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IgA and IgG antibodies in SARS-CoV-2 vaccinated health workers by a homemade ELISA diagnostic test Cover

IgA and IgG antibodies in SARS-CoV-2 vaccinated health workers by a homemade ELISA diagnostic test

The EuroBiotech Journal
Volume 5 (2021): Issue s1 (June 2021)
By: Tagliaferro Luigi,  Menegazzi Paola,  Cossa Luca Giulio and  Maffia Michele  
Open Access
|Jun 2021

Figures & Tables

Figure 1

Histograms show the characterization of serum of health workers: (A) anti-RBD ectodomain Spike protein IgG; (B) anti-nucleocapside protein IgG; (C) anti-RBD ectodomain Spike protein IgA; (D) anti-nucleocapside protein IgA.
Histograms show the characterization of serum of health workers: (A) anti-RBD ectodomain Spike protein IgG; (B) anti-nucleocapside protein IgG; (C) anti-RBD ectodomain Spike protein IgA; (D) anti-nucleocapside protein IgA.

Figure 2

Box & Whiskers plots show the serological level of IgG and IgA anti-RBD ectodomain Spike protein (A) and anti-nucleocapsid protein (B) of SARS-CoV-2. Tukey's multiple comparisons test was used (** P value < 0.005).
Box & Whiskers plots show the serological level of IgG and IgA anti-RBD ectodomain Spike protein (A) and anti-nucleocapsid protein (B) of SARS-CoV-2. Tukey's multiple comparisons test was used (** P value < 0.005).

IgG and IgA levels in the serum of each health worker who underwent serological test_

Anti-S (RBD) antibodyAnti-N antibody
IgG Cut off: 0.277 ODIgA Cut off: 0.290 ODIgG Cut off: 0.326 ODIgA Cut off: 0.381 OD
OD index/cutoffResultOD index/cutoffResultOD index/cutoffResultOD index/cutoffResult
Vaccine DoseAge0.90-1.100.90-1.100.90-1.100.90-1.10
LT♂0600.08-0.00-0.29-0.03-
LTI0.93+/-0.70-0.38-0.03-
LTII2.63+1.09+/-0.46-0.11-
ARB♀I613.16+0.10-0.42-0.00-
ARBII2.45+2.49+1.23+0.09-
VD♀I380.87-0.42-0.44-0.74-
VDII3.13+1.17+0.37-0.62-
IDP♀I401.92+0.23-0.59-0.14-
IDPII2.53+0.88-0.57-0.09-
PM♀I521.71+0.09-0.21-0.07-
PMII3.11+0.31-0.22-0.06-
AM♂0590.30-0.05-1.36+0.04-
AMI2.84+0.19-1.04+/-0.18-
AMII2.71+0.09-0.79-0.02-
AC♀II582.77+0.48-0.10-0.02-
ACP♀II282.29+0.41-0.40-0.03-
MR♀I563.01+1.70+0.17-0.08-
MRII2.27+2.30+0.13-0.16-
MT♀I560.26-0.23-0.67-0.09-
MTII3.49+0.36-0.87-0.07-
MLV♀I383.48+1.94+0.33-0.13-
MLVII2.51+1.13+0.08-0.01-
RR♂I351.09+/-0.10-0.09-0.00-
RRII2.77+0.63-0.15-0.04-
GDA♂I690.32-0.00-0.26-0.03-
GDAII2.88+0.61-0.23-0.02-
BR♂I420.83-0.09-0.23-0.06-
BRII2.53+0.72-0.31-0.05-
CP♀I532.53+0.29-0.49-0.13-
CPII2.87+0.28-0.11-0.04-
MG♀I470.41-0.00-0.17--0.01-
MGII3.22+0.37-0.14-0.04-
PT♂I590.95+/-0.06-1.73+0.04-
PTII2.80+0.28-1.53+0.03-
LM♀I570.79-0.02-0.27-0.04-
LMII2.99+0.53-0.30-0.03-
DS♀I432.26+0.20-1.37+0.15-
DSII2.68+0.02-0.78-0.02-
MF♀I510.16--0.04-0.10--0.04-
MFII2.70+0.05-0.13-0.01-
BS♀II312.87+0.12-0.36-0.05-
SE♀II 3.03+0.02-0.38-0.02-
RA♀II593.96+0.27-0.32-0.02-
GAM♀II653.04+0.17-0.10-0.06-

Characterization of the health workers' serum_

ResultsAnti-SAnti-N
IgGIgAIgGIgA
1st doseNegative7161518
Borderline3010
Positive8220
total18181818
2nd doseNegative0192224
Borderline0100
Positive24420
total24242424
Zero timeNegative2212
Borderline0000
Positive0010
total2222
DOI: https://doi.org/10.2478/ebtj-2021-0016 | Journal eISSN: 2564-615X
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Language: English
Page range: 13 - 19
Published on: Jun 17, 2021
Published by: European Biotechnology Thematic Network Association
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
COVID-19,
ELISA,
SARS-CoV-2,
IgG,
IgA
Related subjects:
Life sciences,
Genetics,
Biotechnology,
Bioinformatics,
Life sciences, other

© 2021 Tagliaferro Luigi, Menegazzi Paola, Cossa Luca Giulio, Maffia Michele, published by European Biotechnology Thematic Network Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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