Implantation and pregnancy outcome of Sprague-Dawley rats exposed to pirimiphos-methyl

Oyesola, Tolulope; Iranloye, Bolanle; Adegoke, Olufeyi

doi:10.2478/enr-2019-0014

Abstract

Objective. This study was designed to investigate the effect of sublethal doses (10, 60, and 120 mg/kg of pirimiphos-methyl on implantation and pregnancy in female Sprague-Dawley rats. Pirimiphos-methyl is a pesticide widely used worldwide, especially in Africa to protect food against pests and has gained widespread acceptance.

Methods. Pregnant Sprague-Dawley rats used for this study had access to food and water ad libitum and were divided into a control group and three experimental groups based on dose of chemical given. The pregnant rats were given pirimiphos-methyl orally on days 1–5, 1–7, 7–18^th day of gestation and from day 1 to term. Implantation studies were carried out on days 6 and 8 of pregnancy, while the fetal parameters were ascertained on day 19 of pregnancy and at term. Serum levels of progesterone and estradiol were measured on days 6, 8 and 19 of pregnancy.

Results. Sublethal administration of pirimiphos-methyl showed decreased number of implantation sites on days 6 and 8, fetal weight, crown-to-rump length, length of umbilical cord and placenta weight (day 19), birth weight, litter size and total number (at term) in rats administered with pirimiphos-methyl when compared with control.

Conclusion. Administration of pirimiphos-methyl resulted in a reduced implantation rate due to decreased uterine receptivity caused by an imbalance in the level of estradiol and progesterone and impaired reproductive outcome during pregnancy.

References

Baldi I, Mohammed-Brahim B, Brochard P, Dartigues JF, Salamon R. Delayed health effects of pesticides: review of current epidemiological knowledge [in French]. Rev Epidemiol Sante Publique 46, 134–142, 1998.
Search in Google Scholar Back to article
Berkowitz GS, Obel J, Deych E, Lapinski R, Godbold J, Liu Z, Landrigan PJ, Wolff MS. Exposure to indoor pesticides during pregnancy in a multiethnic, urban cohort. Environ Health Perspect 111, 79–84, 2003.10.1289/ehp.5619
Search in Google Scholar Back to article
Barron S, Foss J, Riley E. The Effect of prenatal cocaine exposure on umbilical cord length in fetal rats. Neurotoxicol Teratol 13, 503–506, 1991.175840310.1016/0892-0362(91)90057-4
Open DOI Search in Google Scholar Back to article
Bradman A, Barr DB, Claus Henn BG, Drumheller T, Curry C, Eskenazi B. Measurement of pesticides and other toxicants in amniotic fluid as a potential biomarker of prenatal exposure. Environ Health Perspect 111, 1779–1782, 2003.10.1289/ehp.6259124172314594631
Search in Google Scholar Back to article
Cabello G, Valenzuela M, Vilaxa A, Duran V, Rudolph I, Hrepic N, Calaf G. A rat mammary tumor model induced by the organophosphorous pesticides parathion and malathion, possibly through acetylcholinesterase inhibition. Environ Health Perspect 109, 471–479, 2001.10.1289/ehp.01109471124030611401758
Search in Google Scholar Back to article
Carson DD, Bagchi I, Dey SK, Enders AC, Fazleabas AT, Lessey BA, Koshinaga K. Embryo implantation. Dev Biol 223, 217–237, 2000.10.1006/dbio.2000.976710882512
Search in Google Scholar Back to article
Chahoud I, Paumgartten FJR. Relationships between fetal body weight of Wistar rats at term and the extent of skeletal ossification. Braz J Med Biol Res 38, 565–575, 2005.10.1590/S0100-879X20050004000101596218215962182
Open DOI Search in Google Scholar Back to article
Dabrowski S, Hanke W, Polanska K, Makowiec-Dabrowska T, Sobala W. Pesticide exposure and birthweight: an epidemiological study in Central Poland. Int J Occup Med Environ Health 16, 31–39, 2003.
Search in Google Scholar Back to article
Deb K, Reese J, Paria BC. Methodologies to study implantation in mice. Methods Mol Med 121, 9–34, 2006.
Search in Google Scholar Back to article
Desforges M, Sibley C. Placental nutrient supply and fetal development. Int J Dev Biol 54, 377–390, 2010.1987683610.1387/ijdb.082765md19876836
Open DOI Search in Google Scholar Back to article
Dey SK, Lim H, Das SK, Reese J, Paria BC, Daikoku T. Wang H. Molecular cues to implantation. Endocr Rev 25, 341–373, 2004.1518094810.1210/er.2003-002015180948
Open DOI Search in Google Scholar Back to article
Eskenazi B, Bradman A, Castorina R. Exposure of children to organophosphate pesticides and their potential adverse health effects. Environ Health Perspect l07, 409–419, 1999.10.1289/ehp.99107s3409156622210346990
Search in Google Scholar Back to article
Fenster L, Coye MJ. Birthweight of infants born to Hispanic women employed in agriculture. Arch Environ Health 45, 46–52, 1990.231708910.1080/00039896.1990.99359242317089
Open DOI Search in Google Scholar Back to article
Furlong CE, Holland N, Richter RJ, Bradman A, Ho A, Eskenazi B. PON1 status of farmworker mothers and children as a predictor of organophosphate sensitivity. Pharmacogenet Genomics 16, 183–190, 2006.10.1097/01.fpc.0000189796.21770.d316495777
Search in Google Scholar Back to article
Gallo MA, Lawryk NJ. Organic phosphorus pesticides. In Handbook of Pesticide Toxicology. Hayes WJJr. and Laws ER (Eds.), Academic Press, New York. 1991.
Search in Google Scholar Back to article
Grether JK, Harris JA, Neutra R, Kizer KW. Exposure to aerial malathion application and the occurrence of congenital anomalies and low birthweight. Am J Public Health 77, 1009–1010, 1987.10.2105/AJPH.77.8.1009
Search in Google Scholar Back to article
Guney M, Oral B, Demirin H, Take G, Giray SG, Altuntas I Mungan T. Fallopian damage induced by organophosphate insecticide methyl parathion, and protective effect of vitamins E and C on ultrastructural changes in rats. Toxicol Ind Health 23, 428–439, 2007.10.1177/074823370707677318536495
Open DOI Search in Google Scholar Back to article
Hamid HY, Zakaria MA, Yimer N, Meng GY, Haron AW, Mustapha NM. Effects of elevated ambient temperature on embryo implantation in rats. Afri J Biotechnol 11, 6624–6632, 2012.10.5897/AJB11.3228
Search in Google Scholar Back to article
Hayes J, Laws E. Handbook of Pesticide Toxicology. Academic Press, San Diego. 1991.
Search in Google Scholar Back to article
Holland N, Furlong C, Bastaki M, Richter R, Bradman A, Huen K. Paraoxonase polymorphisms, haplotypes, and enzyme activity in Latino mothers and newborns. Environ Health Perspect 114, 985–999, 2006.10.1289/ehp.8540151332216835048
Search in Google Scholar Back to article
Iranloye B, Oyeusi K. Alada A. Effect of aqueous extract of Phyllanthus amarus leaf on implantation and pregnancy in rats. Niger J Physiol Sci 25, 63–66, 2010.
Search in Google Scholar Back to article
Iranloye BO, Oyesola TO. The organophosphate - pirimiphos-methyl alters estrous cycle and hormone concentrations in female Sprague-Dawley rats. World Journal of Pharmaceutical and Life Sciences 4, 207–211, 2018.
Search in Google Scholar Back to article
Koch D, Lu C, Fisker-Andersen J, Jolley L, Fenske R. Temporal association of children’s pesticide exposure and agricultural spraying: report of a longitudinal biological monitoring study. Environ Health Perspect 110, 829–833, 2002.10.1289/ehp.02110829124095712153767
Search in Google Scholar Back to article
Krakowiak P, Smith EN, de BG, Lydon-Rochelle MT. Risk factors and outcomes associated with a short umbilical cord. Obstet Gynecol 103, 119–27, 2004.10.1097/01.AOG.0000102706.84063.C714704255
Search in Google Scholar Back to article
Lesage J, Del-Favero F, Leonhardt M, Louvart H, Maccari S, Vieau D, Darnaudery M. Prenatal stress induces intrauterine growth restriction and programmes glucose intolerance and feeding behaviour disturbances in the aged rat. J Endocrinol 181, 291–296, 2004.10.1677/joe.0.181029115128277
Search in Google Scholar Back to article
Mendoza-Rodriguez CA, Merchant-Larios H, Segura-Valdez ML, Moreno-Mendoza N, Cruz ME, Arteaga-Lopez P, Camacho-Arroyo I, Dominguez R, Cerbon M. c-fos and estrogen receptor gene expression pattern in the rat uterine epithelium during the estrous cycle. Mol Reprod Dev 64, 379–388, 2003.10.1002/mrd.1027712589649
Open DOI Search in Google Scholar Back to article
Ngoula F, Watcho P, Dongmo MC, Kenfack A, Kamtchouing P, Tchoumboue J. Effects of pirimiphos-methyl (an organophosphate insecticide) on the fertility of adult male rats. Afr Health Sci 7, 3–9, 2007.
Search in Google Scholar Back to article
Olaya C, Bernal JE. Clinical associations to abnormal umbilical cord length in Latin American newborns. J Neonatal Perinatal Med 8, 251–256, 2015.10.3233/NPM-1591505626485559
Search in Google Scholar Back to article
Paria BC, Huet H, Dey SK. Blastocyst’s state of activity determines the “window” of implantation in the receptive mouse uterus. Proc Natl Acad Sci U S A 90, 10159–10162, 1993.10.1073/pnas.90.21.10159477338234270
Search in Google Scholar Back to article
Pope CN. Organophosphorous pesticides: do they all have the same mechanism of toxicity? J Toxicol Environ Health B Crit Rev 2, 161–181, 1999.10.1080/10937409928120510230392
Open DOI Search in Google Scholar Back to article
Recio R, Ocampo-Gomez G, Moran-Martinez J, Borja-Aburto V, Lopez-Cervantes M, Uribe M, Torres-Sanchez L, Cebrian ME. Pesticide exposure alters follicle-stimulating hormone levels in Mexican agricultural workers. Environ Health Perspect 113, 1160–1163, 2005.10.1289/ehp.7374128039516140621
Search in Google Scholar Back to article
Sanborn MD, Cole D, Abelsohn A, Weir E. Identifying and managing adverse environmental health effects: 4. Pesticides Can Med Assoc J 166, 1431–1436, 2002.
Search in Google Scholar Back to article
Tamura H, Maness SC, Reishman K, Dorman DC, Gray LE, Gaido KW. Androgen receptor antagonism by the organophosphate insecticide fenilrothion. Toxicol Sci 60, 56–62, 2001.10.1093/toxsci/60.1.5611222873
Open DOI Search in Google Scholar Back to article
Thomas D, Petitti D, Goldhaber M, Swan S, Rappaport E, Hertz-Picciotto I. Reproductive outcomes in relation to malathion spraying in the San Francisco Bay area, 1981–1982. Epidemiology 3, 32–39, 1992.10.1097/00001648-199201000-000071554808
Search in Google Scholar Back to article
Wang H, Dey SK. Roadmap to embryo implantation: clues from mouse models. Nat Rev Genet 7, 185–199, 2006.10.1038/nrg18081648501816485018
Open DOI Search in Google Scholar Back to article
Xiang H, Nuckols JR, Stallones LA. Geographic information assessment of birth weight and crop production patterns around mother’s residence. Environ Res 82, 160–167, 2000.1066253010.1006/enrs.1999.400910662530
Open DOI Search in Google Scholar Back to article
Zhao LH, Cui XZ, Yuan HJ, Liang B, Zheng LL, Liu YX, Luo MJ. Tan JH. Restraint stress inhibits mouse implantation: temporal window and the involvement of HB-EGF, estrogen and progesterone. PLoS One 8, e80472, 2013.2424468910.1371/journal.pone.0080472382824424244689
Search in Google Scholar Back to article

Implantation and pregnancy outcome of Sprague-Dawley rats exposed to pirimiphos-methyl

Abstract

Paradigm

My account