 5-FU | Drug |
| Tween 20 | Surfactant |
| Isopropyl alcohol | Cosurfactant |
| Isopropyl myristate | Oil |
| DMSO | Penetration enhancer |
| Rutin | Antioxidant |
Values of drug permeation of prepared microemulsion batches_
| Time | 5-FU | RME1 | RME2 | RME3 |
|---|---|---|---|---|
| Absorption | Absorption | Absorption | Absorption | |
| 0 min | 0.128 | 0.128 | 0.135 | 0.138 |
| 1 min | 0.130 | 0.145 | 0.152 | 0.156 |
| 2 min | 0.161 | 0.165 | 0.174 | 0.178 |
| 3 min | 0.182 | 0.193 | 0.203 | 0.208 |
| 4 min | 0.217 | 0.232 | 0.244 | 0.251 |
| 5 min | 0.275 | 0.291 | 0.305 | 0.312 |
| 10 min | 0.358 | 0.386 | 0.406 | 0.416 |
| 30 min | 0.510 | 0.581 | 0.61 | 0.625 |
| 60 min | 1.012 | 1.161 | 1.22 | 1.25 |
| 3 hours | 1.012 | 1.161 | 1.22 | 1.25 |
Solubility of R-5FU in different oils, surfactants and co-surfactants_
| Oil | Solubility(mg/ml) |
| Isopropyl myristate | 165.28 |
| Oleic acid | 183.12 |
| Castor oil | 44.26 |
| Olive oil | 32.61 |
| Surfactant | Solubility (mg/ml) |
| Tween 80 | 105.28 |
| Tween 20 | 93.12 |
| Span 80 | 58.27 |
| Span 20 | 31.62 |
| Cosurfactant | Solubility (mg/ml) |
| Isopropyl alcohol | 107.28 |
| Ethanol | 43.12 |
| N-butanol | 58.27 |
| Polyethylene glycol | 69.62 |
| Propylene glycol | 79.62 |
Scoring for irritation testing (HET-CAM assay)_
| Test formulation | Effect | Score | Net score | Inference | ||
|---|---|---|---|---|---|---|
| 0.5 min | 2 min | 5 min | ||||
| RME1 | Lysis | -- | -- | -- | 0 | Non-irritant |
| Hemorrhage | -- | -- | -- | |||
| Coagulation | -- | -- | -- | |||
| RME2 | Lysis | - | -- | -- | 0 | Non-irritant |
| Hemorrhage | -- | -- | -- | |||
| Coagulation | -- | -- | -- | |||
| RME3 | Lysis | -- | -- | -- | 0 | Non-irritant |
| Hemorrhage | -- | -- | -- | |||
| Coagulation | -- | -- | -- | |||
| Control (0.9%NaCl) | Lysis | -- | -- | -- | 0 | non-irritant |
| Hemorrhage | -- | -- | -- | |||
| Coagulation | -- | -- | -- | |||
| Irritant (0.1 N NaOH) | Lysis | -- | 7 | -- | 23 | Severe-irritant |
| Hemorrhage | 7 | -- | -- | |||
| Coagulation | 9 | -- | -- | |||
Effects of RME1, RME2, RME3, control and irritant on chorioallantoic membrane with different time interval_
| Time: - | 0.5 minutes | 2 minutes | 5 minutes |
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| Groups: - | |||
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| RME2 |
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| RME3 |
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Mean erythemal score evaluated for different formulation_
| Erythemal Score (n-6) | |||||||
|---|---|---|---|---|---|---|---|
| Days | 2 | 5 | 7 | 10 | 14 | 18 | 21 |
| Control | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| RME1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| RME2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
| RME3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
| Formalin (0.8%) | 0 | 0 | 1 | 1 | 2 | 3 | 3 |
Viscosity of prepared microemulsion batches_
| Batch no. | Viscosity |
|---|---|
| RME1 | 14.68 |
| RME2 | 16.52 |
| RME3 | 17.25 |