Resistance training alone or combined with leucine supplementation improves the serum lipid profile of diabetic rats, whereas leucine alone does not

Martins, Carlos Eduardo C.; de Sousa Lima, Vanessa B.; Ribeiro, Henrique Quintas T.; Tirapegui, Julio

doi:10.2478/enr-2018-0018

Abstract

Objectives. Diabetes mellitus is associated with dyslipidemia, which contributes to a higher risk of thrombosis, atherosclerosis and cardiovascular disease. This study evaluated the effects of leucine and resistance training on the serum lipid profile in rats with streptozotocin-induced diabetes for 8 weeks.

Methods. Wistar rats with neonatal streptozotocin-induced diabetes were treated with leucine supplementation (5%) and/or resistance training (3 days per week) for 8 weeks, and divided in DL (diabetic and leucine), DT (diabetic and resistance training group) and DLT (diabetic, leucine and resistance training) groups. Others 2 groups of animals received isonitrogen AIN-93M diet that was defined as a control diet: group D (diabetic untreated) and group C (non-diabetic).

Results. The decrease in serum total cholesterol and increase in high-density lipoprotein cholesterol (HDL-C) was observed in the resistance training-induced diabetic rats when compared with diabetic rats. There was no change in serum lipid profile in leucine-supplemented diabetic rats and no synergistic effect of leucine and resistance training. The fasting glucose levels were reduced in all animals treated compared to D group.

Conclusion. The diabetic trained rats demonstrate a protective effect of resistance training on the serum lipid profile.

References

Bierman EL, Amaral JA, Belknap BH. Hyperlipidemia and diabetes mellitus. Diabetes 15, 675–679, 1966.10.2337/diab.15.9.6755916991
Open DOI Search in Google Scholar Back to article
Cartee GD. Mechanisms for greater insulin-stimulated glucose uptake in normal and insulin resistant skeletal muscle after acute exercise. Am J Physiol Endocrinol Metab 309, E949–E959, 2015.10.1152/ajpendo.00416.2015481620026487009
Search in Google Scholar Back to article
Cauza E, Hanusch-Enserer U, Strasser B, Ludvik B, Metz-Schimmerl S, Pacini G, Wagner O, Georg P, Prager R, Kostner K, Dunky A, Haber P. The relative benefits of endurance and strength training on the metabolic factors and muscle function of people with type 2 diabetes mellitus. Arch Phys Med Rehabil 86, 1527–1533, 2005.10.1016/j.apmr.2005.01.00716084803
Open DOI Search in Google Scholar Back to article
Costa RR, Lima AC, Tagliari M, Martins KLF. Effects of resistance training on the lipid profile in obese women. J Sports Med Phys Fitness 51, 169–177, 2011.
Search in Google Scholar Back to article
D’Souza DM, Al-Sajee D, Hawke TJ. Diabetic myopathy: impact of diabetes mellitus on skeletal muscle progenitor cells. Front Physiol 20, 379, 2013.10.3389/fphys.2013.00379386894324391596
Search in Google Scholar Back to article
Donato JJr, Pedrosa RG, Cruzat VF, Pires IS, Tirapegui J. Effects of leucine supplementation on the body composition and protein status of rats submitted to food restriction. Nutrition 22, 520–527, 2006.10.1016/j.nut.2005.12.0081660081716600817
Open DOI Search in Google Scholar Back to article
Durak EP, Jovanovic-Peterson L, Peterson CM. Randomized crossover study of effect of resistance training on glycemic control, muscular strength, and cholesterol in type I diabetic men. Diabetes Care 13, 1039–1043, 1990.10.2337/diacare.13.10.103922093002209300
Open DOI Search in Google Scholar Back to article
Fahlman MM, Boardley D, Lambert CP, Flynn MG. Effects of endurance training and resistance training on plasma lipoprotein profiles in elderly women. J Gerontol A Biol Sci Med Sci 57, B54–B60, 2002.10.1093/gerona/57.2.B5411818424
Open DOI Search in Google Scholar Back to article
Gengiah K, Hari R, Anbu J. Antidiabetic antihyperlipidemic and hepato-protective effect of Gluconorm-5: A polyherbal formulation in steptozotocin induced hyperglycemic rats. Anc Sci Life 34, 23–32, 2014.10.4103/0257-7941.150773434264525737607
Search in Google Scholar Back to article
Ghanbari-Niaki A, Saghebjoo M, Hedayati M. A single session of circuit-resistance exercise effects on human peripheral blood lymphocyte ABCA1 expression and plasma HDL-C level. Regul Pept 166, 42–47, 2011.10.1016/j.regpep.2010.08.00120727372
Search in Google Scholar Back to article
Guido S, Joseph T. Effect of chemically different calcium antagonists on lipid profile in rats fed on a high fat diet. Indian J Exp Biol 30, 292–294, 1992.1459596
Search in Google Scholar Back to article
Hinault C, Mothe-Satney I, Gautier N, Van Obberghen E. Amino acids require glucose to enhance, through phosphoinositide-dependent protein kinase 1, the insulin-activated protein kinase B cascade on insulin-resistant adipocytes. Diabetologia 49, 1017–1026, 2006.10.1007/s00125-006-0201-z16550357
Search in Google Scholar Back to article
Holten MK, Zacho M, Gaster M, Juel C, Wojtaszewski JF, Dela F. Strength training increases insulin-mediated glucose uptake, GLUT4 content, and insulin signaling in skeletal muscle in patients with type 2 diabetes. Diabetes 53, 294–305, 2004.10.2337/diabetes.53.2.29414747278
Search in Google Scholar Back to article
Hornberger TAJr, Farrar RP. Physiological hypertrophy of the FHL muscle following 8 weeks of progressive resistance exercise in the rat. Can J Appl Physiol 29, 16–31, 2004.10.1139/h04-00215001801
Open DOI Search in Google Scholar Back to article
Kraus WE, Houmard JA, Duscha BD, Knetzger KJ, Wharton MB, McCartney JS, Bales CW, Henes S, Samsa GP, Otvos JD, Kulkarni KR, Slentz CA. Effects of the amount and intensity of exercise on plasma lipoproteins. N Engl J Med 347, 1483–1492, 2002.10.1056/NEJMoa02019412421890
Search in Google Scholar Back to article
Lira FS, Yamashita AS, Uchida MC, Zanchi NE, Gualano B, Martins EJr, Caperuto EC, Seelaender M. Low and moderate, rather than high intensity strength exercise induces benefit regarding plasma lipid profile. Diabetol Metab Syndr 2, 31, 2010.10.1186/1758-5996-2-3120492685288877220492685
Open DOI Search in Google Scholar Back to article
Liu H, Liu R, Xiong Y, Li X, Wang X, Ma Y, Guo H, Hao L, Yao P, Liu L, Wang D, Yang X. Leucine facilitates the insulin-stimulated glucose uptake and insulin signaling in skeletal muscle cells: involving mTORC1 and mTORC2. Amino Acids 46, 1971–1979, 2014.10.1007/s00726-014-1752-924806638
Search in Google Scholar Back to article
Martins CEC, Lima VBS, Schoenfeld BJ, Tirapegui J. Effects of leucine supplementation and resistance training on myopathy of diabetic rats. Physiol Rep 5, e13273, 2017.10.14814/phy2.13273544955928536139
Search in Google Scholar Back to article
Mathe D. Dyslipidemia and diabetes: animal models. Diabete Metab 21,106–111, 1995.
Search in Google Scholar Back to article
Molanouri SM, Hassan ZM, Quinn LS, Gharakhanlou R, Baghersad L, Mahdavi M. Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype. Endocrine 49, 396–403, 2014.10.1007/s12020-014-0501-x25522723
Search in Google Scholar Back to article
Morifuji M, Sakai K, Sanbongi C, Sugiura K. Dietary whey protein downregulates fatty acid synthesis in the liver, but upregulates it in skeletal muscle of exercise-trained rats. Nutrition 21, 1052–1058, 2005.1615724310.1016/j.nut.2005.01.01016157243
Open DOI Search in Google Scholar Back to article
Nicastro H, da Luz CR, Chaves DF, das Neves W, Valente KS, Lancha AHJr. Leucine supplementation combined with resistance exercise improves the plasma lipid profile of dexamethasone-treated rats. Lipids Health Dis 11, 11:7, 2012.10.1186/1476-511X-11-7326808322236609
Open DOI Search in Google Scholar Back to article
Podrez EA. Anti-oxidant properties of high-density lipoprotein and atherosclerosis. Clin Exp Pharmacol Physiol 37, 719–725, 2010.10.1111/j.1440-1681.2010.05380.x301018420374263
Search in Google Scholar Back to article
Reeves PG, Nielsen FH, Fahey GCJr. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123, 1939–1951, 1993.10.1093/jn/123.11.19398229312
Search in Google Scholar Back to article
Safarzade A, Talebi-Garakani E. Short term resistance training enhanced plasma apoA-I and FABP4 levels in Streptozotocin-induced diabetic rats. J Diabetes Metab Disord 13, 41, 2014.10.1186/2251-6581-13-41394613024593955
Search in Google Scholar Back to article
Saravanan R, Pari L. Antihyperlipidemic and antiperoxidative effect of Diasulin, a polyherbal formulation in alloxan induced hyperglycemic rats. BMC Complement Altern Med 5, 14, 2005.10.1186/1472-6882-5-14118318815969768
Search in Google Scholar Back to article
Takada J, Machado MA, Peres SB, Brito LC, Borges-Silva CN, Costa CE, Fonseca-Alaniz MH, Andreotti S, Lima FB. Neonatal streptozotocin-induced diabetes mellitus: a model of insulin resistance associated with loss of adipose mass. Metabolism 56, 977–984, 2007.1757026110.1016/j.metabol.2006.05.02117570261
Open DOI Search in Google Scholar Back to article
Torres-Leal FL, Fonseca-Alaniz MH, Teodoro GF, de Capitani MD, Vianna D, Pantaleao LC, Matos-Neto EM, Rogero MM, Donato JJr, Tirapegui J. Leucine supplementation improves adiponectin and total cholesterol concentrations despite the lack of changes in adiposity or glucose homeostasis in rats previously exposed to a high-fat diet. Nutr Metab (Lond) 8, 62, 2011.10.1186/1743-7075-8-62318404321899736
Search in Google Scholar Back to article
Williams MA, Haskell WL, Ades PA, Amsterdam EA, Bittner V, Franklin BA, Gulanick M, Laing ST, Stewart KJ. Resistance exercise in individuals with and without cardiovascular disease: 2007 update: a scientific statement from the American Heart Association Council on Clinical Cardiology and Council on Nutrition, Physical Activity, and Metabolism. Circulation 116, 572–584, 2007.10.1161/CIRCULATIONAHA.107.18521417638929
Search in Google Scholar Back to article
Williams AD, Almond J, Ahuja KD, Beard DC, Robertson IK, Ball MJ. Cardiovascular and metabolic effects of community based resistance training in an older population. J Sci Med Sport 14, 331–337, 2011.10.1016/j.jsams.2011.02.01121441068
Open DOI Search in Google Scholar Back to article
Zhang Y, Guo K, LeBlanc RE, Loh D, Schwartz GJ, Yu YH. Increasing dietary leucine intake reduces diet-induced obesity and improves glucose and cholesterol metabolism in mice via multimechanisms. Diabetes 56, 1647–1654, 2007.1736097810.2337/db07-012317360978
Open DOI Search in Google Scholar Back to article

Resistance training alone or combined with leucine supplementation improves the serum lipid profile of diabetic rats, whereas leucine alone does not

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