In vitro production of sesquiterpenes by electro-elicitation in Gyrinops walla Gaetner calli and cell suspension cultures Cover

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In vitro production of sesquiterpenes by electro-elicitation in Gyrinops walla Gaetner calli and cell suspension cultures

The EuroBiotech Journal

Volume 5 (2021): Issue 2 (April 2021)

By: D. Sachithri P. Munasinghe, Seneviratnege Somaratne, Shyama R. Weerakoon and Chandani Ranasinghe

Open Access

|Apr 2021

Figures & Tables

Experiment set up for the electrostimulation for G. walla callus and cell suspension(a) diagram of the electro-elicitation apparatus for calli, (b) attached screw to adjust the distance between the callus and the wire electrode, (c) bottom part with aluminium disk and (d) diagram of electro-elicitation apparatus for cell suspension.

Electro-elicitation apparatus for calli which are electrically coupled in series.

Electro-elicitation apparatus for cell suspension which are electrically coupled in series.

TLC profiles of electro-elicited G. walla(a) calli1) 10 mA, 2) 50 mA on 8th week and (b) cell suspension3) 0.1 mA on 6th hour.

Mean area percentages of sesquiterpenes produced in G. walla calli under electric currents of (a) 10 mA and (b) 50mA.

Mean area percentages of sesquiterpenes produced in G. walla cell suspension under electric current 0.1 mA

Trends of area percentages of biologically elicited (a) G.walla calli and (b) cell suspension.
Note: the graphs included the data upto maximum area percentage of sesquiterpenes — Trends of area percentages of biologically elicited (a) G.walla calli and (b) cell suspension. Note: the graphs included the data upto maximum area percentage of sesquiterpenes

Effect of electro-elicitation G_ walla cell suspension

Intensity	Incubation period	Area percentage of
Intensity	Incubation period	γ-selinene	β-caryophyllene	α-guaiene
Control	0	0.01 (0.005)d	0.01 (0.005)^b	0.01 (0.005)^d
0.1	0.5	0.01 (0.003)^c	0.01 (0.005)^b	0.02 (0.001)^c
0.1	2	0.01 (0.005)^c	0.01 (0.005)b	0.09 (0.01)^b
0.1	4	0.02 (0.005)^bc	0.04 (0.005)^b	0.11 (0.01)^b
0.1	6	0.04 (0.005)^ab	0.05 (0.001)^a	0.16 (0.01)^a
0.1	12	0.03 (0.01)^a	0.02 (0.01)^b	0.10 (0.003)^b
0.1	24	0.01 (0.005)^d	0.01 (0.005)^b	0.08 (0.01)^b
0.1	72	0.01(0.005)^d	0.01 (0.005)^b	0.01 (0.005)^d

Effect of electro-elicitation on G_ walla calli

Intensity	Incubation period (weeks)	Area percentage of
Control		γ-selinne	β-caryophyllene	α-cardinol	α-guaiene
10	2	0.01 (0.005)^e	0.01 (0.01)^e	0.04 (0.005)^d	0.01 (0.005)^g
10	4	0.06 (0.04)^ed	0.01 (0.005)^e	0.06 (0.02)^d	0.07 (0.02)^de
10	6	0.11 (0.01)^ed	0.07 (0.02)^e	0.85 (0.05)^b	0.05 (0.03)^ef
10	8	0.25 (0.04)^bcde	1.33 (0.07)^a	0.86 (0.05)^a	0.13 (0.04)^b
10	10	0.19 (0.01)^ed	1.11 (0.04)^ab	0.48 (0.03)^bc	0.18 (0.01)^a
10	12	0.10 (0.01)^ed	0.09 (0.01)^e	0.07 (0.02)^d	0.05 (0.02)^de
10	14	0.08 (0.005)^ed	0.02 (0.002)^e	0.01 (0.004)^d	0.01 (0.006)^g
50	2	0.01 (0.003)^e	0.01 (0.001)^e	0.01 (0.005)^d	0.01 (0.005)^g
50	4	0.03 (0.02)^ed	0.85 (0.05)^d	0.03 (0.01)^d	0.01 (0.005)^g
50	6	0.42 (0.03)^cb	1.15 (0.05)^c	0.52 (0.05)^b	0.09 (0.01)^cd
50	8	0.46 (0.05)^b	1.37 (0.02)^bc	0.84 (0.02)^a	0.09 (0.005)^bc
50	10	0.88 (0.02)^bcd	1.04 (0.04)^cd	0.48 (0.03)^b	0.07 (0.02)^de
50	12	0.27 (0.02)^a	0.08 (0.02)^e	0.16 (0.01)^cd	0.02 (0.01)^gf
50	14	0.01 (0.005)^e	0.01 (0.01)^e	0.01 (0.004)^d	0.01 (0.006)^g

DOI: https://doi.org/10.2478/ebtj-2021-0011 | Journal eISSN: 2564-615X

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Language: English

Page range: 70 - 78

Published on: Apr 22, 2021

Published by: European Biotechnology Thematic Network Association

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

Sesquiterpenes,

sustainability,

in vitro production,

electro-elicitation

Related subjects:

Bioinformatics,

Life sciences, other

© 2021 D. Sachithri P. Munasinghe, Seneviratnege Somaratne, Shyama R. Weerakoon, Chandani Ranasinghe, published by European Biotechnology Thematic Network Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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