Figure 1
Figure 2
Figure 3
For each given hour different CSF percentages were compared to each other_ Mean difference of each comparison and its statistical significance is indicated_ The degree of significance is indicated with the number of *, higher the number of * stronger the significance_
| Holm-Sidak’s multiple comparison test | Mean Diff. | Significant? | Summary |
|---|---|---|---|
| 24 Hours | |||
| 0 vs. 25 | -1496 | Yes | *** |
| 0 vs. 50 | -1238 | Yes | *** |
| 0 vs. 75 | -516.8 | No | ns |
| 0 vs. 100 | 392 | No | ns |
| 25 vs. 50 | 258.1 | No | ns |
| 25 vs. 75 | 979.5 | Yes | ** |
| 25 vs. 100 | 1888 | Yes | **** |
| 50 vs. 75 | 721.4 | Yes | * |
| 50 vs. 100 | 1630 | Yes | **** |
| 75 vs. 100 | 908.8 | Yes | ** |
| 48 Hours | |||
| 0 vs. 25 | -1260 | Yes | *** |
| 0 vs. 50 | -1338 | Yes | *** |
| 0 vs. 75 | -496.3 | No | ns |
| 0 vs. 100 | 908.9 | Yes | ** |
| 25 vs. 50 | -78.19 | No | ns |
| 25 vs. 75 | 763.9 | Yes | * |
| 25 vs. 100 | 2169 | Yes | **** |
| 50 vs. 75 | 842.1 | Yes | * |
| 50 vs. 100 | 2247 | Yes | **** |
| 75 vs. 100 | 1405 | Yes | *** |
| 72 Hours | |||
| 0 vs. 25 | -1159 | Yes | ** |
| 0 vs. 50 | -1301 | Yes | *** |
| 0 vs. 75 | -369.5 | No | ns |
| 0 vs. 100 | 1307 | Yes | *** |
| 25 vs. 50 | -142.1 | No | ns |
| 25 vs. 75 | 789 | Yes | * |
| 25 vs. 100 | 2466 | Yes | **** |
| 50 vs. 75 | 931.1 | Yes | ** |
| 50 vs. 100 | 2608 | Yes | **** |
| 75 vs. 100 | 1677 | Yes | **** |
Relative Luminescence Unit (RLU) values are shown for different CSF concentrations and different hours_ Same letters in the columns indicate no statistically significant difference (p>0_05)_ Same symbols in the rows indicate no statistically significant difference (p>0_05)_ The variability due to the CSF percentages was statistically significant_ Two Way RM ANOVA with Holm-Sidak multiple comparison test was performed for this analysis_
| TIME | ||||
|---|---|---|---|---|
| 24 Hours | 48 Hours | 72 Hours | ||
| Mean ± SEM | Mean ± SEM | Mean ± SEM | ||
| CSF Concentration (%) | 0 | 7237.93 ± 1536.22 a,d,# | 8324.95 ± 1982.12 a,& | 9103.60 ± 1653.54 a,* |
| 25 | 8734.22 ± 1678.76 b,c,# | 9585.13 ± 1942.57 b,c,& | 10262.11 ± 1698.34 b,c,* | |
| 50 | 8476.16 ± 1509.59 c,# | 9663.32 ± 2168.09 c,& | 10404.24 ± 1916.68 c,* | |
| 75 | 7754.74 ± 1708.62 a,# | 8821.20 ± 2048.36 a,& | 9473.12 ± 1802.25 a,* | |
| 100 | 6845.98 ± 1568.58 d,# | 7416.05 ± 1501.35 d,#,& | 7796.31 ± 1660.38 d,& | |
Comparisons of different time points within different CSF percentages_ Mean difference of each comparison and its statistical significance is indicated_ The degree of significance is indicated with the number of *, higher the number of * stronger the significance_
| Holm-Sidak’s multiple comparisons test | Mean Diff. | Significant? | Summary |
|---|---|---|---|
| 0% CSF | |||
| 24 Hours vs. 48 Hours | -1087 | Yes | *** |
| 24 Hours vs. 72 Hours | -1866 | Yes | **** |
| 48 Hours vs. 72 Hours | -778.6 | Yes | ** |
| 25% CSF | |||
| 24 Hours vs. 48 Hours | -850.9 | Yes | ** |
| 24 Hours vs. 72 Hours | -1528 | Yes | **** |
| 48 Hours vs. 72 Hours | -677 | Yes | * |
| 50% CSF | |||
| 24 Hours vs. 48 Hours | -1187 | Yes | *** |
| 24 Hours vs. 72 Hours | -1928 | Yes | **** |
| 48 Hours vs. 72 Hours | -740.9 | Yes | ** |
| 75% CSF | |||
| 24 Hours vs. 48 Hours | -1066 | Yes | *** |
| 24 Hours vs. 72 Hours | -1718 | Yes | **** |
| 48 Hours vs. 72 Hours | -651.9 | Yes | * |
| 100% CSF | |||
| 24 Hours vs. 48 Hours | -570.1 | No | ns |
| 24 Hours vs. 72 Hours | -950.3 | Yes | ** |
| 48 Hours vs. 72 Hours | -380.3 | No | ns |