Have a personal or library account? Click to login
Large scale propagation and in vitro weaning for the restoration of Viola palustris to support assisted colonisation of a threatened butterfly Cover

Large scale propagation and in vitro weaning for the restoration of Viola palustris to support assisted colonisation of a threatened butterfly

The EuroBiotech Journal
Volume 5 (2021): Issue 4 (October 2021)
By: Jonathan P. Kendon,  Alzbeta Novotna,  Margaret M. Ramsay,  Anne Porter and  Viswambharan Sarasan  
Open Access
|Oct 2021

Figures & Tables

Figure 1

Flow chart explaining stages from seedling production (highlighted in green)to transplantation to the field (highlighted in red). Substrates/conditions in which the plants are kept at the various stages are marked (agar, liquid medium, plug system subtrate and field conditions) along with the duration of each stage in days, weeks (wks) or months. Average multiplication rates are shown for agar, liquid and repeated bioreactor cycle stages.
Flow chart explaining stages from seedling production (highlighted in green)to transplantation to the field (highlighted in red). Substrates/conditions in which the plants are kept at the various stages are marked (agar, liquid medium, plug system subtrate and field conditions) along with the duration of each stage in days, weeks (wks) or months. Average multiplication rates are shown for agar, liquid and repeated bioreactor cycle stages.

Figure 2

Multiplication rate for Viola palustris cultured in semi-solid agar and liquid immersion system. Agar multiplication alone for 16 weeks; multiplication using RITA® for 10 days followed by 16 weeks on agar; 3 – multiplication using a second RITA® cycle for 10 days followed by 16 weeks on agar. Bars represent means of at least 28 explants +/- standard error. Bars with the same letter are not significantly different (P>0.05).
Multiplication rate for Viola palustris cultured in semi-solid agar and liquid immersion system. Agar multiplication alone for 16 weeks; multiplication using RITA® for 10 days followed by 16 weeks on agar; 3 – multiplication using a second RITA® cycle for 10 days followed by 16 weeks on agar. Bars represent means of at least 28 explants +/- standard error. Bars with the same letter are not significantly different (P>0.05).

Figure 3

Improved rooting of in vitro Viola palustris propagules using photoautotrophic plug substrate and sucrose-free liquid nutrient medium. a – agar culture; b – roots of agar-grown propagule; c – photoautotrophic plug propagule; d – photoautotrophic propagule with substrate removed to show robust root growth. Scale bars 1 cm.
Improved rooting of in vitro Viola palustris propagules using photoautotrophic plug substrate and sucrose-free liquid nutrient medium. a – agar culture; b – roots of agar-grown propagule; c – photoautotrophic plug propagule; d – photoautotrophic propagule with substrate removed to show robust root growth. Scale bars 1 cm.

Figure 4

Establishment rating measure for Viola palustris five months after potting up from photoautotrophic plug trays. A = 2, b = 3, c = 4, d = 5. No photograph is available for rating 1 because no pots achieved an establishment rating of 1, i.e. very poor or no establishment.
Establishment rating measure for Viola palustris five months after potting up from photoautotrophic plug trays. A = 2, b = 3, c = 4, d = 5. No photograph is available for rating 1 because no pots achieved an establishment rating of 1, i.e. very poor or no establishment.

Figure 5

Growth and establishment of ex vitro Viola palustris propagules in field sites after 16 months (2014 plantings) and 4 months (2015 plantings). Bars represent the average from a minimum of five colonies +/- standard error. b = significantly (P<0.05) lower ground cover than all other sites.
Growth and establishment of ex vitro Viola palustris propagules in field sites after 16 months (2014 plantings) and 4 months (2015 plantings). Bars represent the average from a minimum of five colonies +/- standard error. b = significantly (P<0.05) lower ground cover than all other sites.

Figure 6

Establishment of modules planted out in the field. a – 16 months after planting; b – 4 months after planting. Scale bar 5 cm.
Establishment of modules planted out in the field. a – 16 months after planting; b – 4 months after planting. Scale bar 5 cm.
DOI: https://doi.org/10.2478/ebtj-2021-0026 | Journal eISSN: 2564-615X
Journal RSS Feed
Language: English
Page range: 170 - 179
Published on: Oct 21, 2021
Published by: European Biotechnology Thematic Network Association
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Recovery,
In vitro weaning,
Food plant,
Photoautotrophic,
Community planting
Related subjects:
Life sciences,
Genetics,
Biotechnology,
Bioinformatics,
Life sciences, other

© 2021 Jonathan P. Kendon, Alzbeta Novotna, Margaret M. Ramsay, Anne Porter, Viswambharan Sarasan, published by European Biotechnology Thematic Network Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Previous articleVolume 5 (2021): Issue 4 (October 2021)Next article