IgA and IgG antibodies in SARS-CoV-2 vaccinated health workers by a homemade ELISA diagnostic test

	Results	Anti-S		Anti-N
	Results	IgG	IgA	IgG	IgA
1^st dose	Negative	7	16	15	18
	Borderline	3	0	1	0
	Positive	8	2	2	0
	total	18	18	18	18
2^nd dose	Negative	0	19	22	24
	Borderline	0	1	0	0
	Positive	24	4	2	0
	total	24	24	24	24
Zero time	Negative	2	2	1	2
	Borderline	0	0	0	0
	Positive	0	0	1	0
	total	2	2	2	2

Histograms show the characterization of serum of health workers: (A) anti-RBD ectodomain Spike protein IgG; (B) anti-nucleocapside protein IgG; (C) anti-RBD ectodomain Spike protein IgA; (D) anti-nucleocapside protein IgA.

Table 2

IgG and IgA levels in the serum of each health worker who underwent serological test.

				Anti-S (RBD) antibody				Anti-N antibody
				IgG Cut off: 0.277 OD		IgA Cut off: 0.290 OD		IgG Cut off: 0.326 OD		IgA Cut off: 0.381 OD
				OD index/cutoff	Result	OD index/cutoff	Result	OD index/cutoff	Result	OD index/cutoff	Result
		Vaccine Dose	Age	0.90-1.10	Result	0.90-1.10	Result	0.90-1.10	Result	0.90-1.10	Result
LT	♂	0	60	0.08	-	0.00	-	0.29	-	0.03	-
LT		I		0.93	+/-	0.70	-	0.38	-	0.03	-
LT		II		2.63	+	1.09	+/-	0.46	-	0.11	-
ARB	♀	I	61	3.16	+	0.10	-	0.42	-	0.00	-
ARB	♀	II	61	2.45	+	2.49	+	1.23	+	0.09	-
VD	♀	I	38	0.87	-	0.42	-	0.44	-	0.74	-
VD	♀	II	38	3.13	+	1.17	+	0.37	-	0.62	-
IDP	♀	I	40	1.92	+	0.23	-	0.59	-	0.14	-
IDP	♀	II	40	2.53	+	0.88	-	0.57	-	0.09	-
PM	♀	I	52	1.71	+	0.09	-	0.21	-	0.07	-
PM	♀	II	52	3.11	+	0.31	-	0.22	-	0.06	-
AM	♂	0	59	0.30	-	0.05	-	1.36	+	0.04	-
AM		I		2.84	+	0.19	-	1.04	+/-	0.18	-
AM		II		2.71	+	0.09	-	0.79	-	0.02	-
AC	♀	II	58	2.77	+	0.48	-	0.10	-	0.02	-
ACP	♀	II	28	2.29	+	0.41	-	0.40	-	0.03	-
MR	♀	I	56	3.01	+	1.70	+	0.17	-	0.08	-
MR	♀	II	56	2.27	+	2.30	+	0.13	-	0.16	-
MT	♀	I	56	0.26	-	0.23	-	0.67	-	0.09	-
MT	♀	II	56	3.49	+	0.36	-	0.87	-	0.07	-
MLV	♀	I	38	3.48	+	1.94	+	0.33	-	0.13	-
MLV	♀	II	38	2.51	+	1.13	+	0.08	-	0.01	-
RR	♂	I	35	1.09	+/-	0.10	-	0.09	-	0.00	-
RR	♂	II	35	2.77	+	0.63	-	0.15	-	0.04	-
GDA	♂	I	69	0.32	-	0.00	-	0.26	-	0.03	-
GDA	♂	II	69	2.88	+	0.61	-	0.23	-	0.02	-
BR	♂	I	42	0.83	-	0.09	-	0.23	-	0.06	-
BR	♂	II	42	2.53	+	0.72	-	0.31	-	0.05	-
CP	♀	I	53	2.53	+	0.29	-	0.49	-	0.13	-
CP	♀	II	53	2.87	+	0.28	-	0.11	-	0.04	-
MG	♀	I	47	0.41	-	0.00	-	0.17	-	-0.01	-
MG	♀	II	47	3.22	+	0.37	-	0.14	-	0.04	-
PT	♂	I	59	0.95	+/-	0.06	-	1.73	+	0.04	-
PT	♂	II	59	2.80	+	0.28	-	1.53	+	0.03	-
LM	♀	I	57	0.79	-	0.02	-	0.27	-	0.04	-
LM	♀	II	57	2.99	+	0.53	-	0.30	-	0.03	-
DS	♀	I	43	2.26	+	0.20	-	1.37	+	0.15	-
DS	♀	II	43	2.68	+	0.02	-	0.78	-	0.02	-
MF	♀	I	51	0.16	-	-0.04	-	0.10	-	-0.04	-
MF	♀	II	51	2.70	+	0.05	-	0.13	-	0.01	-
BS	♀	II	31	2.87	+	0.12	-	0.36	-	0.05	-
SE	♀	II		3.03	+	0.02	-	0.38	-	0.02	-
RA	♀	II	59	3.96	+	0.27	-	0.32	-	0.02	-
GAM	♀	II	65	3.04	+	0.17	-	0.10	-	0.06	-

Box & Whiskers plots show the serological level of IgG and IgA anti-RBD ectodomain Spike protein (A) and anti-nucleocapsid protein (B) of SARS-CoV-2. Tukey's multiple comparisons test was used (** P value < 0.005).

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DOI: https://doi.org/10.2478/ebtj-2021-0016 | Journal eISSN: 2564-615X

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Language: English

Page range: 13 - 19

Published on: Jun 17, 2021

Published by: European Biotechnology Thematic Network Association

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

ELISA,

IgG,

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© 2021 Tagliaferro Luigi, Menegazzi Paola, Cossa Luca Giulio, Maffia Michele, published by European Biotechnology Thematic Network Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 5 (2021): Issue s1 (June 2021)

IgA and IgG antibodies in SARS-CoV-2 vaccinated health workers by a homemade ELISA diagnostic test

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