Figure 1

Study characteristics_
| Study groups | Average Age (in years) | Gender | Site | Staging | |||||
|---|---|---|---|---|---|---|---|---|---|
| Male | Female | Gingivo-buccal sulcus | Alveolus | Tongue | Buccal mucosa | III | IV | ||
| OSCC with lymph node metastasis | 52.92 | 16 | 9 | 8 | 10 | 3 | 4 | 18 | 7 |
| OSCC without lymph node metastasis | 53.88 | 17 | 8 | 9 | 11 | 2 | 3 | 19 | 6 |
Association between staining intensity and lymph node status_
| Staining Intensity | Lymph node status | p-value | ||
|---|---|---|---|---|
| N1 | N2 | N3 | ||
| Negative | 7 | 0 | 0 | 0.001* |
| Positive, well differentiated | 4 | 4 | 3 | |
| Positive Moderately Differentiated | 0 | 3 | 4 | |
Association between staining intensity and lymph node involvement_
| Staining Intensity | Lymph node involvement | p-value | |
|---|---|---|---|
| Positive | Negative | ||
| Negative | 7 | 20 | 0.0005* |
| + Positive | 12 | 2 | |
| ++ Positive | 6 | 3 | |
| +++ Positive | 0 | 0 | |